Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy
A bHLH transcription factor that is induced by low temperature was found in apple (Malus × domestica Borkh.). To understand the sequence characteristics of the gene, bioinformatics analysis was performed. Furthermore, gene expression patterns of the laminated apple seeds and lateral flower buds were...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2016-03-01
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| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014116300346 |
| _version_ | 1797203963541454848 |
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| author | Yiran Ren Qiang Zhao Xianyan Zhao Yujin Hao Chunxiang You |
| author_facet | Yiran Ren Qiang Zhao Xianyan Zhao Yujin Hao Chunxiang You |
| author_sort | Yiran Ren |
| collection | DOAJ |
| description | A bHLH transcription factor that is induced by low temperature was found in apple (Malus × domestica Borkh.). To understand the sequence characteristics of the gene, bioinformatics analysis was performed. Furthermore, gene expression patterns of the laminated apple seeds and lateral flower buds were analyzed during the period of dormancy release with semi-quantitative RT-PCR. Based on secondary structure predictions, the results showed that the MdCIbHLH1 protein structure mainly included α-helix and random coil, while β-sheet and extended strand content was less. Semi-quantitative RT-PCR analysis showed that the expression patterns of MdCIbHLH1 were similar in laminated apple seeds and lateral flower buds during the period of dormancy release. Before dormancy release, expression levels of MdCIbHLH1 were high and gradually decreased during the period of dormancy release. These results indicated that MdCIbHLH1 might play an important role during dormancy release in apple seeds and apple buds. |
| first_indexed | 2024-04-24T08:27:41Z |
| format | Article |
| id | doaj.art-1f33bc18eb9d433e82147fa987d2641c |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:27:41Z |
| publishDate | 2016-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-1f33bc18eb9d433e82147fa987d2641c2024-04-16T21:06:59ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412016-03-0122616610.1016/j.hpj.2016.06.007Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of DormancyYiran RenQiang ZhaoXianyan ZhaoYujin HaoChunxiang YouA bHLH transcription factor that is induced by low temperature was found in apple (Malus × domestica Borkh.). To understand the sequence characteristics of the gene, bioinformatics analysis was performed. Furthermore, gene expression patterns of the laminated apple seeds and lateral flower buds were analyzed during the period of dormancy release with semi-quantitative RT-PCR. Based on secondary structure predictions, the results showed that the MdCIbHLH1 protein structure mainly included α-helix and random coil, while β-sheet and extended strand content was less. Semi-quantitative RT-PCR analysis showed that the expression patterns of MdCIbHLH1 were similar in laminated apple seeds and lateral flower buds during the period of dormancy release. Before dormancy release, expression levels of MdCIbHLH1 were high and gradually decreased during the period of dormancy release. These results indicated that MdCIbHLH1 might play an important role during dormancy release in apple seeds and apple buds.http://www.sciencedirect.com/science/article/pii/S2468014116300346appleMdCIbHLH1dormancyexpression analysis |
| spellingShingle | Yiran Ren Qiang Zhao Xianyan Zhao Yujin Hao Chunxiang You Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy Horticultural Plant Journal apple MdCIbHLH1 dormancy expression analysis |
| title | Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy |
| title_full | Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy |
| title_fullStr | Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy |
| title_full_unstemmed | Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy |
| title_short | Expression Analysis of the MdCIbHLH1 Gene in Apple Flower Buds and Seeds in the Process of Dormancy |
| title_sort | expression analysis of the mdcibhlh1 gene in apple flower buds and seeds in the process of dormancy |
| topic | apple MdCIbHLH1 dormancy expression analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2468014116300346 |
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