M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood
In experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negativ...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
«FIRMA «SILICEA» LLC
2004-04-01
|
| Series: | Российский кардиологический журнал |
| Subjects: | |
| Online Access: | https://russjcardiol.elpub.ru/jour/article/view/1961 |
| _version_ | 1797857270660333568 |
|---|---|
| author | V. I. Zirkin A. N. Trukhin E. N. Sizova S. A. Dvorjanskiy |
| author_facet | V. I. Zirkin A. N. Trukhin E. N. Sizova S. A. Dvorjanskiy |
| author_sort | V. I. Zirkin |
| collection | DOAJ |
| description | In experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negative inotropic effect, weakening chrono-inotropic correlations. Serum from umbilical blood in titer 1 : 100 (SUB-1: 100) did not affect contraction amplitude and chrono-inotropic correlations. But, due to endogenous blocker of M-cholinoreceptors (EMCRB), it decreased myocardial M-cholinoreactivity, demonstrating M-cholinoblocking properties. In particular, the serum shifted the dose-effect curve rightand downward, increasing acetylcholine Kd by more than 3 times. Serum acetylcholine affinity to M-CHR diminished, and M-cholinergic influence on the heart became less effective. The serum decreased acetylcholine potential (10-7 and 10-6 g/ml) to weaken chrono-inotropic correlations. In some cases, SUB-1: 100, due to endogenous M-cholinorecepor stabilizer (EMCRS), increased negative inotropic effect of acetylcholine. The nature of EMCRB and EMCRS, together with their possible normal and pathologic role in regulating the heart function, is discussed. |
| first_indexed | 2024-04-09T20:54:00Z |
| format | Article |
| id | doaj.art-1f324c0cb4694a90a702909f40a19921 |
| institution | Directory Open Access Journal |
| issn | 1560-4071 2618-7620 |
| language | Russian |
| last_indexed | 2024-04-09T20:54:00Z |
| publishDate | 2004-04-01 |
| publisher | «FIRMA «SILICEA» LLC |
| record_format | Article |
| series | Российский кардиологический журнал |
| spelling | doaj.art-1f324c0cb4694a90a702909f40a199212023-03-29T21:23:11Zrus«FIRMA «SILICEA» LLCРоссийский кардиологический журнал1560-40712618-76202004-04-010264691719M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical bloodV. I. Zirkin0A. N. Trukhin1E. N. Sizova2S. A. Dvorjanskiy3Кировская государственная медицинская академия; Вятский государственный гуманитарный университет; Вятский социально-экономический институтКировская государственная медицинская академия; Вятский государственный гуманитарный университет; Вятский социально-экономический институтКировская государственная медицинская академия; Вятский государственный гуманитарный университет; Вятский социально-экономический институтКировская государственная медицинская академия; Вятский государственный гуманитарный университет; Вятский социально-экономический институтIn experiments with isolated hearts from 31 frogs (R. ridibunda), acetylcholine (10-7, 10-6, 10-5g/ml) in a dose-dependent manner reduced contraction amplitude during electrostimulation (30 s, 1 Gz, 5 ms, 5-10 W). The time to achieve maximal amplitude did not change, that meant the serum had negative inotropic effect, weakening chrono-inotropic correlations. Serum from umbilical blood in titer 1 : 100 (SUB-1: 100) did not affect contraction amplitude and chrono-inotropic correlations. But, due to endogenous blocker of M-cholinoreceptors (EMCRB), it decreased myocardial M-cholinoreactivity, demonstrating M-cholinoblocking properties. In particular, the serum shifted the dose-effect curve rightand downward, increasing acetylcholine Kd by more than 3 times. Serum acetylcholine affinity to M-CHR diminished, and M-cholinergic influence on the heart became less effective. The serum decreased acetylcholine potential (10-7 and 10-6 g/ml) to weaken chrono-inotropic correlations. In some cases, SUB-1: 100, due to endogenous M-cholinorecepor stabilizer (EMCRS), increased negative inotropic effect of acetylcholine. The nature of EMCRB and EMCRS, together with their possible normal and pathologic role in regulating the heart function, is discussed.https://russjcardiol.elpub.ru/jour/article/view/1961frog miocardiumacetylcholineserum from umbilical bloodm3cholinoreativity dynamics |
| spellingShingle | V. I. Zirkin A. N. Trukhin E. N. Sizova S. A. Dvorjanskiy M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood Российский кардиологический журнал frog miocardium acetylcholine serum from umbilical blood m3cholinoreativity dynamics |
| title | M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| title_full | M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| title_fullStr | M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| title_full_unstemmed | M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| title_short | M-cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| title_sort | m cholinoreactivity dynamics in frog myocardium exposed to serum from human umbilical blood |
| topic | frog miocardium acetylcholine serum from umbilical blood m3cholinoreativity dynamics |
| url | https://russjcardiol.elpub.ru/jour/article/view/1961 |
| work_keys_str_mv | AT vizirkin mcholinoreactivitydynamicsinfrogmyocardiumexposedtoserumfromhumanumbilicalblood AT antrukhin mcholinoreactivitydynamicsinfrogmyocardiumexposedtoserumfromhumanumbilicalblood AT ensizova mcholinoreactivitydynamicsinfrogmyocardiumexposedtoserumfromhumanumbilicalblood AT sadvorjanskiy mcholinoreactivitydynamicsinfrogmyocardiumexposedtoserumfromhumanumbilicalblood |