Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions
The replacement of fishmeal by plant proteins in aquafeeds imposes the use of synthetic methionine (MET) sources to balance the amino acid composition of alternative diets and so to meet the metabolic needs of fish of agronomic interest such as rainbow trout (RT-<i>Oncorhynchus mykiss</i>...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/6/2935 |
| _version_ | 1797470982307315712 |
|---|---|
| author | Karine Pinel Cécile Heraud Guillaume Morin Karine Dias Annaëlle Marcé Linda Beauclair Stéphanie Fontagné-Dicharry Karthik Masagounder Martina Klünemann Iban Seiliez Florian Beaumatin |
| author_facet | Karine Pinel Cécile Heraud Guillaume Morin Karine Dias Annaëlle Marcé Linda Beauclair Stéphanie Fontagné-Dicharry Karthik Masagounder Martina Klünemann Iban Seiliez Florian Beaumatin |
| author_sort | Karine Pinel |
| collection | DOAJ |
| description | The replacement of fishmeal by plant proteins in aquafeeds imposes the use of synthetic methionine (MET) sources to balance the amino acid composition of alternative diets and so to meet the metabolic needs of fish of agronomic interest such as rainbow trout (RT-<i>Oncorhynchus mykiss</i>). Nonetheless, debates still exist to determine if one MET source is more efficiently used than another by fish. To address this question, the use of fish cell lines appeared a convenient strategy, since it allowed to perfectly control cell growing conditions notably by fully depleting MET from the media and studying which MET source is capable to restore cell growth/proliferation and metabolism when supplemented back. Thus, results of cell proliferation assays, Western blots, RT-qPCR and liquid chromatography analyses from two RT liver-derived cell lines revealed a better absorption and metabolization of DL-MET than DL-Methionine Hydroxy Analog (MHA) with the activation of the mechanistic Target Of Rapamycin (mTOR) pathway for DL-MET and the activation of integrated stress response (ISR) pathway for MHA. Altogether, the results clearly allow to conclude that both synthetic MET sources are not biologically equivalent, suggesting similar in vivo effects in RT liver and, therefore, questioning the MHA efficiencies in other RT tissues. |
| first_indexed | 2024-03-09T19:44:04Z |
| format | Article |
| id | doaj.art-1f56a0c7b89a4734b6e0f8b439827fc7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:44:04Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1f56a0c7b89a4734b6e0f8b439827fc72023-11-24T01:29:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236293510.3390/ijms23062935Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines FunctionsKarine Pinel0Cécile Heraud1Guillaume Morin2Karine Dias3Annaëlle Marcé4Linda Beauclair5Stéphanie Fontagné-Dicharry6Karthik Masagounder7Martina Klünemann8Iban Seiliez9Florian Beaumatin10Université de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceEvonik Operations GmbH, Rodenbacher Chaussee 4, 4D-63457 Hanau, GermanyEvonik Operations GmbH, Rodenbacher Chaussee 4, 4D-63457 Hanau, GermanyUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceThe replacement of fishmeal by plant proteins in aquafeeds imposes the use of synthetic methionine (MET) sources to balance the amino acid composition of alternative diets and so to meet the metabolic needs of fish of agronomic interest such as rainbow trout (RT-<i>Oncorhynchus mykiss</i>). Nonetheless, debates still exist to determine if one MET source is more efficiently used than another by fish. To address this question, the use of fish cell lines appeared a convenient strategy, since it allowed to perfectly control cell growing conditions notably by fully depleting MET from the media and studying which MET source is capable to restore cell growth/proliferation and metabolism when supplemented back. Thus, results of cell proliferation assays, Western blots, RT-qPCR and liquid chromatography analyses from two RT liver-derived cell lines revealed a better absorption and metabolization of DL-MET than DL-Methionine Hydroxy Analog (MHA) with the activation of the mechanistic Target Of Rapamycin (mTOR) pathway for DL-MET and the activation of integrated stress response (ISR) pathway for MHA. Altogether, the results clearly allow to conclude that both synthetic MET sources are not biologically equivalent, suggesting similar in vivo effects in RT liver and, therefore, questioning the MHA efficiencies in other RT tissues.https://www.mdpi.com/1422-0067/23/6/2935aquaculturenutritionrainbow troutcell linesmetabolismmethionine |
| spellingShingle | Karine Pinel Cécile Heraud Guillaume Morin Karine Dias Annaëlle Marcé Linda Beauclair Stéphanie Fontagné-Dicharry Karthik Masagounder Martina Klünemann Iban Seiliez Florian Beaumatin Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions International Journal of Molecular Sciences aquaculture nutrition rainbow trout cell lines metabolism methionine |
| title | Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions |
| title_full | Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions |
| title_fullStr | Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions |
| title_full_unstemmed | Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions |
| title_short | Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions |
| title_sort | are the main methionine sources equivalent a focus on dl methionine and dl methionine hydroxy analog reveals differences on rainbow trout hepatic cell lines functions |
| topic | aquaculture nutrition rainbow trout cell lines metabolism methionine |
| url | https://www.mdpi.com/1422-0067/23/6/2935 |
| work_keys_str_mv | AT karinepinel arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT cecileheraud arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT guillaumemorin arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT karinedias arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT annaellemarce arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT lindabeauclair arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT stephaniefontagnedicharry arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT karthikmasagounder arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT martinaklunemann arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT ibanseiliez arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions AT florianbeaumatin arethemainmethioninesourcesequivalentafocusondlmethionineanddlmethioninehydroxyanalogrevealsdifferencesonrainbowtrouthepaticcelllinesfunctions |