Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto
Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become more widespread all over the world and it is important to determine methicillin resistance genes in different regions. The major goals of this work were to identify the mec-A gene related with MRSA and to assess the antibiogra...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zagazig University, Faculty of Medicine
2023-08-01
|
| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_253360_1f71101da9c961ec27194271edcb1d29.pdf |
| _version_ | 1797744819613728768 |
|---|---|
| author | Isah Musa Murtala Saadu Fatima Jibril |
| author_facet | Isah Musa Murtala Saadu Fatima Jibril |
| author_sort | Isah Musa |
| collection | DOAJ |
| description | Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become more widespread all over the world and it is important to determine methicillin resistance genes in different regions. The major goals of this work were to identify the mec-A gene related with MRSA and to assess the antibiogram of clinical isolates of S. aureus. Methods: Using normal microbiological techniques, 30 clinical Staphylococcal isolates from various specimens were processed to isolate S. aureus. The antibiotic susceptibility test was completed using the Kirby-Bauer disc-diffusion method in accordance with EUCAST criteria. Cefoxitin (30 g) discs were used to screen for MRSA isolates, and the standard polymerase chain reaction (PCR) was used to amplify the mec-A gene. Results: Staphylococcus aureus predominance was 66.6 percent (n = 20) among the 30 bacterial growths. Methicillin-resistant Staphylococcus aureus prevalence was 100% (n = 20), and multidrug resistance was present in 85% (17/20) of the cases (MDR). The majority of the S. aureus isolates were resistant to penicillin (95.2%), cefoxitin (100%), tigecycline (60%) and the combination antibiotics quinipristin-dalfopristin (50%) as well as tobramycin (30) and trimethoprim-methotrexate (20). The results of the PCR show that four out of the twelve isolates analyzed were mecA gene. Conclusion: Without taking antibiotic resistance into account and avoiding antibiotic use, fighting these superbugs won't be achievable. This might quickly escalate into an unmanageable situation. According to this study, MRSA is more common than previously believed and about 80% of isolates are multidrug resistant. |
| first_indexed | 2024-03-12T15:14:44Z |
| format | Article |
| id | doaj.art-1f4960e7da44498a9eabad8a5b88430e |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-03-12T15:14:44Z |
| publishDate | 2023-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-1f4960e7da44498a9eabad8a5b88430e2023-08-11T15:20:51ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402023-08-014380080810.21608/mid.2022.150608.1350253360Antibiogram and detection of mecA gene among MRSA at Specialist Hospital SokotoIsah Musa0Murtala Saadu1Fatima Jibril2Department of microbiology, Faculty of Life Sciences Kebbi state university of science and technology AleiroDepartment of Microbiology, Faculty of Life Sciences Kebbi State University of Science and Technology AlieroDepartment of Microbiology, Faculty of Life sciences, Kebbi State University of Science and Technology AlieroBackground: Methicillin-resistant Staphylococcus aureus (MRSA) has become more widespread all over the world and it is important to determine methicillin resistance genes in different regions. The major goals of this work were to identify the mec-A gene related with MRSA and to assess the antibiogram of clinical isolates of S. aureus. Methods: Using normal microbiological techniques, 30 clinical Staphylococcal isolates from various specimens were processed to isolate S. aureus. The antibiotic susceptibility test was completed using the Kirby-Bauer disc-diffusion method in accordance with EUCAST criteria. Cefoxitin (30 g) discs were used to screen for MRSA isolates, and the standard polymerase chain reaction (PCR) was used to amplify the mec-A gene. Results: Staphylococcus aureus predominance was 66.6 percent (n = 20) among the 30 bacterial growths. Methicillin-resistant Staphylococcus aureus prevalence was 100% (n = 20), and multidrug resistance was present in 85% (17/20) of the cases (MDR). The majority of the S. aureus isolates were resistant to penicillin (95.2%), cefoxitin (100%), tigecycline (60%) and the combination antibiotics quinipristin-dalfopristin (50%) as well as tobramycin (30) and trimethoprim-methotrexate (20). The results of the PCR show that four out of the twelve isolates analyzed were mecA gene. Conclusion: Without taking antibiotic resistance into account and avoiding antibiotic use, fighting these superbugs won't be achievable. This might quickly escalate into an unmanageable situation. According to this study, MRSA is more common than previously believed and about 80% of isolates are multidrug resistant.https://mid.journals.ekb.eg/article_253360_1f71101da9c961ec27194271edcb1d29.pdfkey words: mecamrsamultidrugpolymerase chain reaction (pcr)staphylococcus aureus |
| spellingShingle | Isah Musa Murtala Saadu Fatima Jibril Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto Microbes and Infectious Diseases key words: meca mrsa multidrug polymerase chain reaction (pcr) staphylococcus aureus |
| title | Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto |
| title_full | Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto |
| title_fullStr | Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto |
| title_full_unstemmed | Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto |
| title_short | Antibiogram and detection of mecA gene among MRSA at Specialist Hospital Sokoto |
| title_sort | antibiogram and detection of meca gene among mrsa at specialist hospital sokoto |
| topic | key words: meca mrsa multidrug polymerase chain reaction (pcr) staphylococcus aureus |
| url | https://mid.journals.ekb.eg/article_253360_1f71101da9c961ec27194271edcb1d29.pdf |
| work_keys_str_mv | AT isahmusa antibiogramanddetectionofmecageneamongmrsaatspecialisthospitalsokoto AT murtalasaadu antibiogramanddetectionofmecageneamongmrsaatspecialisthospitalsokoto AT fatimajibril antibiogramanddetectionofmecageneamongmrsaatspecialisthospitalsokoto |