Biological and Clinicopathological Characteristics of OPN in Cervical Cancers
Background: Cervical cancer (CC) is the most common gynecological malignancy. Recently, an increasing number of studies have indicated that osteopontin (OPN) is a promising diagnostic and prognostic biomarker for CC. However, the biological role and detailed mechanism of OPN in CC remain unclear.Met...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.836509/full |
| _version_ | 1811251020834013184 |
|---|---|
| author | Shuhang Qin Li Yi Yanchun Liang Yili Chen Wei Wang Yuandong Liao Chunyu Zhang Hua Huang Jiaming Huang Shuzhong Yao |
| author_facet | Shuhang Qin Li Yi Yanchun Liang Yili Chen Wei Wang Yuandong Liao Chunyu Zhang Hua Huang Jiaming Huang Shuzhong Yao |
| author_sort | Shuhang Qin |
| collection | DOAJ |
| description | Background: Cervical cancer (CC) is the most common gynecological malignancy. Recently, an increasing number of studies have indicated that osteopontin (OPN) is a promising diagnostic and prognostic biomarker for CC. However, the biological role and detailed mechanism of OPN in CC remain unclear.Methods: The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets and a clinical sample microarray were used in our study. To identify the clinicopathological characteristics of OPN in CC, we compared the expression of OPN between normal and CC tissue samples and analyzed the correlations between OPN expression and multiple clinicopathological features. To identify biological processes involving OPN, OPN-associated genes were screened with Pearson correlation analysis and applied in hallmark gene set enrichment analysis (GSEA). Additionally, leukocyte infiltration was evaluated based on OPN expression. Finally, OPN-related signaling pathways were identified by GSEA.Results: OPN expression was higher in CC samples than in normal tissue samples and positively correlated with age, FIGO stage, tumor size, lymphovascular invasion and an unfavorable prognosis. OPN-associated genes were mainly enriched in the immune response, and increased OPN expression was accompanied by increased M2 macrophage infiltration. Additionally, OPN was correlated with hypoxia, high glycolytic metabolism, apoptosis, angiogenesis, epithelial-mesenchymal transition and multiple signaling pathways (the p53 pathway, the PI3K/Akt pathway, IL6/STAT3 signaling, mTORC1 signaling and KRAS signaling).Conclusion: Our study showed that OPN is involved in immunological activities and multiple tumor processes, identifying it as a potential therapeutic target and useful prognostic factor in CC patients. |
| first_indexed | 2024-04-12T16:13:26Z |
| format | Article |
| id | doaj.art-1f5664aaf9df490f8d1720a9c531d12b |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-12T16:13:26Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-1f5664aaf9df490f8d1720a9c531d12b2022-12-22T03:25:49ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-05-011310.3389/fgene.2022.836509836509Biological and Clinicopathological Characteristics of OPN in Cervical CancersShuhang Qin0Li Yi1Yanchun Liang2Yili Chen3Wei Wang4Yuandong Liao5Chunyu Zhang6Hua Huang7Jiaming Huang8Shuzhong Yao9Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaBackground: Cervical cancer (CC) is the most common gynecological malignancy. Recently, an increasing number of studies have indicated that osteopontin (OPN) is a promising diagnostic and prognostic biomarker for CC. However, the biological role and detailed mechanism of OPN in CC remain unclear.Methods: The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets and a clinical sample microarray were used in our study. To identify the clinicopathological characteristics of OPN in CC, we compared the expression of OPN between normal and CC tissue samples and analyzed the correlations between OPN expression and multiple clinicopathological features. To identify biological processes involving OPN, OPN-associated genes were screened with Pearson correlation analysis and applied in hallmark gene set enrichment analysis (GSEA). Additionally, leukocyte infiltration was evaluated based on OPN expression. Finally, OPN-related signaling pathways were identified by GSEA.Results: OPN expression was higher in CC samples than in normal tissue samples and positively correlated with age, FIGO stage, tumor size, lymphovascular invasion and an unfavorable prognosis. OPN-associated genes were mainly enriched in the immune response, and increased OPN expression was accompanied by increased M2 macrophage infiltration. Additionally, OPN was correlated with hypoxia, high glycolytic metabolism, apoptosis, angiogenesis, epithelial-mesenchymal transition and multiple signaling pathways (the p53 pathway, the PI3K/Akt pathway, IL6/STAT3 signaling, mTORC1 signaling and KRAS signaling).Conclusion: Our study showed that OPN is involved in immunological activities and multiple tumor processes, identifying it as a potential therapeutic target and useful prognostic factor in CC patients.https://www.frontiersin.org/articles/10.3389/fgene.2022.836509/fullosteopontincervical cancerimmunosuppressionbiological processbiomarker |
| spellingShingle | Shuhang Qin Li Yi Yanchun Liang Yili Chen Wei Wang Yuandong Liao Chunyu Zhang Hua Huang Jiaming Huang Shuzhong Yao Biological and Clinicopathological Characteristics of OPN in Cervical Cancers Frontiers in Genetics osteopontin cervical cancer immunosuppression biological process biomarker |
| title | Biological and Clinicopathological Characteristics of OPN in Cervical Cancers |
| title_full | Biological and Clinicopathological Characteristics of OPN in Cervical Cancers |
| title_fullStr | Biological and Clinicopathological Characteristics of OPN in Cervical Cancers |
| title_full_unstemmed | Biological and Clinicopathological Characteristics of OPN in Cervical Cancers |
| title_short | Biological and Clinicopathological Characteristics of OPN in Cervical Cancers |
| title_sort | biological and clinicopathological characteristics of opn in cervical cancers |
| topic | osteopontin cervical cancer immunosuppression biological process biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.836509/full |
| work_keys_str_mv | AT shuhangqin biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT liyi biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT yanchunliang biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT yilichen biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT weiwang biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT yuandongliao biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT chunyuzhang biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT huahuang biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT jiaminghuang biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers AT shuzhongyao biologicalandclinicopathologicalcharacteristicsofopnincervicalcancers |