Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results
Knee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observa...
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MDPI AG
2023-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/13/4330 |
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| author | Marco Turati Silvia Franchi Marco Crippa Laura Rizzi Luca Rigamonti Paola Sacerdote Simone Daniel Gatti Massimiliano Piatti Giulia Galimberti Daniele Munegato Giada Amodeo Robert J. Omeljaniuk Giovanni Zatti Antonio Torsello Marco Bigoni |
| author_facet | Marco Turati Silvia Franchi Marco Crippa Laura Rizzi Luca Rigamonti Paola Sacerdote Simone Daniel Gatti Massimiliano Piatti Giulia Galimberti Daniele Munegato Giada Amodeo Robert J. Omeljaniuk Giovanni Zatti Antonio Torsello Marco Bigoni |
| author_sort | Marco Turati |
| collection | DOAJ |
| description | Knee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observational study examined the potential involvement of PK2 in degenerative or traumatic knee disease. Fifteen patients presenting knee osteoarthritis (OA group) and 15 patients presenting a traumatic meniscal tear (TM group) were studied. Synovial fluid samples from affected knees were assessed for PK2, IL-10, and TNF-α using the ELISA method. At a long-term follow-up (minimum 5 years, mean = 6.1 years), patients in the TM group underwent clinical re-evaluation with PROMs (Tegner Activity Scale, IKDC, Lysholm, SKV); in addition, X-ray visualization was used to assess the presence of secondary OA. PK2 was detected in synovial fluids of both TM and OA patients and the levels were comparable between the two groups, while IL-10 levels were significantly greater in the OA group than those in TM patients. PK2 levels correlated with those of IL-10. PK2 levels were greater in blood effusions compared to clear samples, did not differ significantly between sexes, nor were they related to differences in weight, height, or injury (meniscal laterality, time since dosing). No correlation was found between PROMs and radiological classifications in patients in the TM group at final follow-up. These data are the first observations of PK2 in synovial fluid following traumatic meniscus injury. These findings suggest possible further prognostic indices and therapeutic targets to limit the development of secondary OA. |
| first_indexed | 2024-03-11T01:36:51Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-11T01:36:51Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-1f55468ef4e842d8b6587838dfb8d7622023-11-18T16:52:21ZengMDPI AGJournal of Clinical Medicine2077-03832023-06-011213433010.3390/jcm12134330Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary ResultsMarco Turati0Silvia Franchi1Marco Crippa2Laura Rizzi3Luca Rigamonti4Paola Sacerdote5Simone Daniel Gatti6Massimiliano Piatti7Giulia Galimberti8Daniele Munegato9Giada Amodeo10Robert J. Omeljaniuk11Giovanni Zatti12Antonio Torsello13Marco Bigoni14Orthopedic Department, Fondazione IRCCS San Gerardo dei Tintori, 20900 Monza, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, 20129 Milan, ItalyOrthopedic Department, Fondazione IRCCS San Gerardo dei Tintori, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyTransalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, 20129 Milan, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyTransalpine Center of Pediatric Sports Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, 20129 Milan, ItalyOrthopedic Department, Fondazione IRCCS San Gerardo dei Tintori, 20900 Monza, ItalyDepartment of Pharmacological and Biomolecular Sciences, University of Milan, 20129 Milan, ItalyDepartment of Biology, Lakehead University, Thunder Bay, ON P7B 5E1, CanadaOrthopedic Department, Fondazione IRCCS San Gerardo dei Tintori, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, ItalyKnee osteoarthritis (OA) is a chronic degenerative inflammatory-based condition caused by a cascade of different intra-articular molecules including several cytokines. Among the cytokines, prokineticins (PKs) have recently been identified as important mediators of inflammation and pain. This observational study examined the potential involvement of PK2 in degenerative or traumatic knee disease. Fifteen patients presenting knee osteoarthritis (OA group) and 15 patients presenting a traumatic meniscal tear (TM group) were studied. Synovial fluid samples from affected knees were assessed for PK2, IL-10, and TNF-α using the ELISA method. At a long-term follow-up (minimum 5 years, mean = 6.1 years), patients in the TM group underwent clinical re-evaluation with PROMs (Tegner Activity Scale, IKDC, Lysholm, SKV); in addition, X-ray visualization was used to assess the presence of secondary OA. PK2 was detected in synovial fluids of both TM and OA patients and the levels were comparable between the two groups, while IL-10 levels were significantly greater in the OA group than those in TM patients. PK2 levels correlated with those of IL-10. PK2 levels were greater in blood effusions compared to clear samples, did not differ significantly between sexes, nor were they related to differences in weight, height, or injury (meniscal laterality, time since dosing). No correlation was found between PROMs and radiological classifications in patients in the TM group at final follow-up. These data are the first observations of PK2 in synovial fluid following traumatic meniscus injury. These findings suggest possible further prognostic indices and therapeutic targets to limit the development of secondary OA.https://www.mdpi.com/2077-0383/12/13/4330meniscusosteoarthritiscytokineskneeprokineticinssynovial |
| spellingShingle | Marco Turati Silvia Franchi Marco Crippa Laura Rizzi Luca Rigamonti Paola Sacerdote Simone Daniel Gatti Massimiliano Piatti Giulia Galimberti Daniele Munegato Giada Amodeo Robert J. Omeljaniuk Giovanni Zatti Antonio Torsello Marco Bigoni Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results Journal of Clinical Medicine meniscus osteoarthritis cytokines knee prokineticins synovial |
| title | Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results |
| title_full | Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results |
| title_fullStr | Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results |
| title_full_unstemmed | Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results |
| title_short | Prokineticin 2 and Cytokine Content in the Synovial Fluid of Knee Osteoarthritis and Traumatic Meniscal Tear Patients: Preliminary Results |
| title_sort | prokineticin 2 and cytokine content in the synovial fluid of knee osteoarthritis and traumatic meniscal tear patients preliminary results |
| topic | meniscus osteoarthritis cytokines knee prokineticins synovial |
| url | https://www.mdpi.com/2077-0383/12/13/4330 |
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