Diversity of Late Blight Resistance Genes in the VIR Potato Collection
Late blight (LB) caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to <i>P. infestans</i> (<i...
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MDPI AG
2023-01-01
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| author | Elena V. Rogozina Alyona A. Gurina Nadezhda A. Chalaya Nadezhda M. Zoteyeva Mariya A. Kuznetsova Mariya P. Beketova Oksana A. Muratova Ekaterina A. Sokolova Polina E. Drobyazina Emil E. Khavkin |
| author_facet | Elena V. Rogozina Alyona A. Gurina Nadezhda A. Chalaya Nadezhda M. Zoteyeva Mariya A. Kuznetsova Mariya P. Beketova Oksana A. Muratova Ekaterina A. Sokolova Polina E. Drobyazina Emil E. Khavkin |
| author_sort | Elena V. Rogozina |
| collection | DOAJ |
| description | Late blight (LB) caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to <i>P. infestans</i> (<i>Rpi</i> genes). Such genes have been discovered in highly diverse wild, primitive, and cultivated species of tuber-bearing potatoes (<i>Solanum</i> L. section <i>Petota</i> Dumort.) and introgressed into the elite potato cultivars by hybridization and transgenic complementation. Unfortunately, even the most resistant potato varieties have been overcome by LB due to the arrival of new pathogen strains and their rapid evolution. Therefore, novel sources for germplasm enhancement comprising the broad-spectrum <i>Rpi</i> genes are in high demand with breeders who aim to provide durable LB resistance. The Genbank of the N.I. Vavilov Institute of Plant Genetic Resources (VIR) in St. Petersburg harbors one of the world’s largest collections of potato and potato relatives. In this study, LB resistance was evaluated in a core selection representing 20 species of seven <i>Petota</i> series according to the Hawkes (1990) classification: <i>Bulbocastana</i> (Rydb.) Hawkes, <i>Demissa</i> Buk., <i>Longipedicellata</i> Buk., <i>Maglia</i> Bitt., <i>Pinnatisecta</i> (Rydb.) Hawkes, <i>Tuberosa</i> (Rydb.) Hawkes (wild and cultivated species), and <i>Yungasensa</i> Corr. LB resistance was assessed in 96 accessions representing 18 species in the laboratory test with detached leaves using a highly virulent and aggressive isolate of <i>P. infestans</i>. The <i>Petota</i> species notably differed in their LB resistance: <i>S. bulbocastanum</i> Dun., <i>S. demissum</i> Lindl., <i>S. cardiophyllum</i> Lindl., and <i>S. berthaultii</i> Hawkes stood out at a high frequency of resistant accessions (7–9 points on a 9-point scale). Well-established specific SCAR markers of ten <i>Rpi</i> genes—<i>Rpi-R1</i>, <i>Rpi-R2/Rpi-blb3</i>, <i>Rpi-R3a</i>, <i>Rpi-R3b</i>, <i>Rpi-R8</i>, <i>Rpi-blb1/Rpi-sto1</i>, <i>Rpi-blb2,</i> and <i>Rpi-vnt1</i>—were used to mine 117 accessions representing 20 species from seven <i>Petota</i> series. In particular, our evidence confirmed the diverse <i>Rpi</i> gene location in two American continents. The structural homologs of the <i>Rpi-R2</i>, <i>Rpi-R3a</i>, <i>Rpi-R3b,</i> and <i>Rpi-R8</i> genes were found in the North American species other than <i>S. demissum</i>, the species that was the original source of these genes for early potato breeding, and in some cases, in the South American <i>Tuberosa</i> species. The <i>Rpi-blb1/Rpi-sto1</i> orthologs from <i>S. bulbocastanum</i> and <i>S. stoloniferum</i> Schlechtd et Bché were restricted to genome B in the Mesoamerican series <i>Bulbocastana</i>, <i>Pinnatisecta,</i> and <i>Longipedicellata</i>. The structural homologs of the <i>Rpi-vnt1</i> gene that were initially identified in the South American species <i>S. venturii</i> Hawkes and Hjert. were reported, for the first time, in the North American series of <i>Petota</i> species. |
| first_indexed | 2024-03-09T11:25:03Z |
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| language | English |
| last_indexed | 2024-03-09T11:25:03Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-1f57a8db6233426eadb7bfe58f01aab82023-12-01T00:03:49ZengMDPI AGPlants2223-77472023-01-0112227310.3390/plants12020273Diversity of Late Blight Resistance Genes in the VIR Potato CollectionElena V. Rogozina0Alyona A. Gurina1Nadezhda A. Chalaya2Nadezhda M. Zoteyeva3Mariya A. Kuznetsova4Mariya P. Beketova5Oksana A. Muratova6Ekaterina A. Sokolova7Polina E. Drobyazina8Emil E. Khavkin9N.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg 190000, RussiaN.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg 190000, RussiaN.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg 190000, RussiaN.