Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma
Several air pollution components such as sulfur dioxide, ozone, nitrogen dioxide, and diesel exhaust particulate matter (DEPM) have been linked to the development of asthma. In this study, we investigated the therapeutic potential of three lactic acid bacteria species, <i>Lactobacillus plantar...
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MDPI AG
2020-10-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/11/260 |
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| author | Sun Woo Jin Gi Ho Lee Min Jung Jang Gyeong Eun Hong Jae Young Kim Gi Deok Park Hui Jin Hyun Su Kim Chul Yung Choi Jae Ho Choi Su Gwon Lee Hye Gwang Jeong Yong Pil Hwang |
| author_facet | Sun Woo Jin Gi Ho Lee Min Jung Jang Gyeong Eun Hong Jae Young Kim Gi Deok Park Hui Jin Hyun Su Kim Chul Yung Choi Jae Ho Choi Su Gwon Lee Hye Gwang Jeong Yong Pil Hwang |
| author_sort | Sun Woo Jin |
| collection | DOAJ |
| description | Several air pollution components such as sulfur dioxide, ozone, nitrogen dioxide, and diesel exhaust particulate matter (DEPM) have been linked to the development of asthma. In this study, we investigated the therapeutic potential of three lactic acid bacteria species, <i>Lactobacillus plantarum</i> GREEN CROSS Wellbeing (GCWB)1001, <i>Pediococcus acidilactici</i> GCWB1085, and <i>Lactobacillus rhamnosus</i> GCWB1156, in preventing DEPM-exacerbated asthma in mice. BALB/c mice were first sensitized with ovalbumin (OVA) and were either challenged with OVA or DEPM (DEPM-exacerbated asthma model) by intranasal instillation. All three strains showed no hemolytic activity, suggesting a good safety profile. Oral administration of lactic acid bacteria reduced OVA + DEPM-induced inflammatory infiltration, goblet cell hyperplasia, airway remodeling, and the levels of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF). The probiotics also attenuated OVA + DEPM-induced immunoglobulin E (IgE) levels in serum and in BALF, and significantly reduced caspase-3 activity, total collagen level, and matrix metalloproteinase (MMP)-9 activity. In conclusion, lactic acid bacteria such as <i>L. plantarum</i> GCWB1001, <i>P. acidilactici</i> GCWB1085, and <i>L. rhamnosus</i> treatment in mice with asthma showed significant efficacy in preventing lung inflammation exacerbated by DEPM administration. |
| first_indexed | 2024-03-10T15:16:31Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T15:16:31Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-1f55a90646d64430aded6266791561462023-11-20T18:53:03ZengMDPI AGLife2075-17292020-10-01101126010.3390/life10110260Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of AsthmaSun Woo Jin0Gi Ho Lee1Min Jung Jang2Gyeong Eun Hong3Jae Young Kim4Gi Deok Park5Hui Jin6Hyun Su Kim7Chul Yung Choi8Jae Ho Choi9Su Gwon Lee10Hye Gwang Jeong11Yong Pil Hwang12Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 34134, KoreaDepartment of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 34134, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaDepartment of Research, GREEN CROSS Wellbeing Co., Ltd., Seongnam 13595, KoreaJeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research, Naju 59338, Jeollanamdo, KoreaSubtropical/tropical Organism Gene Bank, Jeju National University, Jeju 63243, KoreaDepartment of Pharmacokinetics, College of Pharmacy, Gachon University, Incheon 21936, KoreaDepartment of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 34134, KoreaDepartment of Pharmaceutical Engineering, International University of Korea, Jinju 52833, KoreaSeveral air pollution components such as sulfur dioxide, ozone, nitrogen dioxide, and diesel exhaust particulate matter (DEPM) have been linked to the development of asthma. In this study, we investigated the therapeutic potential of three lactic acid bacteria species, <i>Lactobacillus plantarum</i> GREEN CROSS Wellbeing (GCWB)1001, <i>Pediococcus acidilactici</i> GCWB1085, and <i>Lactobacillus rhamnosus</i> GCWB1156, in preventing DEPM-exacerbated asthma in mice. BALB/c mice were first sensitized with ovalbumin (OVA) and were either challenged with OVA or DEPM (DEPM-exacerbated asthma model) by intranasal instillation. All three strains showed no hemolytic activity, suggesting a good safety profile. Oral administration of lactic acid bacteria reduced OVA + DEPM-induced inflammatory infiltration, goblet cell hyperplasia, airway remodeling, and the levels of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF). The probiotics also attenuated OVA + DEPM-induced immunoglobulin E (IgE) levels in serum and in BALF, and significantly reduced caspase-3 activity, total collagen level, and matrix metalloproteinase (MMP)-9 activity. In conclusion, lactic acid bacteria such as <i>L. plantarum</i> GCWB1001, <i>P. acidilactici</i> GCWB1085, and <i>L. rhamnosus</i> treatment in mice with asthma showed significant efficacy in preventing lung inflammation exacerbated by DEPM administration.https://www.mdpi.com/2075-1729/10/11/260lactic acid bacteriaDEPMallergic airway inflammationanti-inflammation |
| spellingShingle | Sun Woo Jin Gi Ho Lee Min Jung Jang Gyeong Eun Hong Jae Young Kim Gi Deok Park Hui Jin Hyun Su Kim Chul Yung Choi Jae Ho Choi Su Gwon Lee Hye Gwang Jeong Yong Pil Hwang Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma Life lactic acid bacteria DEPM allergic airway inflammation anti-inflammation |
| title | Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma |
| title_full | Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma |
| title_fullStr | Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma |
| title_full_unstemmed | Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma |
| title_short | Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma |
| title_sort | lactic acid bacteria ameliorate diesel exhaust particulate matter exacerbated allergic inflammation in a murine model of asthma |
| topic | lactic acid bacteria DEPM allergic airway inflammation anti-inflammation |
| url | https://www.mdpi.com/2075-1729/10/11/260 |
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