Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut
In the past decade, mycotoxin zearalenone (ZEN)-induced gastrointestinal adverse effects have been increasingly attracting worldwide attention. This study aimed to determine the gastrointestinal adverse effects of ZEN in Drosophila melanogaster (D. melanogaster) and reveal possible mechanisms of act...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323000593 |
| _version_ | 1797936431764602880 |
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| author | Zhi Chen Fen Wang Wen Zhang Shuangshuang Zhou Di Wen Ren Mu |
| author_facet | Zhi Chen Fen Wang Wen Zhang Shuangshuang Zhou Di Wen Ren Mu |
| author_sort | Zhi Chen |
| collection | DOAJ |
| description | In the past decade, mycotoxin zearalenone (ZEN)-induced gastrointestinal adverse effects have been increasingly attracting worldwide attention. This study aimed to determine the gastrointestinal adverse effects of ZEN in Drosophila melanogaster (D. melanogaster) and reveal possible mechanisms of action of ZEN in insects. Here, chronic exposure of D. melanogaster to ZEN killed flies in a dose-dependent manner (2–20 µM). ZEN (20 µM) decreased the survival rates and climbing ability of flies, and activated immune deficiency-mediated intestinal immunity in midgut, leading to the production of antimicrobial peptides. Meanwhile, ZEN exposure induced morphological alteration of adult midgut. Further study suggested that high levels of oxidative stress was observed in ZEN-treated midgut due to the imbalance between the production of reactive oxygen species and the expression and activities of cellular antioxidant enzyme, including superoxide dismutase and catalase. ZEN-induced oxidative stress then caused cell death, impaired gut barrier function and increased gut permeability, leading to oxidative injury in midgut. Subsequently, ZEN-induce midgut injury further disrupted intestinal stem cell (ISC) homeostasis, stimulating ISC proliferation and tissue regeneration, but did not alter cell fate specification of ISC. Additionally, activation of Jun N-terminal kinase pathway was involved in ZEN-induced oxidative injury and tissue regeneration in midgut. Antioxidant vitamin E alleviated ZEN-induced oxidative injury to midgut epithelium. Collectively, this study provided additional evidences for ZEN-induced gastrointestinal adverse effects from an invertebrate model, extended our understanding of the mechanisms mediating mycotoxin toxicity in organisms. |
| first_indexed | 2024-04-10T18:29:35Z |
| format | Article |
| id | doaj.art-1f52962f5085470b9fe4a904adbd20d2 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T18:29:35Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-1f52962f5085470b9fe4a904adbd20d22023-02-02T04:47:06ZengElsevierEcotoxicology and Environmental Safety0147-65132023-02-01251114555Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgutZhi Chen0Fen Wang1Wen Zhang2Shuangshuang Zhou3Di Wen4Ren Mu5Corresponding authors.; College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCorresponding authors.; College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCorresponding authors.; College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaIn the past decade, mycotoxin zearalenone (ZEN)-induced gastrointestinal adverse effects have been increasingly attracting worldwide attention. This study aimed to determine the gastrointestinal adverse effects of ZEN in Drosophila melanogaster (D. melanogaster) and reveal possible mechanisms of action of ZEN in insects. Here, chronic exposure of D. melanogaster to ZEN killed flies in a dose-dependent manner (2–20 µM). ZEN (20 µM) decreased the survival rates and climbing ability of flies, and activated immune deficiency-mediated intestinal immunity in midgut, leading to the production of antimicrobial peptides. Meanwhile, ZEN exposure induced morphological alteration of adult midgut. Further study suggested that high levels of oxidative stress was observed in ZEN-treated midgut due to the imbalance between the production of reactive oxygen species and the expression and activities of cellular antioxidant enzyme, including superoxide dismutase and catalase. ZEN-induced oxidative stress then caused cell death, impaired gut barrier function and increased gut permeability, leading to oxidative injury in midgut. Subsequently, ZEN-induce midgut injury further disrupted intestinal stem cell (ISC) homeostasis, stimulating ISC proliferation and tissue regeneration, but did not alter cell fate specification of ISC. Additionally, activation of Jun N-terminal kinase pathway was involved in ZEN-induced oxidative injury and tissue regeneration in midgut. Antioxidant vitamin E alleviated ZEN-induced oxidative injury to midgut epithelium. Collectively, this study provided additional evidences for ZEN-induced gastrointestinal adverse effects from an invertebrate model, extended our understanding of the mechanisms mediating mycotoxin toxicity in organisms.http://www.sciencedirect.com/science/article/pii/S0147651323000593ZearalenoneDrosophila midgutIntestinal immunityOxidative stressCell deathTissue regeneration |
| spellingShingle | Zhi Chen Fen Wang Wen Zhang Shuangshuang Zhou Di Wen Ren Mu Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut Ecotoxicology and Environmental Safety Zearalenone Drosophila midgut Intestinal immunity Oxidative stress Cell death Tissue regeneration |
| title | Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut |
| title_full | Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut |
| title_fullStr | Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut |
| title_full_unstemmed | Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut |
| title_short | Chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut |
| title_sort | chronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult drosophila melanogaster midgut |
| topic | Zearalenone Drosophila midgut Intestinal immunity Oxidative stress Cell death Tissue regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323000593 |
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