Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção
In order to investigate the IgG HIV-1 antibodies rectivity to structural components of the virus, 85 sera from infected Brazilians, comprising the total spectrum of HIV infection, were analysed by Western blot assay. The sera were confirmed as being positive to HIV with enzyme linked immuno assay (E...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1989-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000100003 |
| _version_ | 1797713471059525632 |
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| author | Jairo Ivo dos Santos Deise Luci Campos Bernardo Galvão Castro |
| author_facet | Jairo Ivo dos Santos Deise Luci Campos Bernardo Galvão Castro |
| author_sort | Jairo Ivo dos Santos |
| collection | DOAJ |
| description | In order to investigate the IgG HIV-1 antibodies rectivity to structural components of the virus, 85 sera from infected Brazilians, comprising the total spectrum of HIV infection, were analysed by Western blot assay. The sera were confirmed as being positive to HIV with enzyme linked immuno assay (ELISA) and indirect immunofluorescence (IIF). Although the sera from patients reacted less intensively to the gag polypeptide of 55KDa, no distinctive antigen reaction patterns were observed between sera patients with different clinical forms. Because of the higher frequency of reactivity to the gag p24 in AIDS patients, the patterns of anti-HIV IgG responses are similar to those observed in their African counterparts.<br>Com o objetivo de avaliar a resposta de anticorpos da classe IgG a componentes estruturais do vírus, 85 soros de pacientes brasileiros, compreendendo todo o espectro da infecção pelo HIV foram analisados pela técnica de Western blot. Os soros confirmados como positivos pelas técnicas de imunofluorescência indireta e imunoenzimática. Embora os soros de alguns pacientes estudados reagissem menos intensamente com o polipeptídeo de 55 KDa, não observamos nenhuma diferença de reatividade entre os soros de pacientes com as diversas forma clínicas. Entretanto, a grande freqüência de reatividade ao polipeptídeo de 24 KDa nos pacientes com AIDS sugere que o padrão de resposta imune seja similar aos pacientes Africanos. |
| first_indexed | 2024-03-12T07:37:06Z |
| format | Article |
| id | doaj.art-1f5d0fdcbb2f44438f15a5c1fee14ef6 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:37:06Z |
| publishDate | 1989-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1f5d0fdcbb2f44438f15a5c1fee14ef62023-09-02T21:28:41ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601989-03-0184191110.1590/S0074-02761989000100003Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecçãoJairo Ivo dos SantosDeise Luci CamposBernardo Galvão CastroIn order to investigate the IgG HIV-1 antibodies rectivity to structural components of the virus, 85 sera from infected Brazilians, comprising the total spectrum of HIV infection, were analysed by Western blot assay. The sera were confirmed as being positive to HIV with enzyme linked immuno assay (ELISA) and indirect immunofluorescence (IIF). Although the sera from patients reacted less intensively to the gag polypeptide of 55KDa, no distinctive antigen reaction patterns were observed between sera patients with different clinical forms. Because of the higher frequency of reactivity to the gag p24 in AIDS patients, the patterns of anti-HIV IgG responses are similar to those observed in their African counterparts.<br>Com o objetivo de avaliar a resposta de anticorpos da classe IgG a componentes estruturais do vírus, 85 soros de pacientes brasileiros, compreendendo todo o espectro da infecção pelo HIV foram analisados pela técnica de Western blot. Os soros confirmados como positivos pelas técnicas de imunofluorescência indireta e imunoenzimática. Embora os soros de alguns pacientes estudados reagissem menos intensamente com o polipeptídeo de 55 KDa, não observamos nenhuma diferença de reatividade entre os soros de pacientes com as diversas forma clínicas. Entretanto, a grande freqüência de reatividade ao polipeptídeo de 24 KDa nos pacientes com AIDS sugere que o padrão de resposta imune seja similar aos pacientes Africanos.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000100003AIDSHIVsorologiaWestern blotAIDSHIVserologyWestern blot |
| spellingShingle | Jairo Ivo dos Santos Deise Luci Campos Bernardo Galvão Castro Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção Memorias do Instituto Oswaldo Cruz AIDS HIV sorologia Western blot AIDS HIV serology Western blot |
| title | Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção |
| title_full | Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção |
| title_fullStr | Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção |
| title_full_unstemmed | Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção |
| title_short | Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção |
| title_sort | patterns of serologic response to human immunodeficiency virus type 1 hiv 1 in brazilians with different clinical forms of hiv infection padroes de resposta sorologica ao virus da imunodeficiencia humana do tipo 1 hiv 1 em brasilieros com diferentes formas clinicas de infeccao |
| topic | AIDS HIV sorologia Western blot AIDS HIV serology Western blot |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000100003 |
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