In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots
<p>Abstract</p> <p>Background</p> <p>Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms, the tissue and cell specific pattern of expressi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-11-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/9/133 |
| _version_ | 1818564961835679744 |
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| author | Khonsari Roman H Hunter Paul R Sorieul Mathias Gattolin Stefano Frigerio Lorenzo |
| author_facet | Khonsari Roman H Hunter Paul R Sorieul Mathias Gattolin Stefano Frigerio Lorenzo |
| author_sort | Khonsari Roman H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms, the tissue and cell specific pattern of expression are not known.</p> <p>Results</p> <p>We generated fluorescent protein fusions to the genomic sequences of all members of the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2; TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP distribution varied between different cell layers within the root axis, with extensive co-expression of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs and smaller, uncharacterised structures.</p> <p>Conclusion</p> <p>We have produced a comprehensive atlas of TIP expression in Arabidopsis roots, which reveals novel expression patterns for not previously studied TIPs.</p> |
| first_indexed | 2024-12-14T01:35:14Z |
| format | Article |
| id | doaj.art-1f4bfc36bc134e31b2ec714da244b6c5 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-14T01:35:14Z |
| publishDate | 2009-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-1f4bfc36bc134e31b2ec714da244b6c52022-12-21T23:21:53ZengBMCBMC Plant Biology1471-22292009-11-019113310.1186/1471-2229-9-133In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis rootsKhonsari Roman HHunter Paul RSorieul MathiasGattolin StefanoFrigerio Lorenzo<p>Abstract</p> <p>Background</p> <p>Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms, the tissue and cell specific pattern of expression are not known.</p> <p>Results</p> <p>We generated fluorescent protein fusions to the genomic sequences of all members of the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2; TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP distribution varied between different cell layers within the root axis, with extensive co-expression of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs and smaller, uncharacterised structures.</p> <p>Conclusion</p> <p>We have produced a comprehensive atlas of TIP expression in Arabidopsis roots, which reveals novel expression patterns for not previously studied TIPs.</p>http://www.biomedcentral.com/1471-2229/9/133 |
| spellingShingle | Khonsari Roman H Hunter Paul R Sorieul Mathias Gattolin Stefano Frigerio Lorenzo In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots BMC Plant Biology |
| title | In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots |
| title_full | In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots |
| title_fullStr | In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots |
| title_full_unstemmed | In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots |
| title_short | In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots |
| title_sort | in vivo imaging of the tonoplast intrinsic protein family in arabidopsis roots |
| url | http://www.biomedcentral.com/1471-2229/9/133 |
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