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg 190000, RussiaInstitute of Phytopathology, Bol’shiye Vyazemy, Moscow 143050, RussiaInstitute of Agricultural Biotechnology, Moscow 127550, RussiaInstitute of Agricultural Biotechnology, Moscow 127550, RussiaInstitute of Agricultural Biotechnology, Moscow 127550, RussiaInstitute of Agricultural Biotechnology, Moscow 127550, RussiaInstitute of Agricultural Biotechnology, Moscow 127550, RussiaLate blight (LB) caused by the oomycete <i>Phytophthora infestans</i> (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to <i>P. infestans</i> (<i>Rpi</i> genes). Such genes have been discovered in highly diverse wild, primitive, and cultivated species of tuber-bearing potatoes (<i>Solanum</i> L. section <i>Petota</i> Dumort.) and introgressed into the elite potato cultivars by hybridization and transgenic complementation. Unfortunately, even the most resistant potato varieties have been overcome by LB due to the arrival of new pathogen strains and their rapid evolution. Therefore, novel sources for germplasm enhancement comprising the broad-spectrum <i>Rpi</i> genes are in high demand with breeders who aim to provide durable LB resistance. The Genbank of the N.I. Vavilov Institute of Plant Genetic Resources (VIR) in St. Petersburg harbors one of the world’s largest collections of potato and potato relatives. In this study, LB resistance was evaluated in a core selection representing 20 species of seven <i>Petota</i> series according to the Hawkes (1990) classification: <i>Bulbocastana</i> (Rydb.) Hawkes, <i>Demissa</i> Buk., <i>Longipedicellata</i> Buk., <i>Maglia</i> Bitt., <i>Pinnatisecta</i> (Rydb.) Hawkes, <i>Tuberosa</i> (Rydb.) Hawkes (wild and cultivated species), and <i>Yungasensa</i> Corr. LB resistance was assessed in 96 accessions representing 18 species in the laboratory test with detached leaves using a highly virulent and aggressive isolate of <i>P. infestans</i>. The <i>Petota</i> species notably differed in their LB resistance: <i>S. bulbocastanum</i> Dun., <i>S. demissum</i> Lindl., <i>S. cardiophyllum</i> Lindl., and <i>S. berthaultii</i> Hawkes stood out at a high frequency of resistant accessions (7–9 points on a 9-point scale). Well-established specific SCAR markers of ten <i>Rpi</i> genes—<i>Rpi-R1</i>, <i>Rpi-R2/Rpi-blb3</i>, <i>Rpi-R3a</i>, <i>Rpi-R3b</i>, <i>Rpi-R8</i>, <i>Rpi-blb1/Rpi-sto1</i>, <i>Rpi-blb2,</i> and <i>Rpi-vnt1</i>—were used to mine 117 accessions representing 20 species from seven <i>Petota</i> series. In particular, our evidence confirmed the diverse <i>Rpi</i> gene location in two American continents. The structural homologs of the <i>Rpi-R2</i>, <i>Rpi-R3a</i>, <i>Rpi-R3b,</i> and <i>Rpi-R8</i> genes were found in the North American species other than <i>S. demissum</i>, the species that was the original source of these genes for early potato breeding, and in some cases, in the South American <i>Tuberosa</i> species. The <i>Rpi-blb1/Rpi-sto1</i> orthologs from <i>S. bulbocastanum</i> and <i>S. stoloniferum</i> Schlechtd et Bché were restricted to genome B in the Mesoamerican series <i>Bulbocastana</i>, <i>Pinnatisecta,</i> and <i>Longipedicellata</i>. The structural homologs of the <i>Rpi-vnt1</i> gene that were initially identified in the South American species <i>S. venturii</i> Hawkes and Hjert. were reported, for the first time, in the North American series of <i>Petota</i> species.https://www.mdpi.com/2223-7747/12/2/273<i>Phytophthora infestans</i><i>Solanum</i> section <i>Petota</i>genetic diversitywild potato relativesgermplasm enrichmentlate blight resistance |
| spellingShingle | Elena V. Rogozina Alyona A. Gurina Nadezhda A. Chalaya Nadezhda M. Zoteyeva Mariya A. Kuznetsova Mariya P. Beketova Oksana A. Muratova Ekaterina A. Sokolova Polina E. Drobyazina Emil E. Khavkin Diversity of Late Blight Resistance Genes in the VIR Potato Collection Plants <i>Phytophthora infestans</i> <i>Solanum</i> section <i>Petota</i> genetic diversity wild potato relatives germplasm enrichment late blight resistance |
| title | Diversity of Late Blight Resistance Genes in the VIR Potato Collection |
| title_full | Diversity of Late Blight Resistance Genes in the VIR Potato Collection |
| title_fullStr | Diversity of Late Blight Resistance Genes in the VIR Potato Collection |
| title_full_unstemmed | Diversity of Late Blight Resistance Genes in the VIR Potato Collection |
| title_short | Diversity of Late Blight Resistance Genes in the VIR Potato Collection |
| title_sort | diversity of late blight resistance genes in the vir potato collection |
| topic | <i>Phytophthora infestans</i> <i>Solanum</i> section <i>Petota</i> genetic diversity wild potato relatives germplasm enrichment late blight resistance |
| url | https://www.mdpi.com/2223-7747/12/2/273 |
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