The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy
Aim: Neurodegeneration caused by the axonal injury is a widely seen phenomenon in spinal cord and traumatic brain injuries. Due to the disintegration of the synaptic connection neurotrophic factors could not be transported retrogradely towards the cell body and the deprivation of the trophic factors...
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| Format: | Article |
| Language: | English |
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Alanya Alaaddin Keykubat University
2019-08-01
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| Series: | Acta Medica Alanya |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/pub/medalanya/issue/48174/553846?publisher=alku |
| _version_ | 1797906434700083200 |
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| author | Mehmet Ozansoy Ebru Coşkun Muzaffer Beyza Çeti̇n Ozansoy Şeyda Çankaya Mehmet Yalçın Günal Zübeyir Bayraktaroğlu Burak Yuluğ Lütfü Hanoğlu |
| author_facet | Mehmet Ozansoy Ebru Coşkun Muzaffer Beyza Çeti̇n Ozansoy Şeyda Çankaya Mehmet Yalçın Günal Zübeyir Bayraktaroğlu Burak Yuluğ Lütfü Hanoğlu |
| author_sort | Mehmet Ozansoy |
| collection | DOAJ |
| description | Aim: Neurodegeneration caused by the axonal injury is a widely seen phenomenon in spinal cord and traumatic brain injuries. Due to the disintegration of the synaptic connection neurotrophic factors could not be transported retrogradely towards the cell body and the deprivation of the trophic factors lead to the degeneration and death of the injured neuron. Rifampicin is an antibiotic exhibiting several neuroprotective functions in various neurodegenerative conditions. Here we aim to investigate the acute neuroprotective effect of rifampicin in primary cortical neuron culture in which neurons are axotomized by laser axotomy.Methods: Neonatal male mice were used in order to isolate cortical neurons. Isolated primary cortical neurons were cultured. After 24 hours three different rifampicin concentrations (1 μM, 10 μM and 100 μM) were applied to the neurons and after 15 minutes of rifampicin addition, neurons were laser axotomized. Viability of the cells was evaluated by propidium iodide staining after 24 hours of axotomy. Results: Laser axotomy decreases the cortical neuron viability significantly by 80.45%, while rifampicin pre-treatment increases their viability in all three dosages in a statistically significant manner.
Conclusion: Rifampicin has an acute neuroprotective effect on the viability of the laser axotomized cortical neurons. |
| first_indexed | 2024-04-10T10:20:48Z |
| format | Article |
| id | doaj.art-1f4b38c9a24848ddac1556851997d983 |
| institution | Directory Open Access Journal |
| issn | 2587-0319 |
| language | English |
| last_indexed | 2024-04-10T10:20:48Z |
| publishDate | 2019-08-01 |
| publisher | Alanya Alaaddin Keykubat University |
| record_format | Article |
| series | Acta Medica Alanya |
| spelling | doaj.art-1f4b38c9a24848ddac1556851997d9832023-02-15T16:21:36ZengAlanya Alaaddin Keykubat UniversityActa Medica Alanya2587-03192019-08-013213514010.30565/medalanya.553846727The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomyMehmet Ozansoy0Ebru Coşkun1Muzaffer Beyza Çeti̇n Ozansoy2Şeyda Çankaya3Mehmet Yalçın Günal4Zübeyir Bayraktaroğlu5Burak Yuluğ6Lütfü Hanoğlu7İSTANBUL MEDİPOL ÜNİVERSİTESİİSTANBUL MEDİPOL ÜNİVERSİTESİİSTANBUL MEDİPOL ÜNİVERSİTESİALANYA ALAADDİN KEYKUBAT ÜNİVERSİTESİALANYA ALAADDİN KEYKUBAT ÜNİVERSİTESİİSTANBUL MEDİPOL ÜNİVERSİTESİALANYA ALAADDİN KEYKUBAT ÜNİVERSİTESİİSTANBUL MEDİPOL ÜNİVERSİTESİAim: Neurodegeneration caused by the axonal injury is a widely seen phenomenon in spinal cord and traumatic brain injuries. Due to the disintegration of the synaptic connection neurotrophic factors could not be transported retrogradely towards the cell body and the deprivation of the trophic factors lead to the degeneration and death of the injured neuron. Rifampicin is an antibiotic exhibiting several neuroprotective functions in various neurodegenerative conditions. Here we aim to investigate the acute neuroprotective effect of rifampicin in primary cortical neuron culture in which neurons are axotomized by laser axotomy.Methods: Neonatal male mice were used in order to isolate cortical neurons. Isolated primary cortical neurons were cultured. After 24 hours three different rifampicin concentrations (1 μM, 10 μM and 100 μM) were applied to the neurons and after 15 minutes of rifampicin addition, neurons were laser axotomized. Viability of the cells was evaluated by propidium iodide staining after 24 hours of axotomy. Results: Laser axotomy decreases the cortical neuron viability significantly by 80.45%, while rifampicin pre-treatment increases their viability in all three dosages in a statistically significant manner. Conclusion: Rifampicin has an acute neuroprotective effect on the viability of the laser axotomized cortical neurons.https://dergipark.org.tr/en/pub/medalanya/issue/48174/553846?publisher=alkulaser axotomyrifampicinneuronal viabilityprimary cortical neuronneurotraumalazer aksotomirifampisinnöronal canlılıkprimer kortikal nöronnörotravma |
| spellingShingle | Mehmet Ozansoy Ebru Coşkun Muzaffer Beyza Çeti̇n Ozansoy Şeyda Çankaya Mehmet Yalçın Günal Zübeyir Bayraktaroğlu Burak Yuluğ Lütfü Hanoğlu The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy Acta Medica Alanya laser axotomy rifampicin neuronal viability primary cortical neuron neurotrauma lazer aksotomi rifampisin nöronal canlılık primer kortikal nöron nörotravma |
| title | The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy |
| title_full | The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy |
| title_fullStr | The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy |
| title_full_unstemmed | The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy |
| title_short | The effect of rifampicin on the neuronal cell survival in prımary cortical neuron culture after laser axotomy |
| title_sort | effect of rifampicin on the neuronal cell survival in primary cortical neuron culture after laser axotomy |
| topic | laser axotomy rifampicin neuronal viability primary cortical neuron neurotrauma lazer aksotomi rifampisin nöronal canlılık primer kortikal nöron nörotravma |
| url | https://dergipark.org.tr/en/pub/medalanya/issue/48174/553846?publisher=alku |
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