Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1
The Twort-like myoviruses (<i>Kayvirus</i> genus) of <i>S. aureus</i> are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology b...
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MDPI AG
2020-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/11/1320 |
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| author | Maria Kornienko Gleb Fisunov Dmitry Bespiatykh Nikita Kuptsov Roman Gorodnichev Ksenia Klimina Eugene Kulikov Elena Ilina Andrey Letarov Egor Shitikov |
| author_facet | Maria Kornienko Gleb Fisunov Dmitry Bespiatykh Nikita Kuptsov Roman Gorodnichev Ksenia Klimina Eugene Kulikov Elena Ilina Andrey Letarov Egor Shitikov |
| author_sort | Maria Kornienko |
| collection | DOAJ |
| description | The Twort-like myoviruses (<i>Kayvirus</i> genus) of <i>S. aureus</i> are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the <i>Kayvirus</i> genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp). |
| first_indexed | 2024-03-10T14:46:31Z |
| format | Article |
| id | doaj.art-1f582c64c61f45479cd3d5052d99988b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T14:46:31Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1f582c64c61f45479cd3d5052d99988b2023-11-20T21:17:34ZengMDPI AGViruses1999-49152020-11-011211132010.3390/v12111320Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1Maria Kornienko0Gleb Fisunov1Dmitry Bespiatykh2Nikita Kuptsov3Roman Gorodnichev4Ksenia Klimina5Eugene Kulikov6Elena Ilina7Andrey Letarov8Egor Shitikov9Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, Winogradsky Institute of Microbiology, 117312 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences, Winogradsky Institute of Microbiology, 117312 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaThe Twort-like myoviruses (<i>Kayvirus</i> genus) of <i>S. aureus</i> are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the <i>Kayvirus</i> genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp).https://www.mdpi.com/1999-4915/12/11/1320transcriptome analysis<i>Kayvirus</i>bacteriophagepromoterterminatorRNAseq |
| spellingShingle | Maria Kornienko Gleb Fisunov Dmitry Bespiatykh Nikita Kuptsov Roman Gorodnichev Ksenia Klimina Eugene Kulikov Elena Ilina Andrey Letarov Egor Shitikov Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 Viruses transcriptome analysis <i>Kayvirus</i> bacteriophage promoter terminator RNAseq |
| title | Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 |
| title_full | Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 |
| title_fullStr | Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 |
| title_full_unstemmed | Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 |
| title_short | Transcriptional Landscape of <i>Staphylococcus aureus Kayvirus</i> Bacteriophage vB_SauM-515A1 |
| title_sort | transcriptional landscape of i staphylococcus aureus kayvirus i bacteriophage vb saum 515a1 |
| topic | transcriptome analysis <i>Kayvirus</i> bacteriophage promoter terminator RNAseq |
| url | https://www.mdpi.com/1999-4915/12/11/1320 |
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