Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice
<p>Abstract</p> <p>The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE<sup>−/−</sup> mice.</p> <p>Methods</p> <p>Eight-week-ol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-12-01
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| Series: | Lipids in Health and Disease |
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| Online Access: | http://www.lipidworld.com/content/11/1/166 |
| _version_ | 1818664758842228736 |
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| author | Sang Hui Yuan Na Yao Shutong Li Furong Wang Jiafu Fang Yongqi Qin Shucun |
| author_facet | Sang Hui Yuan Na Yao Shutong Li Furong Wang Jiafu Fang Yongqi Qin Shucun |
| author_sort | Sang Hui |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE<sup>−/−</sup> mice.</p> <p>Methods</p> <p>Eight-week-old male ApoE<sup>−/−</sup> mice were fed high fat diet (HFD) and treated with simvastatin (10 mg/kg per day), pinocembrin (20 mg/kg per day), or the combination therapy (simvastatin 5 mg/kg per day and pinocembrin 20 mg/kg per day) for 14 weeks. The serum lipid levels, nitric oxide (NO), endothelin (ET), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined with spectrophotometric measurement and ELISA assay. Vascular endothelial growth factor (VEGF) in serum and aortic root was detected. En face analyses of atherosclerotic lesion in whole aorta and aortic root sections were performed with plaque staining using oil red O.</p> <p>Results</p> <p>The combination treatment with simvastatin and pinocembrin resulted in significantly decreased levels of serum total cholesterol, triglycerides and low-density lipoprotein cholesterol, augmented NO levels and SOD activity, inhibited ET and VEGF expression. Immunohistochemistry of aortic valve sections revealed that the combination therapy also suppressed the expression of VEGF induced by HFD. In addition, HFD-induced arterial wall lipid disposition displayed by oil red O staining was reduced significantly in aortic root and whole aorta en face in the combination administrated mice. The effect of the combination was superior to simvastatin alone.</p> <p>Conclusion</p> <p>The combination of simvastatin and pinocembrin synergistically inhibited atherosclerotic lesion development in ApoE<sup>−/−</sup> mice with hyperlipidemia, which is partially dependent on the protective of vascular endothelium.</p> |
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| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-17T05:37:50Z |
| publishDate | 2012-12-01 |
| publisher | BMC |
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| series | Lipids in Health and Disease |
| spelling | doaj.art-1f37b99db5254d44a4991182815885b82022-12-21T22:01:33ZengBMCLipids in Health and Disease1476-511X2012-12-0111116610.1186/1476-511X-11-166Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient miceSang HuiYuan NaYao ShutongLi FurongWang JiafuFang YongqiQin Shucun<p>Abstract</p> <p>The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE<sup>−/−</sup> mice.</p> <p>Methods</p> <p>Eight-week-old male ApoE<sup>−/−</sup> mice were fed high fat diet (HFD) and treated with simvastatin (10 mg/kg per day), pinocembrin (20 mg/kg per day), or the combination therapy (simvastatin 5 mg/kg per day and pinocembrin 20 mg/kg per day) for 14 weeks. The serum lipid levels, nitric oxide (NO), endothelin (ET), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined with spectrophotometric measurement and ELISA assay. Vascular endothelial growth factor (VEGF) in serum and aortic root was detected. En face analyses of atherosclerotic lesion in whole aorta and aortic root sections were performed with plaque staining using oil red O.</p> <p>Results</p> <p>The combination treatment with simvastatin and pinocembrin resulted in significantly decreased levels of serum total cholesterol, triglycerides and low-density lipoprotein cholesterol, augmented NO levels and SOD activity, inhibited ET and VEGF expression. Immunohistochemistry of aortic valve sections revealed that the combination therapy also suppressed the expression of VEGF induced by HFD. In addition, HFD-induced arterial wall lipid disposition displayed by oil red O staining was reduced significantly in aortic root and whole aorta en face in the combination administrated mice. The effect of the combination was superior to simvastatin alone.</p> <p>Conclusion</p> <p>The combination of simvastatin and pinocembrin synergistically inhibited atherosclerotic lesion development in ApoE<sup>−/−</sup> mice with hyperlipidemia, which is partially dependent on the protective of vascular endothelium.</p>http://www.lipidworld.com/content/11/1/166PinocembrinSimvastatinCombined therapyAtherosclerotic lesionApolipoprotein E knockout mice |
| spellingShingle | Sang Hui Yuan Na Yao Shutong Li Furong Wang Jiafu Fang Yongqi Qin Shucun Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice Lipids in Health and Disease Pinocembrin Simvastatin Combined therapy Atherosclerotic lesion Apolipoprotein E knockout mice |
| title | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
| title_full | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
| title_fullStr | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
| title_full_unstemmed | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
| title_short | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
| title_sort | inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoe deficient mice |
| topic | Pinocembrin Simvastatin Combined therapy Atherosclerotic lesion Apolipoprotein E knockout mice |
| url | http://www.lipidworld.com/content/11/1/166 |
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