Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species
Microsatellites are codominant markers that, due to their high polymorphism, are a common choice for detecting genetic variability in various organisms, including fungi, plants, and animals. However, the process of developing these markers is both costly and time-consuming. As a result, the cross-sp...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/16/2/73 |
| _version_ | 1827343714719105024 |
|---|---|
| author | Dana Șuteu Mihai Pușcaș Ioan Băcilă Zoltán Robert Balázs Philippe Choler |
| author_facet | Dana Șuteu Mihai Pușcaș Ioan Băcilă Zoltán Robert Balázs Philippe Choler |
| author_sort | Dana Șuteu |
| collection | DOAJ |
| description | Microsatellites are codominant markers that, due to their high polymorphism, are a common choice for detecting genetic variability in various organisms, including fungi, plants, and animals. However, the process of developing these markers is both costly and time-consuming. As a result, the cross-species amplification has become a more rapid and more affordable alternative in biological studies. The objective of this study was to assess the applicability of 13 SSR markers, originally designed for <i>Carex curvula</i>, in other 14 species belonging to different sections of the genus. All the markers were successfully transferred with a mean of 90.76%, and 100% transferability was reached in two species (<i>C. baldensis</i> and <i>C. rupestris</i>). The lowest transferability was registered in the G165 marker, which did not produce amplification in six species. Together, the microsatellites amplified a total of 183 alleles, ranging from 10 to 19 alleles per locus, with an average of 14.07. The mean number of different alleles ranged from 0.846 to a maximum of 2.077 per locus. No significant departures from the Hardy–Weinberg equilibrium were detected in polymorphic loci. The transferability of the 13 SSR markers proved highly successful in various <i>Carex</i> species, across different clades and sections of the genus. |
| first_indexed | 2024-03-07T22:35:15Z |
| format | Article |
| id | doaj.art-1f2eaaf12a8e4d2abcc8de29a63bd994 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-07T22:35:15Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-1f2eaaf12a8e4d2abcc8de29a63bd9942024-02-23T15:14:00ZengMDPI AGDiversity1424-28182024-01-011627310.3390/d16020073Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> SpeciesDana Șuteu0Mihai Pușcaș1Ioan Băcilă2Zoltán Robert Balázs3Philippe Choler4Department of Experimental Biology, Institute of Biological Research, Branch of National Institute of Research and Development for Biological Sciences, 48 Republicii Street, 400015 Cluj-Napoca, RomaniaA. Borza Botanic Garden, Babeș-Bolyai University, 42 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Experimental Biology, Institute of Biological Research, Branch of National Institute of Research and Development for Biological Sciences, 48 Republicii Street, 400015 Cluj-Napoca, RomaniaCenter for Systematic Biology, Biodiversity and Bioresources—3B, Faculty of Biology and Geology, Babeș-Bolyai University, 1 Kogălniceanu Street, 400084 Cluj-Napoca, RomaniaUniversity Grenoble Alpes, University Savoie Mont Blanc, CNRS, LECA, F-38000 Grenoble, FranceMicrosatellites are codominant markers that, due to their high polymorphism, are a common choice for detecting genetic variability in various organisms, including fungi, plants, and animals. However, the process of developing these markers is both costly and time-consuming. As a result, the cross-species amplification has become a more rapid and more affordable alternative in biological studies. The objective of this study was to assess the applicability of 13 SSR markers, originally designed for <i>Carex curvula</i>, in other 14 species belonging to different sections of the genus. All the markers were successfully transferred with a mean of 90.76%, and 100% transferability was reached in two species (<i>C. baldensis</i> and <i>C. rupestris</i>). The lowest transferability was registered in the G165 marker, which did not produce amplification in six species. Together, the microsatellites amplified a total of 183 alleles, ranging from 10 to 19 alleles per locus, with an average of 14.07. The mean number of different alleles ranged from 0.846 to a maximum of 2.077 per locus. No significant departures from the Hardy–Weinberg equilibrium were detected in polymorphic loci. The transferability of the 13 SSR markers proved highly successful in various <i>Carex</i> species, across different clades and sections of the genus.https://www.mdpi.com/1424-2818/16/2/73<i>Carex</i> cladesgenetic polymorphismmicrosatellitesphylogenyphylogeography |
| spellingShingle | Dana Șuteu Mihai Pușcaș Ioan Băcilă Zoltán Robert Balázs Philippe Choler Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species Diversity <i>Carex</i> clades genetic polymorphism microsatellites phylogeny phylogeography |
| title | Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species |
| title_full | Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species |
| title_fullStr | Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species |
| title_full_unstemmed | Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species |
| title_short | Cross-Species Transferability of Specific SSR Markers from <i>Carex curvula</i> (Cyperaceae) to Other <i>Carex</i> Species |
| title_sort | cross species transferability of specific ssr markers from i carex curvula i cyperaceae to other i carex i species |
| topic | <i>Carex</i> clades genetic polymorphism microsatellites phylogeny phylogeography |
| url | https://www.mdpi.com/1424-2818/16/2/73 |
| work_keys_str_mv | AT danasuteu crossspeciestransferabilityofspecificssrmarkersfromicarexcurvulaicyperaceaetoothericarexispecies AT mihaipuscas crossspeciestransferabilityofspecificssrmarkersfromicarexcurvulaicyperaceaetoothericarexispecies AT ioanbacila crossspeciestransferabilityofspecificssrmarkersfromicarexcurvulaicyperaceaetoothericarexispecies AT zoltanrobertbalazs crossspeciestransferabilityofspecificssrmarkersfromicarexcurvulaicyperaceaetoothericarexispecies AT philippecholer crossspeciestransferabilityofspecificssrmarkersfromicarexcurvulaicyperaceaetoothericarexispecies |