Optimization of the expression of levansucrase L17 in recombinant E. coli

Optimization of the expression of levansucrase L17 in recombinant E. coli

Levansucrases synthesize levans and fructooligosaccharides, which are of interest in the food and pharmaceutical industry. Leuconostoc mesenteroides Lm 17 produces levansucrase of about 120 kDa. The encoding gene from this strain was cloned and expressed in Escherichia coli BL21(DE3). The cloned gen...

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Bibliographic Details
Main Authors: Ilia Iliev, Tonka Vasileva, Veselin Bivolarski, Ayshe Salim, Sandrine Morel, Philippe Rabier, Valérie Gabriel
Format: Article
Language:English
Published: Taylor & Francis Group 2018-03-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Expression optimization
E. coli
Leuconostoc mesenteroides
levansucrase
low temperature
Online Access:http://dx.doi.org/10.1080/13102818.2018.1431056

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