Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i>
Vincristine is an anti-cancer compound and one of the most crucial vinca alkaloids produced by the medicinal plant <i>Catharanthus roseus</i> (L.) G. Don. (Apocynaceae). This plant is home to hundreds of endophytic microbes, which produce a variety of bioactive secondary metabolites that...
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MDPI AG
2022-11-01
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| Series: | Metabolites |
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| author | Kanchan Birat Reem Binsuwaidan Tariq Omar Siddiqi Showkat Rasool Mir Nawaf Alshammari Mohd Adnan Rahila Nazir Bushra Ejaz Moien Qadir Malik Rikeshwer Prasad Dewangan Syed Amir Ashraf Bibhu Prasad Panda |
| author_facet | Kanchan Birat Reem Binsuwaidan Tariq Omar Siddiqi Showkat Rasool Mir Nawaf Alshammari Mohd Adnan Rahila Nazir Bushra Ejaz Moien Qadir Malik Rikeshwer Prasad Dewangan Syed Amir Ashraf Bibhu Prasad Panda |
| author_sort | Kanchan Birat |
| collection | DOAJ |
| description | Vincristine is an anti-cancer compound and one of the most crucial vinca alkaloids produced by the medicinal plant <i>Catharanthus roseus</i> (L.) G. Don. (Apocynaceae). This plant is home to hundreds of endophytic microbes, which produce a variety of bioactive secondary metabolites that are known for their medicinal properties. In this study, we focused on isolating an endophytic fungus that could increase the yield of vincristine under laboratory conditions as an alternative to plant-mediated extraction of vincristine. The endophytic fungus <i>Nigrospora zimmermanii</i> (Apiosporaceae) was isolated from <i>Catharanthus roseus</i> and it was found to be producing the anticancer compound vincristine. It was identified using high-performance thin-layer chromatography by matching the Rf value and spectral data with the vincristine standard and mass spectrometry data and the reference molecule from the PubChem database. The generation study of this microbe showed that the production of vincristine in the parent fungus was at its maximum, i.e., 5.344 µg/mL, while it was slightly reduced in subsequent generations. A colonization study was also performed and it showed that the fungus <i>N. zimmermanii</i> was able to re-infect the plant <i>Catharanthus roseus</i> after 20 days of inoculation. The colonization study showed that <i>N. zimmernanii</i> could infect the plant after isolation. This method is an efficient and easy way to obtain a high yield of vincristine, as compared to plant-mediated production. |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T18:08:38Z |
| publishDate | 2022-11-01 |
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| series | Metabolites |
| spelling | doaj.art-1f52f5f98f53446db001453fe05a9a5b2023-11-24T09:13:20ZengMDPI AGMetabolites2218-19892022-11-011211111910.3390/metabo12111119Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i>Kanchan Birat0Reem Binsuwaidan1Tariq Omar Siddiqi2Showkat Rasool Mir3Nawaf Alshammari4Mohd Adnan5Rahila Nazir6Bushra Ejaz7Moien Qadir Malik8Rikeshwer Prasad Dewangan9Syed Amir Ashraf10Bibhu Prasad Panda11Microbial and Pharmaceutical Biotechnology Laboratory, Department of Pharmacognosy & Phytochemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh P.O. Box 84428, Saudi ArabiaMolecular Ecology Laboratory, Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaPhytopharmaceuticals Research Laboratory, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi ArabiaMolecular Ecology Laboratory, Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaMolecular Ecology Laboratory, Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaMolecular Ecology Laboratory, Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Pharmaceutical Chemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, Hail P.O. Box 2440, Saudi ArabiaMicrobial and Pharmaceutical Biotechnology Laboratory, Department of Pharmacognosy & Phytochemistry, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi 110062, IndiaVincristine is an anti-cancer compound and one of the most crucial vinca alkaloids produced by the medicinal plant <i>Catharanthus roseus</i> (L.) G. Don. (Apocynaceae). This plant is home to hundreds of endophytic microbes, which produce a variety of bioactive secondary metabolites that are known for their medicinal properties. In this study, we focused on isolating an endophytic fungus that could increase the yield of vincristine under laboratory conditions as an alternative to plant-mediated extraction of vincristine. The endophytic fungus <i>Nigrospora zimmermanii</i> (Apiosporaceae) was isolated from <i>Catharanthus roseus</i> and it was found to be producing the anticancer compound vincristine. It was identified using high-performance thin-layer chromatography by matching the Rf value and spectral data with the vincristine standard and mass spectrometry data and the reference molecule from the PubChem database. The generation study of this microbe showed that the production of vincristine in the parent fungus was at its maximum, i.e., 5.344 µg/mL, while it was slightly reduced in subsequent generations. A colonization study was also performed and it showed that the fungus <i>N. zimmermanii</i> was able to re-infect the plant <i>Catharanthus roseus</i> after 20 days of inoculation. The colonization study showed that <i>N. zimmernanii</i> could infect the plant after isolation. This method is an efficient and easy way to obtain a high yield of vincristine, as compared to plant-mediated production.https://www.mdpi.com/2218-1989/12/11/1119vincristine<i>Catharanthus roseus</i><i>Nigrospora zimmermanii</i>endophytevinca alkaloidsanticancer compound |
| spellingShingle | Kanchan Birat Reem Binsuwaidan Tariq Omar Siddiqi Showkat Rasool Mir Nawaf Alshammari Mohd Adnan Rahila Nazir Bushra Ejaz Moien Qadir Malik Rikeshwer Prasad Dewangan Syed Amir Ashraf Bibhu Prasad Panda Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> Metabolites vincristine <i>Catharanthus roseus</i> <i>Nigrospora zimmermanii</i> endophyte vinca alkaloids anticancer compound |
| title | Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> |
| title_full | Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> |
| title_fullStr | Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> |
| title_full_unstemmed | Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> |
| title_short | Report on Vincristine-Producing Endophytic Fungus <i>Nigrospora zimmermanii</i> from Leaves of <i>Catharanthus roseus</i> |
| title_sort | report on vincristine producing endophytic fungus i nigrospora zimmermanii i from leaves of i catharanthus roseus i |
| topic | vincristine <i>Catharanthus roseus</i> <i>Nigrospora zimmermanii</i> endophyte vinca alkaloids anticancer compound |
| url | https://www.mdpi.com/2218-1989/12/11/1119 |
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