Purification and Characterization of Streptomyces sp. SKK1-8 xylanase
Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2008-11-01
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| Series: | Makara Seri Sains |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/science/article/viewFile/336/332 |
| _version_ | 1818612951551049728 |
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| author | Nunuk Widhyastuti Yulin Lestari Anja Meryandini |
| author_facet | Nunuk Widhyastuti Yulin Lestari Anja Meryandini |
| author_sort | Nunuk Widhyastuti |
| collection | DOAJ |
| description | Streptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50oC measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml. |
| first_indexed | 2024-12-16T15:54:23Z |
| format | Article |
| id | doaj.art-1f4da122caf94d5fb19b46c16d6ccd26 |
| institution | Directory Open Access Journal |
| issn | 1693-6671 |
| language | English |
| last_indexed | 2024-12-16T15:54:23Z |
| publishDate | 2008-11-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Seri Sains |
| spelling | doaj.art-1f4da122caf94d5fb19b46c16d6ccd262022-12-21T22:25:37ZengUniversitas IndonesiaMakara Seri Sains1693-66712008-11-011225560Purification and Characterization of Streptomyces sp. SKK1-8 xylanaseNunuk WidhyastutiYulin LestariAnja MeryandiniStreptomyces sp. SKK1-8 is a xylanase produced bacteria. Purified xylanase has an optimum condition at pH 4.5 and 50oC. The molecular mass of purified xylanase were determined to be 14.4 kDa and 13.4 kDa. The xylanase was capable of hydrolysing p-NP-α-L-arabinofuranoside, p-NP-β-D-xylanopiranoside, p-NP-β-D-glucopiranoside, p-NP-α-D-galactopiranoside. The Km and Vmax values at 50oC measured on Birchwood xylan were 0.101 mg/ml and 0.1796 μmoles/minute/ml.http://journal.ui.ac.id/science/article/viewFile/336/332Xylanasestreptomycespurified |
| spellingShingle | Nunuk Widhyastuti Yulin Lestari Anja Meryandini Purification and Characterization of Streptomyces sp. SKK1-8 xylanase Makara Seri Sains Xylanase streptomyces purified |
| title | Purification and Characterization of Streptomyces sp. SKK1-8 xylanase |
| title_full | Purification and Characterization of Streptomyces sp. SKK1-8 xylanase |
| title_fullStr | Purification and Characterization of Streptomyces sp. SKK1-8 xylanase |
| title_full_unstemmed | Purification and Characterization of Streptomyces sp. SKK1-8 xylanase |
| title_short | Purification and Characterization of Streptomyces sp. SKK1-8 xylanase |
| title_sort | purification and characterization of streptomyces sp skk1 8 xylanase |
| topic | Xylanase streptomyces purified |
| url | http://journal.ui.ac.id/science/article/viewFile/336/332 |
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