In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system
Abstract Current practices in synthesizing molecularly imprinted polymers face challenges—lengthy process, low-productivity, the need for expensive and sophisticated equipment, and they cannot be controlled in situ synthesis. Herein, we present a micro-reactor for in situ and continuously synthesizi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-40413-8 |
| _version_ | 1797557923875913728 |
|---|---|
| author | Özgecan Erdem Ismail Eş Yeşeren Saylan Maryam Atabay Murat Alp Gungen Kadriye Ölmez Adil Denizli Fatih Inci |
| author_facet | Özgecan Erdem Ismail Eş Yeşeren Saylan Maryam Atabay Murat Alp Gungen Kadriye Ölmez Adil Denizli Fatih Inci |
| author_sort | Özgecan Erdem |
| collection | DOAJ |
| description | Abstract Current practices in synthesizing molecularly imprinted polymers face challenges—lengthy process, low-productivity, the need for expensive and sophisticated equipment, and they cannot be controlled in situ synthesis. Herein, we present a micro-reactor for in situ and continuously synthesizing trillions of molecularly imprinted polymeric nanoparticles that contain molecular fingerprints of bovine serum albumin in a short period of time (5-30 min). Initially, we performed COMSOL simulation to analyze mixing efficiency with altering flow rates, and experimentally validated the platform for synthesizing nanoparticles with sizes ranging from 52-106 nm. Molecular interactions between monomers and protein were also examined by molecular docking and dynamics simulations. Afterwards, we benchmarked the micro-reactor parameters through dispersity and concentration of molecularly imprinted polymers using principal component analysis. Sensing assets of molecularly imprinted polymers were examined on a metamaterial sensor, resulting in 81% of precision with high selectivity (4.5 times), and three cycles of consecutive use. Overall, our micro-reactor stood out for its high productivity (48-288 times improvement in assay-time and 2 times improvement in reagent volume), enabling to produce 1.4-1.5 times more MIPs at one-single step, and continuous production compared to conventional strategy. |
| first_indexed | 2024-03-10T17:24:06Z |
| format | Article |
| id | doaj.art-1f49d2a4eced4bb79a046254615a6016 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:24:06Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1f49d2a4eced4bb79a046254615a60162023-11-20T10:14:15ZengNature PortfolioNature Communications2041-17232023-08-0114111610.1038/s41467-023-40413-8In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor systemÖzgecan Erdem0Ismail Eş1Yeşeren Saylan2Maryam Atabay3Murat Alp Gungen4Kadriye Ölmez5Adil Denizli6Fatih Inci7UNAM-National Nanotechnology Research Center, Bilkent UniversityUNAM-National Nanotechnology Research Center, Bilkent UniversityDepartment of Chemistry, Hacettepe UniversityUNAM-National Nanotechnology Research Center, Bilkent UniversityUNAM-National Nanotechnology Research Center, Bilkent UniversityUNAM-National Nanotechnology Research Center, Bilkent UniversityDepartment of Chemistry, Hacettepe UniversityUNAM-National Nanotechnology Research Center, Bilkent UniversityAbstract Current practices in synthesizing molecularly imprinted polymers face challenges—lengthy process, low-productivity, the need for expensive and sophisticated equipment, and they cannot be controlled in situ synthesis. Herein, we present a micro-reactor for in situ and continuously synthesizing trillions of molecularly imprinted polymeric nanoparticles that contain molecular fingerprints of bovine serum albumin in a short period of time (5-30 min). Initially, we performed COMSOL simulation to analyze mixing efficiency with altering flow rates, and experimentally validated the platform for synthesizing nanoparticles with sizes ranging from 52-106 nm. Molecular interactions between monomers and protein were also examined by molecular docking and dynamics simulations. Afterwards, we benchmarked the micro-reactor parameters through dispersity and concentration of molecularly imprinted polymers using principal component analysis. Sensing assets of molecularly imprinted polymers were examined on a metamaterial sensor, resulting in 81% of precision with high selectivity (4.5 times), and three cycles of consecutive use. Overall, our micro-reactor stood out for its high productivity (48-288 times improvement in assay-time and 2 times improvement in reagent volume), enabling to produce 1.4-1.5 times more MIPs at one-single step, and continuous production compared to conventional strategy.https://doi.org/10.1038/s41467-023-40413-8 |
| spellingShingle | Özgecan Erdem Ismail Eş Yeşeren Saylan Maryam Atabay Murat Alp Gungen Kadriye Ölmez Adil Denizli Fatih Inci In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system Nature Communications |
| title | In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system |
| title_full | In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system |
| title_fullStr | In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system |
| title_full_unstemmed | In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system |
| title_short | In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system |
| title_sort | in situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro reactor system |
| url | https://doi.org/10.1038/s41467-023-40413-8 |
| work_keys_str_mv | AT ozgecanerdem insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT ismailes insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT yeserensaylan insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT maryamatabay insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT muratalpgungen insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT kadriyeolmez insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT adildenizli insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem AT fatihinci insitusynthesisanddynamicsimulationofmolecularlyimprintedpolymericnanoparticlesonamicroreactorsystem |