Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens
The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is high under catabolic conditions that result in muscle atrophy. We previously showed that expression of atrogin-1/MAFbx was expressed in skeletal, smooth and cardiac muscles of chickens and its expression in skeletal and sm...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2015-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/52/4/52_0150036/_pdf/-char/en |
| _version_ | 1797816920453414912 |
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| author | Kazuki Nakashima Aiko Ishida |
| author_facet | Kazuki Nakashima Aiko Ishida |
| author_sort | Kazuki Nakashima |
| collection | DOAJ |
| description | The expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is high under catabolic conditions that result in muscle atrophy. We previously showed that expression of atrogin-1/MAFbx was expressed in skeletal, smooth and cardiac muscles of chickens and its expression in skeletal and smooth muscles was regulated by nutritional conditions. In this study, the effects of fasting and refeeding on the expression of atrogin-1/MAFbx and other proteolytic-related genes, including 20S proteasome C2 subunit, m-calpain large subunit, and cathepsin B, in cardiac muscle of broiler chickens were investigated. Chickens were fasted for 24 h, and refed for 2 h. Atrogin-1/MAFbx mRNA expression was increased by fasting, but subsequent refeeding reduced the increase. The mRNA expression of the 20S proteasome C2 subunit and m-calpain large subunit mRNA was not affected by fasting or refeeding. Cathepsin B mRNA expression was increased by fasting, but unlike atrogin-1/MAFbx mRNA expression, the increase was not reduced by refeeding. These results demonstrate the muscle-specific ubiquitin ligase atrogin-1/MAFbx gene expression in cardiac muscle as well as skeletal and smooth muscles of chicken is regulated by food deprivation and nutritional supply. |
| first_indexed | 2024-03-13T08:45:48Z |
| format | Article |
| id | doaj.art-1f50716dfa5045ef9ff1cd5e4c9437be |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:48Z |
| publishDate | 2015-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-1f50716dfa5045ef9ff1cd5e4c9437be2023-05-30T04:29:43ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862015-10-0152431832210.2141/jpsa.0150036jpsaEffects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler ChickensKazuki Nakashima0Aiko Ishida1Animal Physiology and Nutrition Division, NARO Institute of Livestock and Grassland Science, JapanAnimal Physiology and Nutrition Division, NARO Institute of Livestock and Grassland Science, JapanThe expression of atrogin-1/MAFbx, a muscle-specific E3 ubiquitin ligase, is high under catabolic conditions that result in muscle atrophy. We previously showed that expression of atrogin-1/MAFbx was expressed in skeletal, smooth and cardiac muscles of chickens and its expression in skeletal and smooth muscles was regulated by nutritional conditions. In this study, the effects of fasting and refeeding on the expression of atrogin-1/MAFbx and other proteolytic-related genes, including 20S proteasome C2 subunit, m-calpain large subunit, and cathepsin B, in cardiac muscle of broiler chickens were investigated. Chickens were fasted for 24 h, and refed for 2 h. Atrogin-1/MAFbx mRNA expression was increased by fasting, but subsequent refeeding reduced the increase. The mRNA expression of the 20S proteasome C2 subunit and m-calpain large subunit mRNA was not affected by fasting or refeeding. Cathepsin B mRNA expression was increased by fasting, but unlike atrogin-1/MAFbx mRNA expression, the increase was not reduced by refeeding. These results demonstrate the muscle-specific ubiquitin ligase atrogin-1/MAFbx gene expression in cardiac muscle as well as skeletal and smooth muscles of chicken is regulated by food deprivation and nutritional supply.https://www.jstage.jst.go.jp/article/jpsa/52/4/52_0150036/_pdf/-char/enatrogin-1/mafbxbroilercardiac musclefastingheartrefeeding |
| spellingShingle | Kazuki Nakashima Aiko Ishida Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens The Journal of Poultry Science atrogin-1/mafbx broiler cardiac muscle fasting heart refeeding |
| title | Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens |
| title_full | Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens |
| title_fullStr | Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens |
| title_full_unstemmed | Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens |
| title_short | Effects of Fasting and Refeeding on Expression of Atrogin-1/MAFbx in Cardiac Muscle of Broiler Chickens |
| title_sort | effects of fasting and refeeding on expression of atrogin 1 mafbx in cardiac muscle of broiler chickens |
| topic | atrogin-1/mafbx broiler cardiac muscle fasting heart refeeding |
| url | https://www.jstage.jst.go.jp/article/jpsa/52/4/52_0150036/_pdf/-char/en |
| work_keys_str_mv | AT kazukinakashima effectsoffastingandrefeedingonexpressionofatrogin1mafbxincardiacmuscleofbroilerchickens AT aikoishida effectsoffastingandrefeedingonexpressionofatrogin1mafbxincardiacmuscleofbroilerchickens |