Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro
Soil salinity is one of the most common abiotic stressors that affects plant growth and development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP), a donor of nitric oxide (NO), on the physiological response of common centaury (<i>Centaurium erythraea</...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2075-1729/13/1/154 |
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| author | Milana Trifunović-Momčilov Nikola Stamenković Marija Đurić Snežana Milošević Marija Marković Zlatko Giba Angelina Subotić |
| author_facet | Milana Trifunović-Momčilov Nikola Stamenković Marija Đurić Snežana Milošević Marija Marković Zlatko Giba Angelina Subotić |
| author_sort | Milana Trifunović-Momčilov |
| collection | DOAJ |
| description | Soil salinity is one of the most common abiotic stressors that affects plant growth and development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP), a donor of nitric oxide (NO), on the physiological response of common centaury (<i>Centaurium erythraea</i>) shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and 250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt stress. However, MDA concentration was decreased, while H<sub>2</sub>O<sub>2</sub> and proline content did not drastically change under the stress conditions caused by NaCl. Exogenous application of SNP altered the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic pigment content, total phenolics and proline content were noted, with reduced MDA, but not H<sub>2</sub>O<sub>2</sub>, concentration was observed. In addition, the exogenous application of SNP increased the degree of DPPH reduction as well as SOD, CAT and POX activities. |
| first_indexed | 2024-03-09T11:56:16Z |
| format | Article |
| id | doaj.art-1f3904f0fbd3412499745a2a1afec313 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T11:56:16Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-1f3904f0fbd3412499745a2a1afec3132023-11-30T23:08:45ZengMDPI AGLife2075-17292023-01-0113115410.3390/life13010154Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In VitroMilana Trifunović-Momčilov0Nikola Stamenković1Marija Đurić2Snežana Milošević3Marija Marković4Zlatko Giba5Angelina Subotić6Department for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaDepartment for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaDepartment for Plant Physiology, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, SerbiaSoil salinity is one of the most common abiotic stressors that affects plant growth and development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP), a donor of nitric oxide (NO), on the physiological response of common centaury (<i>Centaurium erythraea</i>) shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and 250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt stress. However, MDA concentration was decreased, while H<sub>2</sub>O<sub>2</sub> and proline content did not drastically change under the stress conditions caused by NaCl. Exogenous application of SNP altered the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic pigment content, total phenolics and proline content were noted, with reduced MDA, but not H<sub>2</sub>O<sub>2</sub>, concentration was observed. In addition, the exogenous application of SNP increased the degree of DPPH reduction as well as SOD, CAT and POX activities.https://www.mdpi.com/2075-1729/13/1/154common centaurysalinity stressoxidative stressantioxidative protectionsodium nitroprusside |
| spellingShingle | Milana Trifunović-Momčilov Nikola Stamenković Marija Đurić Snežana Milošević Marija Marković Zlatko Giba Angelina Subotić Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro Life common centaury salinity stress oxidative stress antioxidative protection sodium nitroprusside |
| title | Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro |
| title_full | Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro |
| title_fullStr | Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro |
| title_full_unstemmed | Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro |
| title_short | Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (<i>Centaurium erythraea</i> Rafn) Shoots Grown In Vitro |
| title_sort | role of sodium nitroprusside on potential mitigation of salt stress in centaury i centaurium erythraea i rafn shoots grown in vitro |
| topic | common centaury salinity stress oxidative stress antioxidative protection sodium nitroprusside |
| url | https://www.mdpi.com/2075-1729/13/1/154 |
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