The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey
In this study the effects of different sucrose concentrations, and the combinations of jasmonic acid (JA) with auxins (IAA or NAA) or with cytokinin (2iP) on the bulb induction and rooting of in vitro plantlets of Hyacinthus orientalis subsp. chionophyllus Wendelbo, which is endemic in Turkey, were...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2020-08-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3473 |
| _version_ | 1797922865184505856 |
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| author | Selay Doğan Gülat Çağlar Esra Bulunuz Palaz |
| author_facet | Selay Doğan Gülat Çağlar Esra Bulunuz Palaz |
| author_sort | Selay Doğan |
| collection | DOAJ |
| description | In this study the effects of different sucrose concentrations, and the combinations of jasmonic acid (JA) with auxins (IAA or NAA) or with cytokinin (2iP) on the bulb induction and rooting of in vitro plantlets of Hyacinthus orientalis subsp. chionophyllus Wendelbo, which is endemic in Turkey, were investigated. The effect of four different sucrose concentrations (30, 45, 60 and 90 g L-1) on bulb formation in tissue culture was investigated. These plantlets were cultured on MS medium supplemented with several concentrations and combinations of JA (0.0, 1.0, 2.0 mg L-1) and 2iP (0.0, 0.25 and 0.50 mg L-1), IAA or NAA (0.5, 1.0 mg L-1). In JA- 2iP treatment, the highest number of bulblets (13.7 number/explant) was obtained by the combinations of JA 1.0 mg L-1 + 2iP 0.25 mg L-1. Also, the largest bulblets with the mean diameter of 7.9 mm were found on MS medium supplemented with JA 2.0 mg L-1. In JA – Auxin treatment, the mean root number per bulblet was highest (17.9 number/explant) and root formation rate was maximum (81.14%) on MS medium supplemented with IAA 1.0 mg L-1 + JA 2.0 mg L-1. |
| first_indexed | 2024-04-10T14:39:02Z |
| format | Article |
| id | doaj.art-1f62a11272bb4692ba4f89095f637378 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T14:39:02Z |
| publishDate | 2020-08-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-1f62a11272bb4692ba4f89095f6373782023-02-15T16:08:19ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2020-08-01881713171910.24925/turjaf.v8i8.1713-1719.34731728The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in TurkeySelay Doğan0Gülat Çağlar1Esra Bulunuz Palaz2Biological Diversity and Genetic Resources Department, Aegean Agricultural Research Institute, 35661 İzmirDepartment of Horticulture, Faculty of Agriculture, Kahramanmaraş Sütçü Imam University, 46050 Kahramanmaraş, TurkeyEast Mediterranean Transitional Zone Agricultural Research Institute, 46050 Kahramanmaraş,In this study the effects of different sucrose concentrations, and the combinations of jasmonic acid (JA) with auxins (IAA or NAA) or with cytokinin (2iP) on the bulb induction and rooting of in vitro plantlets of Hyacinthus orientalis subsp. chionophyllus Wendelbo, which is endemic in Turkey, were investigated. The effect of four different sucrose concentrations (30, 45, 60 and 90 g L-1) on bulb formation in tissue culture was investigated. These plantlets were cultured on MS medium supplemented with several concentrations and combinations of JA (0.0, 1.0, 2.0 mg L-1) and 2iP (0.0, 0.25 and 0.50 mg L-1), IAA or NAA (0.5, 1.0 mg L-1). In JA- 2iP treatment, the highest number of bulblets (13.7 number/explant) was obtained by the combinations of JA 1.0 mg L-1 + 2iP 0.25 mg L-1. Also, the largest bulblets with the mean diameter of 7.9 mm were found on MS medium supplemented with JA 2.0 mg L-1. In JA – Auxin treatment, the mean root number per bulblet was highest (17.9 number/explant) and root formation rate was maximum (81.14%) on MS medium supplemented with IAA 1.0 mg L-1 + JA 2.0 mg L-1.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3473plant propagationin vitrogrowth regulatorsornamental plantsbulb formation |
| spellingShingle | Selay Doğan Gülat Çağlar Esra Bulunuz Palaz The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey Turkish Journal of Agriculture: Food Science and Technology plant propagation in vitro growth regulators ornamental plants bulb formation |
| title | The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey |
| title_full | The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey |
| title_fullStr | The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey |
| title_full_unstemmed | The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey |
| title_short | The Effect of Different Applications on In vitro Bulb Development of an Endemic Hyacinth Plant (Hyacinthus orientalis L. subsp. chionophyllus Wendelbo) Grown in Turkey |
| title_sort | effect of different applications on in vitro bulb development of an endemic hyacinth plant hyacinthus orientalis l subsp chionophyllus wendelbo grown in turkey |
| topic | plant propagation in vitro growth regulators ornamental plants bulb formation |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3473 |
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