Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique
Background and Objective: Enterotoxigenic Escherichia coli (ETEC) are the most common agent which causes diarrhea, worldwide. ETEC is colonized along the cells and then producing heat-labile (LT) and heat-stable enterotoxigenic which enter into intestinal epithelial cells and causes water and electr...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2015-09-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-852&slc_lang=en&sid=1 |
| _version_ | 1818488953605455872 |
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| author | Esfandiari P Amani J Imani Fooladi AA Forghanifard MM Mirhossaini SA |
| author_facet | Esfandiari P Amani J Imani Fooladi AA Forghanifard MM Mirhossaini SA |
| author_sort | Esfandiari P |
| collection | DOAJ |
| description | Background and Objective: Enterotoxigenic Escherichia coli (ETEC) are the most common agent which causes diarrhea, worldwide. ETEC is colonized along the cells and then producing heat-labile (LT) and heat-stable enterotoxigenic which enter into intestinal epithelial cells and causes water and electrolyte loss from intestinal epithelial cells and eventually cause diarrhea.This study was done to detect the heat-labile toxin in Enterotoxigenic Escherichia coli using PCR-ELISA technique. Methods: In this descriptive study, DIG-labeled PCR products were bounded to streptoavidin-coated wells of a microtiter plate and detected by anti-DIG–peroxidase conjugate. The biotin-labeled internal probe was used for verification of PCR products. Results: Heat-labile toxin was detected by PCR-ELISA method. The sensitivity of heat-labile toxin was 1.9 ng. This method did not cross-react with bacteria from this variety. Conclusion: PCR-ELISA method is 100 times more sensitive than conventional PCR method and due to lack of agarose gel and electrophoresis device it can be a good alternative to traditional method. |
| first_indexed | 2024-12-10T16:57:40Z |
| format | Article |
| id | doaj.art-1f4762ea3aea4a068cc7cb64b6bb5560 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-10T16:57:40Z |
| publishDate | 2015-09-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-1f4762ea3aea4a068cc7cb64b6bb55602022-12-22T01:40:40ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802015-09-01173114121Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA techniqueEsfandiari P0Amani J1Imani Fooladi AA2Forghanifard MM3Mirhossaini SA4 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran and Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Associate Professor, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Associate Professor, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Assistant Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Ph.D Candidate in Bacterial Toxin, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Background and Objective: Enterotoxigenic Escherichia coli (ETEC) are the most common agent which causes diarrhea, worldwide. ETEC is colonized along the cells and then producing heat-labile (LT) and heat-stable enterotoxigenic which enter into intestinal epithelial cells and causes water and electrolyte loss from intestinal epithelial cells and eventually cause diarrhea.This study was done to detect the heat-labile toxin in Enterotoxigenic Escherichia coli using PCR-ELISA technique. Methods: In this descriptive study, DIG-labeled PCR products were bounded to streptoavidin-coated wells of a microtiter plate and detected by anti-DIG–peroxidase conjugate. The biotin-labeled internal probe was used for verification of PCR products. Results: Heat-labile toxin was detected by PCR-ELISA method. The sensitivity of heat-labile toxin was 1.9 ng. This method did not cross-react with bacteria from this variety. Conclusion: PCR-ELISA method is 100 times more sensitive than conventional PCR method and due to lack of agarose gel and electrophoresis device it can be a good alternative to traditional method.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-852&slc_lang=en&sid=1Enterotoxigenic Escherichia coliPCR-ELISAHeat-labile enterotoxin |
| spellingShingle | Esfandiari P Amani J Imani Fooladi AA Forghanifard MM Mirhossaini SA Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique مجله دانشگاه علوم پزشکی گرگان Enterotoxigenic Escherichia coli PCR-ELISA Heat-labile enterotoxin |
| title | Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique |
| title_full | Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique |
| title_fullStr | Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique |
| title_full_unstemmed | Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique |
| title_short | Detection of heat-labile toxin in enterotoxigenic Escherichia coli using PCR-ELISA technique |
| title_sort | detection of heat labile toxin in enterotoxigenic escherichia coli using pcr elisa technique |
| topic | Enterotoxigenic Escherichia coli PCR-ELISA Heat-labile enterotoxin |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-852&slc_lang=en&sid=1 |
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