Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines
To better serve breeding of broccoli, the electrophysiological, morphological and transcriptomic profiling of the isogenic Ogura-CMS, DGMS and their maintainer fertile lines, were carried out by scanning electron microscopy, investigation of agronomic traits and RNA-sequencing analysis. The agronomi...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/561 |
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| author | Zhansheng Li Lixiao Song Yumei Liu Fengqing Han Wei Liu |
| author_facet | Zhansheng Li Lixiao Song Yumei Liu Fengqing Han Wei Liu |
| author_sort | Zhansheng Li |
| collection | DOAJ |
| description | To better serve breeding of broccoli, the electrophysiological, morphological and transcriptomic profiling of the isogenic Ogura-CMS, DGMS and their maintainer fertile lines, were carried out by scanning electron microscopy, investigation of agronomic traits and RNA-sequencing analysis. The agronomic traits of plant height, length of the largest leaf, plant spread angle, single head weight, head width and stem diameter showed stronger performance in Ogura-CMS broccoli than in DGMS line or maintainer fertile line. However, the Ogura-CMS broccoli was poorer in the seed yield and seed germination than in the DGMS line and maintainer fertile line. Additionally, the DGMS broccoli had longer maturation and flowering periods than the Ogura-CMS and maintainer fertile lines. There were obvious differences in the honey gland, happening in the male sterility and fertile lines of broccoli. Additionally, the mechanism regulating Ogura-CMS and DGMS in broccoli was investigated using florets transcriptome analyses of the Ogura-CMS, DGMS and maintainer fertile lines. As a result, a total of 2670 differentially expressed genes (DEGs) were detected, including 1054 up- and 1616 downregulated genes in the Ogura-CMS and DGMS lines compared to the maintainer fertile line. A number of functionally known genes involved in plant hormones (auxin, salicylic acid and brassinosteroid), five Mitochondrial Oxidative Phosphorylation (OXPHOS) genes of <i>atp8</i>, <i>LOC106319879</i>, <i>LOC106324734</i>, <i>LOC106314622</i> and <i>LOC106298585</i>, and three upregulated genes (<i>Lhcb1</i>, <i>Lhcb3</i> and <i>Lhcb5</i>) associated with the photosynthesis-antenna protein pathway, were obviously detected to be highly associated with reproductive development including flowering time, maturity and reproductive period in the Ogura-CMS and DGMS broccoli comparing to their maintainer fertile line. Our research would provide a comprehensive foundation for understanding the differences of electrophysiological, morphological and transcriptomic profiles in the Ogura-CMS, DGMS and maintainer broccoli, and as well as being beneficial to exploring the mechanism of male sterility in <i>Brassica</i> crops. |
| first_indexed | 2024-03-09T21:11:46Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:11:46Z |
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| spelling | doaj.art-1f31652561134aa699e045c88d5ec5ca2023-11-23T21:43:25ZengMDPI AGPlants2223-77472022-02-0111456110.3390/plants11040561Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli LinesZhansheng Li0Lixiao Song1Yumei Liu2Fengqing Han3Wei Liu4Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing 100081, ChinaInstitute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing 100081, ChinaChina Vegetable Biotechnology (Shouguang) Co., Ltd., Shouguang 262700, ChinaTo better serve breeding of broccoli, the electrophysiological, morphological and transcriptomic profiling of the isogenic Ogura-CMS, DGMS and their maintainer fertile lines, were carried out by scanning electron microscopy, investigation of agronomic traits and RNA-sequencing analysis. The agronomic traits of plant height, length of the largest leaf, plant spread angle, single head weight, head width and stem diameter showed stronger performance in Ogura-CMS broccoli than in DGMS line or maintainer fertile line. However, the Ogura-CMS broccoli was poorer in the seed yield and seed germination than in the DGMS line and maintainer fertile line. Additionally, the DGMS broccoli had longer maturation and flowering periods than the Ogura-CMS and maintainer fertile lines. There were obvious differences in the honey gland, happening in the male sterility and fertile lines of broccoli. Additionally, the mechanism regulating Ogura-CMS and DGMS in broccoli was investigated using florets transcriptome analyses of the Ogura-CMS, DGMS and maintainer fertile lines. As a result, a total of 2670 differentially expressed genes (DEGs) were detected, including 1054 up- and 1616 downregulated genes in the Ogura-CMS and DGMS lines compared to the maintainer fertile line. A number of functionally known genes involved in plant hormones (auxin, salicylic acid and brassinosteroid), five Mitochondrial Oxidative Phosphorylation (OXPHOS) genes of <i>atp8</i>, <i>LOC106319879</i>, <i>LOC106324734</i>, <i>LOC106314622</i> and <i>LOC106298585</i>, and three upregulated genes (<i>Lhcb1</i>, <i>Lhcb3</i> and <i>Lhcb5</i>) associated with the photosynthesis-antenna protein pathway, were obviously detected to be highly associated with reproductive development including flowering time, maturity and reproductive period in the Ogura-CMS and DGMS broccoli comparing to their maintainer fertile line. Our research would provide a comprehensive foundation for understanding the differences of electrophysiological, morphological and transcriptomic profiles in the Ogura-CMS, DGMS and maintainer broccoli, and as well as being beneficial to exploring the mechanism of male sterility in <i>Brassica</i> crops.https://www.mdpi.com/2223-7747/11/4/561broccolicytoplasmic male sterilitydominant genic male sterilityagronomyRNA-sequencing |
| spellingShingle | Zhansheng Li Lixiao Song Yumei Liu Fengqing Han Wei Liu Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines Plants broccoli cytoplasmic male sterility dominant genic male sterility agronomy RNA-sequencing |
| title | Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines |
| title_full | Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines |
| title_fullStr | Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines |
| title_full_unstemmed | Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines |
| title_short | Electrophysiological, Morphologic, and Transcriptomic Profiling of the Ogura-CMS, DGMS and Maintainer Broccoli Lines |
| title_sort | electrophysiological morphologic and transcriptomic profiling of the ogura cms dgms and maintainer broccoli lines |
| topic | broccoli cytoplasmic male sterility dominant genic male sterility agronomy RNA-sequencing |
| url | https://www.mdpi.com/2223-7747/11/4/561 |
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