Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery
<i>Toxoplasma gondii</i>, a protozoan parasite with the ability to infect various warm-blooded vertebrates, including humans, is the causative agent of toxoplasmosis. This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/1/33 |
| _version_ | 1797342775594713088 |
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| author | Bin Deng Laura Vanagas Andres M. Alonso Sergio O. Angel |
| author_facet | Bin Deng Laura Vanagas Andres M. Alonso Sergio O. Angel |
| author_sort | Bin Deng |
| collection | DOAJ |
| description | <i>Toxoplasma gondii</i>, a protozoan parasite with the ability to infect various warm-blooded vertebrates, including humans, is the causative agent of toxoplasmosis. This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially affecting the fetus through congenital transmission. A comprehensive understanding of the intricate molecular interactions between <i>T. gondii</i> and its host is pivotal for the development of effective therapeutic strategies. This review emphasizes the crucial role of proteomics in <i>T. gondii</i> research, with a specific focus on host–parasite interactions, post-translational modifications (PTMs), PTM crosstalk, and ongoing efforts in drug discovery. Additionally, we provide an overview of recent advancements in proteomics techniques, encompassing interactome sample preparation methods such as BioID (BirA*-mediated proximity-dependent biotin identification), APEX (ascorbate peroxidase-mediated proximity labeling), and Y2H (yeast two hybrid), as well as various proteomics approaches, including single-cell analysis, DIA (data-independent acquisition), targeted, top-down, and plasma proteomics. Furthermore, we discuss bioinformatics and the integration of proteomics with other omics technologies, highlighting its potential in unraveling the intricate mechanisms of <i>T. gondii</i> pathogenesis and identifying novel therapeutic targets. |
| first_indexed | 2024-03-08T10:38:09Z |
| format | Article |
| id | doaj.art-1f56a1df9d3249dab9b4d3201b099847 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-08T10:38:09Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-1f56a1df9d3249dab9b4d3201b0998472024-01-26T18:03:02ZengMDPI AGPathogens2076-08172023-12-011313310.3390/pathogens13010033Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target DiscoveryBin Deng0Laura Vanagas1Andres M. Alonso2Sergio O. Angel3Department of Biology and VBRN Proteomics Facility, University of Vermont, Burlington, VT 05405, USALaboratorio de Parasitología Molecular, Instituto Tecnológico de Chascomús (CONICET-UNSAM), Chascomús 7130, Provincia de Buenos Aires, ArgentinaLaboratorio de Parasitología Molecular, Instituto Tecnológico de Chascomús (CONICET-UNSAM), Chascomús 7130, Provincia de Buenos Aires, ArgentinaLaboratorio de Parasitología Molecular, Instituto Tecnológico de Chascomús (CONICET-UNSAM), Chascomús 7130, Provincia de Buenos Aires, Argentina<i>Toxoplasma gondii</i>, a protozoan parasite with the ability to infect various warm-blooded vertebrates, including humans, is the causative agent of toxoplasmosis. This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially affecting the fetus through congenital transmission. A comprehensive understanding of the intricate molecular interactions between <i>T. gondii</i> and its host is pivotal for the development of effective therapeutic strategies. This review emphasizes the crucial role of proteomics in <i>T. gondii</i> research, with a specific focus on host–parasite interactions, post-translational modifications (PTMs), PTM crosstalk, and ongoing efforts in drug discovery. Additionally, we provide an overview of recent advancements in proteomics techniques, encompassing interactome sample preparation methods such as BioID (BirA*-mediated proximity-dependent biotin identification), APEX (ascorbate peroxidase-mediated proximity labeling), and Y2H (yeast two hybrid), as well as various proteomics approaches, including single-cell analysis, DIA (data-independent acquisition), targeted, top-down, and plasma proteomics. Furthermore, we discuss bioinformatics and the integration of proteomics with other omics technologies, highlighting its potential in unraveling the intricate mechanisms of <i>T. gondii</i> pathogenesis and identifying novel therapeutic targets.https://www.mdpi.com/2076-0817/13/1/33<i>Toxoplasma gondii</i>proteomicshost–parasite interactionsPTMsdrug discovery |
| spellingShingle | Bin Deng Laura Vanagas Andres M. Alonso Sergio O. Angel Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery Pathogens <i>Toxoplasma gondii</i> proteomics host–parasite interactions PTMs drug discovery |
| title | Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery |
| title_full | Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery |
| title_fullStr | Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery |
| title_full_unstemmed | Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery |
| title_short | Proteomics Applications in <i>Toxoplasma gondii</i>: Unveiling the Host–Parasite Interactions and Therapeutic Target Discovery |
| title_sort | proteomics applications in i toxoplasma gondii i unveiling the host parasite interactions and therapeutic target discovery |
| topic | <i>Toxoplasma gondii</i> proteomics host–parasite interactions PTMs drug discovery |
| url | https://www.mdpi.com/2076-0817/13/1/33 |
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