Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis
Abstract Background Ocular manifestations in dogs with leishmaniasis are frequent and complications in affected tissues can lead to blindness. Immune processes play a very important role in the pathogenesis of ocular inflammation. Therefore, the immunology of ocular manifestations in dogs with leish...
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.982 |
| _version_ | 1797866280135426048 |
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| author | Amel F. El Goulli Lilia Zribi Rania Sanhaji Ahmed Chabchoub Aïda Bouratbine Mohamed Gharbi Hafedh Abdelmelek |
| author_facet | Amel F. El Goulli Lilia Zribi Rania Sanhaji Ahmed Chabchoub Aïda Bouratbine Mohamed Gharbi Hafedh Abdelmelek |
| author_sort | Amel F. El Goulli |
| collection | DOAJ |
| description | Abstract Background Ocular manifestations in dogs with leishmaniasis are frequent and complications in affected tissues can lead to blindness. Immune processes play a very important role in the pathogenesis of ocular inflammation. Therefore, the immunology of ocular manifestations in dogs with leishmaniasis remains complex and poorly understood. Objectives Estimation and characterisation of ocular and periocular manifestations in dogs naturally infected with Leishmania infantum and investigation of the production site of specific anti‐Leishmania infantum IgG. Methods The present investigation used 53 confirmed dogs infected with Leishmania infantum, presenting ocular and periocular lesions, and 10 control non‐infected dogs. Complete macroscopic ophthalmic examination of eyelids and globes was performed. Both total and anti‐Leishmania infantum IgG antibodies were studied in sera and aqueous humour (AH) of all dogs by ELISA technique. A Goldmann–Witmer coefficient (C value) was calculated. Results The main ophthalmological findings were keratoconjunctivitis (71.7%; 38/53), hyperplasia of conjunctival lymphoid follicles (54.7%; 29/53), blepharitis (50.9%; 27/53) and uveitis (20.7%; 11/53). Ocular production of anti‐Leishmania infantum IgG was detected in 73.6% (39/53) of infected dogs. There was no correlation between the antibody levels in AH and sera of the same dog. The mean anti‐Leishmania infantum IgG in AH was higher in uveitis, followed by lesions affecting only the adnexa (p < 0.0001). The highest mean C values were observed for uveitis, conjunctivitis and keratitis. Conclusions Our findings suggest that production of anti‐Leishmania IgG in dogs infected with Leishmania infantum with ocular manifestations begin in situ and follows by a transfer of antibodies from the bloodstream to the AH. |
| first_indexed | 2024-04-09T23:21:29Z |
| format | Article |
| id | doaj.art-1f5ac57c474e440eaca22974f0a9f105 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-09T23:21:29Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-1f5ac57c474e440eaca22974f0a9f1052023-03-21T15:53:53ZengWileyVeterinary Medicine and Science2053-10952023-03-019262563710.1002/vms3.982Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasisAmel F. El Goulli0Lilia Zribi1Rania Sanhaji2Ahmed Chabchoub3Aïda Bouratbine4Mohamed Gharbi5Hafedh Abdelmelek6Internal Equine and Small Animal Medicine Service, Clinical Department, National School of Veterinary Medicine Institution of Agricultural Research and Higher Education University of Manouba Sidi Thabet TunisiaDepartment of Clinical Parasitology, Research Laboratory LR 11‐IPT‐06 ‘Medical Parasitology Biotechnology and Biomolecules’ Pasteur Institute of Tunis University of Tunis El Manar Tunis TunisiaInternal Equine and Small Animal Medicine Service, Clinical Department, National School of Veterinary Medicine Institution of Agricultural Research and Higher Education University of Manouba Sidi Thabet TunisiaInternal Equine and Small Animal Medicine Service, Clinical Department, National School of Veterinary Medicine Institution of Agricultural Research and Higher Education University of Manouba Sidi Thabet TunisiaDepartment of Clinical Parasitology, Research Laboratory LR 11‐IPT‐06 ‘Medical Parasitology Biotechnology and Biomolecules’ Pasteur Institute of Tunis University of Tunis El Manar Tunis TunisiaLaboratory of Parasitology, National School of Veterinary Medicine, Institution of Agricultural Research and Higher Education University of Manouba Sidi Thabet TunisiaLaboratory of Integrated Physiology Faculty of Sciences of Bizerte University of Carthage Jarzouna TunisiaAbstract Background Ocular manifestations in dogs with leishmaniasis are frequent and complications in affected tissues can lead to blindness. Immune processes play a very important role in the pathogenesis of ocular inflammation. Therefore, the immunology of ocular manifestations in dogs with leishmaniasis remains complex and poorly understood. Objectives Estimation and characterisation of ocular and periocular manifestations in dogs naturally infected with Leishmania infantum and investigation of the production site of specific anti‐Leishmania infantum IgG. Methods The present investigation used 53 confirmed dogs infected with Leishmania infantum, presenting ocular and periocular lesions, and 10 control non‐infected dogs. Complete macroscopic ophthalmic examination of eyelids and globes was performed. Both total and anti‐Leishmania infantum IgG antibodies were studied in sera and aqueous humour (AH) of all dogs by ELISA technique. A Goldmann–Witmer coefficient (C value) was calculated. Results The main ophthalmological findings were keratoconjunctivitis (71.7%; 38/53), hyperplasia of conjunctival lymphoid follicles (54.7%; 29/53), blepharitis (50.9%; 27/53) and uveitis (20.7%; 11/53). Ocular production of anti‐Leishmania infantum IgG was detected in 73.6% (39/53) of infected dogs. There was no correlation between the antibody levels in AH and sera of the same dog. The mean anti‐Leishmania infantum IgG in AH was higher in uveitis, followed by lesions affecting only the adnexa (p < 0.0001). The highest mean C values were observed for uveitis, conjunctivitis and keratitis. Conclusions Our findings suggest that production of anti‐Leishmania IgG in dogs infected with Leishmania infantum with ocular manifestations begin in situ and follows by a transfer of antibodies from the bloodstream to the AH.https://doi.org/10.1002/vms3.982antibodyaqueous humourdogeyeGoldmann–Witmer coefficientleishmaniasis |
| spellingShingle | Amel F. El Goulli Lilia Zribi Rania Sanhaji Ahmed Chabchoub Aïda Bouratbine Mohamed Gharbi Hafedh Abdelmelek Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis Veterinary Medicine and Science antibody aqueous humour dog eye Goldmann–Witmer coefficient leishmaniasis |
| title | Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| title_full | Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| title_fullStr | Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| title_full_unstemmed | Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| title_short | Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| title_sort | study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis |
| topic | antibody aqueous humour dog eye Goldmann–Witmer coefficient leishmaniasis |
| url | https://doi.org/10.1002/vms3.982 |
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