Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance
Genome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of <i>Helicoverpa armigera</i> were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a subl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/15/3/146 |
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| author | Md-Mafizur Rahman Celso Omoto Juil Kim |
| author_facet | Md-Mafizur Rahman Celso Omoto Juil Kim |
| author_sort | Md-Mafizur Rahman |
| collection | DOAJ |
| description | Genome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of <i>Helicoverpa armigera</i> were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a sublethal dose of deltamethrin was administered to a Korean-resistant strain (Kor-T). The average length of lncRNAs was approximately 531 bp, and the expression ratio of lncRNAs was 28% of the total RNA. The identified lncRNAs were divided into six categories—intronic, intergenic, sense, antisense, cis-RNA, and trans-RNA—based on their location and mechanism of action. Intergenic and intronic lncRNA transcripts were the most abundant (38% and 33%, respectively). Further, 828 detoxification-related lncRNAs were selected using the Gene Ontology analysis. The cytochrome P450-related lncRNA expression levels were significantly higher in susceptible strains than in resistant strains. In contrast, cuticle protein-related lncRNA expression levels were significantly higher in all resistant strains than in susceptible strains. Our findings suggest that certain lncRNAs contribute to the downregulation of insecticide resistance-related P450 genes in susceptible strains, whereas other lncRNAs may be involved in the overexpression of cuticle protein genes, potentially affecting the pyrethroid resistance mechanism. |
| first_indexed | 2024-04-24T18:09:59Z |
| format | Article |
| id | doaj.art-1f3c5b19a9a848138eadbbabd9f93c97 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-04-24T18:09:59Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-1f3c5b19a9a848138eadbbabd9f93c972024-03-27T13:47:10ZengMDPI AGInsects2075-44502024-02-0115314610.3390/insects15030146Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide ResistanceMd-Mafizur Rahman0Celso Omoto1Juil Kim2Agriculture and Life Science Research Institute, Kangwon National University, Chuncheon 24341, Republic of KoreaDepartment of Entomology and Acarology, Luiz de Queiroz College of Agriculture (ESALQ), University of Sao Paulo, Piracicaba 13418-900, BrazilAgriculture and Life Science Research Institute, Kangwon National University, Chuncheon 24341, Republic of KoreaGenome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of <i>Helicoverpa armigera</i> were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a sublethal dose of deltamethrin was administered to a Korean-resistant strain (Kor-T). The average length of lncRNAs was approximately 531 bp, and the expression ratio of lncRNAs was 28% of the total RNA. The identified lncRNAs were divided into six categories—intronic, intergenic, sense, antisense, cis-RNA, and trans-RNA—based on their location and mechanism of action. Intergenic and intronic lncRNA transcripts were the most abundant (38% and 33%, respectively). Further, 828 detoxification-related lncRNAs were selected using the Gene Ontology analysis. The cytochrome P450-related lncRNA expression levels were significantly higher in susceptible strains than in resistant strains. In contrast, cuticle protein-related lncRNA expression levels were significantly higher in all resistant strains than in susceptible strains. Our findings suggest that certain lncRNAs contribute to the downregulation of insecticide resistance-related P450 genes in susceptible strains, whereas other lncRNAs may be involved in the overexpression of cuticle protein genes, potentially affecting the pyrethroid resistance mechanism.https://www.mdpi.com/2075-4450/15/3/146<i>Helicoverpa armigera</i>insecticide resistancelncRNAscytochrome P450RNAseq |
| spellingShingle | Md-Mafizur Rahman Celso Omoto Juil Kim Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance Insects <i>Helicoverpa armigera</i> insecticide resistance lncRNAs cytochrome P450 RNAseq |
| title | Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance |
| title_full | Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance |
| title_fullStr | Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance |
| title_full_unstemmed | Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance |
| title_short | Genome-Wide Exploration of Long Non-Coding RNAs of <i>Helicoverpa armigera</i> in Response to Pyrethroid Insecticide Resistance |
| title_sort | genome wide exploration of long non coding rnas of i helicoverpa armigera i in response to pyrethroid insecticide resistance |
| topic | <i>Helicoverpa armigera</i> insecticide resistance lncRNAs cytochrome P450 RNAseq |
| url | https://www.mdpi.com/2075-4450/15/3/146 |
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