On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells
Microtubules are dynamic structures undergoing rapid growth and shrinkage in living cells and in vitro. The growth of microtubules in vitro was analyzed with subpixel precision (Maurer et al., Current Biology, 2014, 24 (4), 372–384); however, to what extent these results could be applied for microtu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.745089/full |
| _version_ | 1818913984081821696 |
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| author | Arshat Urazbaev Anara Serikbaeva Anara Serikbaeva Anna Tvorogova Azamat Dusenbayev Sholpan Kauanova Sholpan Kauanova Ivan Vorobjev Ivan Vorobjev Ivan Vorobjev |
| author_facet | Arshat Urazbaev Anara Serikbaeva Anara Serikbaeva Anna Tvorogova Azamat Dusenbayev Sholpan Kauanova Sholpan Kauanova Ivan Vorobjev Ivan Vorobjev Ivan Vorobjev |
| author_sort | Arshat Urazbaev |
| collection | DOAJ |
| description | Microtubules are dynamic structures undergoing rapid growth and shrinkage in living cells and in vitro. The growth of microtubules in vitro was analyzed with subpixel precision (Maurer et al., Current Biology, 2014, 24 (4), 372–384); however, to what extent these results could be applied for microtubules growing in vivo remains largely unknown. Particularly, the question is whether microtubule growth velocity in cells could be sufficiently approximated by a Gaussian distribution or its variability requires a more sophisticated description? Addressing this question, we used time-lapse microscopy and mathematical modeling, and we analyzed EB-3 comets forming on microtubules of cultured cells with subpixel precision. Parameters of comets (shape, form, and velocity) were used as topological characteristics of 3D voxel objects. Using regression analysis, we determined the real positions of the microtubule tips in time-lapse sequences. By exponential decay fitting of the restored comet intensity profile, we found that in vivo EB-3 rapidly exchanges on growing microtubule ends with a decoration time ∼ 2 s. We next developed the model showing that the best correlation between comet length and microtubule end growth velocity is at time intervals close to the decoration time. In the cells, EB comet length positively correlates with microtubule growth velocity in preceding time intervals, while demonstrating no correlation in subsequent time intervals. Correlation between comet length and instantaneous growth velocity of microtubules remains under nocodazole treatment when mean values of both parameters decrease. Our data show that the growth of microtubules in living cells is well-approximated by a constant velocity with large stochastic fluctuations. |
| first_indexed | 2024-12-19T23:39:10Z |
| format | Article |
| id | doaj.art-1f4d48a579ea41f4b40530488c920c1f |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-19T23:39:10Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-1f4d48a579ea41f4b40530488c920c1f2022-12-21T20:01:29ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-11-01810.3389/fmolb.2021.745089745089On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured CellsArshat Urazbaev0Anara Serikbaeva1Anara Serikbaeva2Anna Tvorogova3Azamat Dusenbayev4Sholpan Kauanova5Sholpan Kauanova6Ivan Vorobjev7Ivan Vorobjev8Ivan Vorobjev9National Laboratory Astana, Nazarbayev University, Nur-Sultan, KazakhstanLaboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, KazakhstanDepartment of Physiology and Biophysics (M/C 901), University of Illinois at Chicago, Chicago, IL, United StatesA.N.Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaNational Laboratory Astana, Nazarbayev University, Nur-Sultan, KazakhstanLaboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, KazakhstanDepartment of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, KazakhstanLaboratory of Biophotonics and Imaging, National Laboratory Astana, Nazarbayev University, Nur-Sultan, KazakhstanA.N.Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, KazakhstanMicrotubules are dynamic structures undergoing rapid growth and shrinkage in living cells and in vitro. The growth of microtubules in vitro was analyzed with subpixel precision (Maurer et al., Current Biology, 2014, 24 (4), 372–384); however, to what extent these results could be applied for microtubules growing in vivo remains largely unknown. Particularly, the question is whether microtubule growth velocity in cells could be sufficiently approximated by a Gaussian distribution or its variability requires a more sophisticated description? Addressing this question, we used time-lapse microscopy and mathematical modeling, and we analyzed EB-3 comets forming on microtubules of cultured cells with subpixel precision. Parameters of comets (shape, form, and velocity) were used as topological characteristics of 3D voxel objects. Using regression analysis, we determined the real positions of the microtubule tips in time-lapse sequences. By exponential decay fitting of the restored comet intensity profile, we found that in vivo EB-3 rapidly exchanges on growing microtubule ends with a decoration time ∼ 2 s. We next developed the model showing that the best correlation between comet length and microtubule end growth velocity is at time intervals close to the decoration time. In the cells, EB comet length positively correlates with microtubule growth velocity in preceding time intervals, while demonstrating no correlation in subsequent time intervals. Correlation between comet length and instantaneous growth velocity of microtubules remains under nocodazole treatment when mean values of both parameters decrease. Our data show that the growth of microtubules in living cells is well-approximated by a constant velocity with large stochastic fluctuations.https://www.frontiersin.org/articles/10.3389/fmolb.2021.745089/fullmicrotubulesdynamic instabilityEB proteinsfluorescent microscopycell culturelive cell imaging |
| spellingShingle | Arshat Urazbaev Anara Serikbaeva Anara Serikbaeva Anna Tvorogova Azamat Dusenbayev Sholpan Kauanova Sholpan Kauanova Ivan Vorobjev Ivan Vorobjev Ivan Vorobjev On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells Frontiers in Molecular Biosciences microtubules dynamic instability EB proteins fluorescent microscopy cell culture live cell imaging |
| title | On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells |
| title_full | On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells |
| title_fullStr | On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells |
| title_full_unstemmed | On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells |
| title_short | On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells |
| title_sort | on the relationship between eb 3 profiles and microtubules growth in cultured cells |
| topic | microtubules dynamic instability EB proteins fluorescent microscopy cell culture live cell imaging |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.745089/full |
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