Regulated growth of diatom cells on self-assembled monolayers

<p>Abstract</p> <p>We succeeded in regulating the growth of diatom cells on chemically modified glass surfaces. Glass surfaces were functionalized with -CF<sub>3</sub>, -CH<sub>3</sub>, -COOH, and -NH<sub>2 </sub>groups using the technique of sel...

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Main Authors: Kobayashi Koichi, Maeda Yuta, Yamada Tomoaki, Umemura Kazuo, Kuroda Reiko, Mayama Shigeki
Format: Article
Language:English
Published: BMC 2007-03-01
Series:Journal of Nanobiotechnology
Online Access:http://www.jnanobiotechnology.com/content/5/1/2
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author Kobayashi Koichi
Maeda Yuta
Yamada Tomoaki
Umemura Kazuo
Kuroda Reiko
Mayama Shigeki
author_facet Kobayashi Koichi
Maeda Yuta
Yamada Tomoaki
Umemura Kazuo
Kuroda Reiko
Mayama Shigeki
author_sort Kobayashi Koichi
collection DOAJ
description <p>Abstract</p> <p>We succeeded in regulating the growth of diatom cells on chemically modified glass surfaces. Glass surfaces were functionalized with -CF<sub>3</sub>, -CH<sub>3</sub>, -COOH, and -NH<sub>2 </sub>groups using the technique of self-assembled monolayers (SAM), and diatom cells were subsequently cultured on these surfaces. When the samples were rinsed after the adhesion of the diatom cells on the modified surfaces, the diatoms formed two dimensional arrays; this was not possible without the rinsing treatment. Furthermore, we examined the number of cells that grew and their motility by time-lapse imaging in order to clarify the interaction between the cells and SAMs. We hope that our results will be a basis for developing biodevices using living photosynthetic diatom cells.</p>
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spelling doaj.art-1f44917f8e8e44908ff147908d2c55ea2022-12-22T02:54:11ZengBMCJournal of Nanobiotechnology1477-31552007-03-0151210.1186/1477-3155-5-2Regulated growth of diatom cells on self-assembled monolayersKobayashi KoichiMaeda YutaYamada TomoakiUmemura KazuoKuroda ReikoMayama Shigeki<p>Abstract</p> <p>We succeeded in regulating the growth of diatom cells on chemically modified glass surfaces. Glass surfaces were functionalized with -CF<sub>3</sub>, -CH<sub>3</sub>, -COOH, and -NH<sub>2 </sub>groups using the technique of self-assembled monolayers (SAM), and diatom cells were subsequently cultured on these surfaces. When the samples were rinsed after the adhesion of the diatom cells on the modified surfaces, the diatoms formed two dimensional arrays; this was not possible without the rinsing treatment. Furthermore, we examined the number of cells that grew and their motility by time-lapse imaging in order to clarify the interaction between the cells and SAMs. We hope that our results will be a basis for developing biodevices using living photosynthetic diatom cells.</p>http://www.jnanobiotechnology.com/content/5/1/2
spellingShingle Kobayashi Koichi
Maeda Yuta
Yamada Tomoaki
Umemura Kazuo
Kuroda Reiko
Mayama Shigeki
Regulated growth of diatom cells on self-assembled monolayers
Journal of Nanobiotechnology
title Regulated growth of diatom cells on self-assembled monolayers
title_full Regulated growth of diatom cells on self-assembled monolayers
title_fullStr Regulated growth of diatom cells on self-assembled monolayers
title_full_unstemmed Regulated growth of diatom cells on self-assembled monolayers
title_short Regulated growth of diatom cells on self-assembled monolayers
title_sort regulated growth of diatom cells on self assembled monolayers
url http://www.jnanobiotechnology.com/content/5/1/2
work_keys_str_mv AT kobayashikoichi regulatedgrowthofdiatomcellsonselfassembledmonolayers
AT maedayuta regulatedgrowthofdiatomcellsonselfassembledmonolayers
AT yamadatomoaki regulatedgrowthofdiatomcellsonselfassembledmonolayers
AT umemurakazuo regulatedgrowthofdiatomcellsonselfassembledmonolayers
AT kurodareiko regulatedgrowthofdiatomcellsonselfassembledmonolayers
AT mayamashigeki regulatedgrowthofdiatomcellsonselfassembledmonolayers