MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i>
MicroRNAs (miRNAs) are non-coding RNAs, which, as members of the RNA interference pathway, play a pivotal role in antiviral infection. Almost 80% of plant viruses are transmitted by insect vectors; however, little is known about the interaction of the miRNAs of insect vectors with plant viruses. Her...
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MDPI AG
2024-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/1/97 |
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| author | Haitao Wang Yan Dong Qiufang Xu Man Wang Shuo Li Yinghua Ji |
| author_facet | Haitao Wang Yan Dong Qiufang Xu Man Wang Shuo Li Yinghua Ji |
| author_sort | Haitao Wang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are non-coding RNAs, which, as members of the RNA interference pathway, play a pivotal role in antiviral infection. Almost 80% of plant viruses are transmitted by insect vectors; however, little is known about the interaction of the miRNAs of insect vectors with plant viruses. Here, we took rice black-streaked dwarf virus (RBSDV), a devastating virus to rice production in eastern Asia, and the small brown planthopper, (SBPH, <i>Laodelphax striatellus</i>) as a model to investigate the role of microRNA750-3p (miR750-3p) in regulating viral transmission. Our results showed that Ls-miR750-3p was downregulated in RBSDV-infected SBPH and predominately expressed in the midgut of SBPH. Injection with miR750-3p agomir significantly reduced viral accumulation, and the injection with the miR750-3p inhibitor, antagomir-750-3p, dramatically promoted the viral accumulation in SBPH, as detected using RT-qPCR and Western blotting. The <i>processing of precursor 7</i> (<i>POP7</i>), a subunit of RNase P and RNase MRP, was screened, identified, and verified using a dual luciferase reporter assay as one target of miR750-3p. Knockdown of <i>POP7</i> notably increased RBSDV viral propagation in SBPH and then increased the viral transmission rate by SBPH. Taken together, our data indicate that miR750-3p targets <i>POP7</i> to suppress RBSDV infection in its insect vector. These results enriched the role of POP7 in modulating virus infection in host insects and shared new insight into the function of miRNAs in plant virus and insect vector interaction. |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T10:32:12Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1f3d1b12b19f4adc8834b8a84bdd29332024-01-26T18:48:11ZengMDPI AGViruses1999-49152024-01-011619710.3390/v16010097MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i>Haitao Wang0Yan Dong1Qiufang Xu2Man Wang3Shuo Li4Yinghua Ji5Institute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Plant Protection, Key Laboratory of Food Quality and Safety of Jiangsu Province, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaMicroRNAs (miRNAs) are non-coding RNAs, which, as members of the RNA interference pathway, play a pivotal role in antiviral infection. Almost 80% of plant viruses are transmitted by insect vectors; however, little is known about the interaction of the miRNAs of insect vectors with plant viruses. Here, we took rice black-streaked dwarf virus (RBSDV), a devastating virus to rice production in eastern Asia, and the small brown planthopper, (SBPH, <i>Laodelphax striatellus</i>) as a model to investigate the role of microRNA750-3p (miR750-3p) in regulating viral transmission. Our results showed that Ls-miR750-3p was downregulated in RBSDV-infected SBPH and predominately expressed in the midgut of SBPH. Injection with miR750-3p agomir significantly reduced viral accumulation, and the injection with the miR750-3p inhibitor, antagomir-750-3p, dramatically promoted the viral accumulation in SBPH, as detected using RT-qPCR and Western blotting. The <i>processing of precursor 7</i> (<i>POP7</i>), a subunit of RNase P and RNase MRP, was screened, identified, and verified using a dual luciferase reporter assay as one target of miR750-3p. Knockdown of <i>POP7</i> notably increased RBSDV viral propagation in SBPH and then increased the viral transmission rate by SBPH. Taken together, our data indicate that miR750-3p targets <i>POP7</i> to suppress RBSDV infection in its insect vector. These results enriched the role of POP7 in modulating virus infection in host insects and shared new insight into the function of miRNAs in plant virus and insect vector interaction.https://www.mdpi.com/1999-4915/16/1/97microRNA750-3pprocessing of precursor 7rice black-streaked dwarf virussmall brown planthopper<i>Laodelphax striatellus</i>viral propagation |
| spellingShingle | Haitao Wang Yan Dong Qiufang Xu Man Wang Shuo Li Yinghua Ji MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> Viruses microRNA750-3p processing of precursor 7 rice black-streaked dwarf virus small brown planthopper <i>Laodelphax striatellus</i> viral propagation |
| title | MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> |
| title_full | MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> |
| title_fullStr | MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> |
| title_full_unstemmed | MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> |
| title_short | MicroRNA750-3p Targets <i>Processing of Precursor 7</i> to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector <i>Laodelphax striatellus</i> |
| title_sort | microrna750 3p targets i processing of precursor 7 i to suppress rice black streaked dwarf virus propagation in vector i laodelphax striatellus i |
| topic | microRNA750-3p processing of precursor 7 rice black-streaked dwarf virus small brown planthopper <i>Laodelphax striatellus</i> viral propagation |
| url | https://www.mdpi.com/1999-4915/16/1/97 |
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