Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study
Abstract Introduction Lung microbiome dysbiosis affects the immune system balance and promotes lung inflammation. We aimed to characterize and compare the lung bacteriome composition and the cytokine profile in women with normal lung function exposed to risk factors for chronic lung diseases (tobacc...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-04-01
|
| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.825 |
| _version_ | 1797830897819451392 |
|---|---|
| author | Fernando Morales‐González Juan A. Lira‐Lucio Ramcés Falfán‐Valencia José E. Márquez‐García Edgar Abarca‐Rojano Alejandra Ramírez‐Venegas Raúl H. Sansores Leonor García‐Gómez Andrea Hernández‐Pérez Gloria Pérez‐Rubio |
| author_facet | Fernando Morales‐González Juan A. Lira‐Lucio Ramcés Falfán‐Valencia José E. Márquez‐García Edgar Abarca‐Rojano Alejandra Ramírez‐Venegas Raúl H. Sansores Leonor García‐Gómez Andrea Hernández‐Pérez Gloria Pérez‐Rubio |
| author_sort | Fernando Morales‐González |
| collection | DOAJ |
| description | Abstract Introduction Lung microbiome dysbiosis affects the immune system balance and promotes lung inflammation. We aimed to characterize and compare the lung bacteriome composition and the cytokine profile in women with normal lung function exposed to risk factors for chronic lung diseases (tobacco smoking and biomass‐burning smoke exposure). Methods We included women with biomass‐burning smoke exposure (BE, n = 11) and current smokers women (TS, n = 10). The bacteriome composition was performed in induced sputum, sequencing the 16 rRNA gene. Cytokine levels were measured using enzyme‐linked immunosorbent assay multiplex assay in the supernatant of induced sputum. For quantitative variables, we used medians and minimum and maxim values. For the amplicon sequence variants (ASV) differential abundance testing between groups. Results At the taxa level, the phylum Proteobacteria was found in a higher proportion in the TS group concerning BE (p = .045); however, after the false discovery rate adjustment, this difference was not retained (p = .288). We found a higher concentration of IL‐1β in the TS group than in the BE group (248.6 vs. 177.9 pg/mL, p = .010). Women with high biomass‐burning smoke exposure in an hour per day had a positive correlation with the abundance of Bacteroidota (ρ = 0.71, p = .014) and Fusobacteriota (ρ = 0.73, p = .011). FEV1/FVC had a positive correlation with an abundance of Bacteroidota, Proteobacteria, and Fusobacteria (ρ = 0.74, p = .009, ρ = 0.85, p = .001, and ρ = 0.83, p = .001, respectively). In tobacco smoking, women had a positive correlation (ρ = 0.77, p = .009) between cigarettes per day and Firmicutes' abundance. Conclusion Compared to biomass‐burning smoke‐exposed women, current smokers have poor lung function and high levels of IL‐1β in sputum. Women with biomass‐burning smoke exposure present an increased abundance of Bacteroidota and Fusobacteriota. |
| first_indexed | 2024-04-09T13:43:42Z |
| format | Article |
| id | doaj.art-1f4b806802f542beb0d3fda613d95ff8 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-04-09T13:43:42Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-1f4b806802f542beb0d3fda613d95ff82023-05-09T06:57:15ZengWileyImmunity, Inflammation and Disease2050-45272023-04-01114n/an/a10.1002/iid3.825Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot studyFernando Morales‐González0Juan A. Lira‐Lucio1Ramcés Falfán‐Valencia2José E. Márquez‐García3Edgar Abarca‐Rojano4Alejandra Ramírez‐Venegas5Raúl H. Sansores6Leonor García‐Gómez7Andrea Hernández‐Pérez8Gloria Pérez‐Rubio9HLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoSubdirección de Investigación Biomédica, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional Mexico City MexicoDepartment of Tobacco Smoking and COPD Research Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoClínica de Enfermedades Respiratorias, Fundación Médica Sur Mexico City MexicoDepartment of Tobacco Smoking and COPD Research Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoDepartment of Tobacco Smoking and COPD Research Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoHLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas Mexico City MexicoAbstract Introduction Lung microbiome dysbiosis affects the immune system balance and promotes lung inflammation. We aimed to characterize and compare the lung bacteriome composition and the cytokine profile in women with normal lung function exposed to risk factors for chronic lung diseases (tobacco smoking and biomass‐burning smoke exposure). Methods We included women with biomass‐burning smoke exposure (BE, n = 11) and current smokers women (TS, n = 10). The bacteriome composition was performed in induced sputum, sequencing the 16 rRNA gene. Cytokine levels were measured using enzyme‐linked immunosorbent assay multiplex assay in the supernatant of induced sputum. For quantitative variables, we used medians and minimum and maxim values. For the amplicon sequence variants (ASV) differential abundance testing between groups. Results At the taxa level, the phylum Proteobacteria was found in a higher proportion in the TS group concerning BE (p = .045); however, after the false discovery rate adjustment, this difference was not retained (p = .288). We found a higher concentration of IL‐1β in the TS group than in the BE group (248.6 vs. 177.9 pg/mL, p = .010). Women with high biomass‐burning smoke exposure in an hour per day had a positive correlation with the abundance of Bacteroidota (ρ = 0.71, p = .014) and Fusobacteriota (ρ = 0.73, p = .011). FEV1/FVC had a positive correlation with an abundance of Bacteroidota, Proteobacteria, and Fusobacteria (ρ = 0.74, p = .009, ρ = 0.85, p = .001, and ρ = 0.83, p = .001, respectively). In tobacco smoking, women had a positive correlation (ρ = 0.77, p = .009) between cigarettes per day and Firmicutes' abundance. Conclusion Compared to biomass‐burning smoke‐exposed women, current smokers have poor lung function and high levels of IL‐1β in sputum. Women with biomass‐burning smoke exposure present an increased abundance of Bacteroidota and Fusobacteriota.https://doi.org/10.1002/iid3.825dysbiosisimmunityinflammationrespiratory diseaserespiratory microbiome |
| spellingShingle | Fernando Morales‐González Juan A. Lira‐Lucio Ramcés Falfán‐Valencia José E. Márquez‐García Edgar Abarca‐Rojano Alejandra Ramírez‐Venegas Raúl H. Sansores Leonor García‐Gómez Andrea Hernández‐Pérez Gloria Pérez‐Rubio Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study Immunity, Inflammation and Disease dysbiosis immunity inflammation respiratory disease respiratory microbiome |
| title | Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study |
| title_full | Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study |
| title_fullStr | Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study |
| title_full_unstemmed | Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study |
| title_short | Characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors: A pilot study |
| title_sort | characterization of the lung microbiome and inflammatory cytokine levels in women exposed to environmental risk factors a pilot study |
| topic | dysbiosis immunity inflammation respiratory disease respiratory microbiome |
| url | https://doi.org/10.1002/iid3.825 |
| work_keys_str_mv | AT fernandomoralesgonzalez characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT juanaliralucio characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT ramcesfalfanvalencia characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT joseemarquezgarcia characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT edgarabarcarojano characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT alejandraramirezvenegas characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT raulhsansores characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT leonorgarciagomez characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT andreahernandezperez characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy AT gloriaperezrubio characterizationofthelungmicrobiomeandinflammatorycytokinelevelsinwomenexposedtoenvironmentalriskfactorsapilotstudy |