Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation
Although glucocorticoids are highly effective in treating various types of inflammation such as skin disease, rheumatic disease, and allergic disease, their application have been seriously limited for their high incidence of side effects, particularly in long term treatment. To improve efficacy and...
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MDPI AG
2018-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/10/4/286 |
| _version_ | 1811297660540289024 |
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| author | Xiumei Zhang Mingfeng Qiu Pengcheng Guo Yumei Lian Enge Xu Jing Su |
| author_facet | Xiumei Zhang Mingfeng Qiu Pengcheng Guo Yumei Lian Enge Xu Jing Su |
| author_sort | Xiumei Zhang |
| collection | DOAJ |
| description | Although glucocorticoids are highly effective in treating various types of inflammation such as skin disease, rheumatic disease, and allergic disease, their application have been seriously limited for their high incidence of side effects, particularly in long term treatment. To improve efficacy and reduce side effects, we encapsulated betamethasone phosphate (BSP) into biocompatible red blood cells (RBCs) and explored its long acting-effect. BSP was loaded into rat autologous erythrocytes by hypotonic preswelling method, and the loading amount was about 2.5 mg/mL cells. In vitro, BSP loaded RBCs (BSP-RBCs) presented similar morphology, osmotic fragility to native RBCs (NRBCs). After the loading process, the loaded cells can maintain around 70% of Na<sup>+</sup>/K<sup>+</sup>-ATPase activity of natural cells. In vivo, a series of tests including survival, pharmacokinetics, and anti-inflammatory effect were carried out to examine the long-acting effect of BSP-RBCs. The results shown that the loaded cells could circulate in plasma for over nine days, the release of BSP can last for over seven days and the anti-inflammatory effect can still be observed on day 5 after injection. Totally, BSP-loaded autologous erythrocytes seem to be a promising sustained releasing delivery system with long anti-inflammatory effect. |
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| format | Article |
| id | doaj.art-1f3b806dff6d4141b7e081e33a494906 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-13T06:07:41Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-1f3b806dff6d4141b7e081e33a4949062022-12-22T02:59:11ZengMDPI AGPharmaceutics1999-49232018-12-0110428610.3390/pharmaceutics10040286pharmaceutics10040286Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-InflammationXiumei Zhang0Mingfeng Qiu1Pengcheng Guo2Yumei Lian3Enge Xu4Jing Su5School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaAlthough glucocorticoids are highly effective in treating various types of inflammation such as skin disease, rheumatic disease, and allergic disease, their application have been seriously limited for their high incidence of side effects, particularly in long term treatment. To improve efficacy and reduce side effects, we encapsulated betamethasone phosphate (BSP) into biocompatible red blood cells (RBCs) and explored its long acting-effect. BSP was loaded into rat autologous erythrocytes by hypotonic preswelling method, and the loading amount was about 2.5 mg/mL cells. In vitro, BSP loaded RBCs (BSP-RBCs) presented similar morphology, osmotic fragility to native RBCs (NRBCs). After the loading process, the loaded cells can maintain around 70% of Na<sup>+</sup>/K<sup>+</sup>-ATPase activity of natural cells. In vivo, a series of tests including survival, pharmacokinetics, and anti-inflammatory effect were carried out to examine the long-acting effect of BSP-RBCs. The results shown that the loaded cells could circulate in plasma for over nine days, the release of BSP can last for over seven days and the anti-inflammatory effect can still be observed on day 5 after injection. Totally, BSP-loaded autologous erythrocytes seem to be a promising sustained releasing delivery system with long anti-inflammatory effect.https://www.mdpi.com/1999-4923/10/4/286red blood cellsdrug delivery systembetamethasone phosphatelong anti-inflammation |
| spellingShingle | Xiumei Zhang Mingfeng Qiu Pengcheng Guo Yumei Lian Enge Xu Jing Su Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation Pharmaceutics red blood cells drug delivery system betamethasone phosphate long anti-inflammation |
| title | Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation |
| title_full | Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation |
| title_fullStr | Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation |
| title_full_unstemmed | Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation |
| title_short | Autologous Red Blood Cell Delivery of Betamethasone Phosphate Sodium for Long Anti-Inflammation |
| title_sort | autologous red blood cell delivery of betamethasone phosphate sodium for long anti inflammation |
| topic | red blood cells drug delivery system betamethasone phosphate long anti-inflammation |
| url | https://www.mdpi.com/1999-4923/10/4/286 |
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