Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone
In this study, eight food by-products were investigated as biosorbent approaches in removing mycotoxin load towards potential dietary inclusion in animal feed. Among these food-derived by-products, grape seed (GSM) and seabuckthorn (SBM) meals showed the most promising binding capacity for Aflatoxin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/1/2 |
| _version_ | 1797543898562691072 |
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| author | Laurentiu Mihai Palade Madalina Ioana Dore Daniela Eliza Marin Mircea Catalin Rotar Ionelia Taranu |
| author_facet | Laurentiu Mihai Palade Madalina Ioana Dore Daniela Eliza Marin Mircea Catalin Rotar Ionelia Taranu |
| author_sort | Laurentiu Mihai Palade |
| collection | DOAJ |
| description | In this study, eight food by-products were investigated as biosorbent approaches in removing mycotoxin load towards potential dietary inclusion in animal feed. Among these food-derived by-products, grape seed (GSM) and seabuckthorn (SBM) meals showed the most promising binding capacity for Aflatoxin B1 (AFB1) and Zearalenone (ZEA), measured as percent of adsorbed mycotoxin. Furthermore, we explored the mycotoxin sequestering potential by screening the effect of time, concentration, temperature and pH. Comparative binding efficacy was addressed by carrying out adsorption experiments in vitro. The highest mycotoxin adsorption was attained using 30 mg of by-product for both GSM (85.9% AFB1 and 83.7% ZEA) and SBM (68% AFB1 and 84.5% ZEA). Optimal settings for the experimental factors were predicted employing the response surface design. GSM was estimated to adsorb AFB1 optimally at a concentration of 29 mg/mL, pH 5.95 and 33.6 °C, and ZEA using 28 mg/mL at pH 5.76 and 31.7 °C. Favorable adsorption of AFB1 was estimated at 37.5 mg of SBM (pH 8.1; 35.6 °C), and of ZEA at 30.2 mg of SBM (pH 5.6; 29.3 °C). Overall, GSM revealed a higher binding capacity compared with SBM. In addition, the two by-products showed different specificity for the binary–mycotoxin system, with SBM having higher affinity towards ZEA than AFB1 (K<sub>f</sub> = 0.418 and 1/n = 0.213 vs. K<sub>f</sub> = 0.217 and 1/n = 0.341) and GSM for AFB1 in comparison with ZEA (K<sub>f</sub> = 0.367 and 1/n = 0.248 vs. K<sub>f</sub> = 0.343 and 1/n = 0.264). In conclusion, this study suggests that GSM and SBM represent viable alternatives to commercial biosorbent products. |
| first_indexed | 2024-03-10T13:52:00Z |
| format | Article |
| id | doaj.art-1f68f273db7c4628826aa0be1058444a |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T13:52:00Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-1f68f273db7c4628826aa0be1058444a2023-11-21T02:01:11ZengMDPI AGToxins2072-66512020-12-01131210.3390/toxins13010002Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and ZearalenoneLaurentiu Mihai Palade0Madalina Ioana Dore1Daniela Eliza Marin2Mircea Catalin Rotar3Ionelia Taranu4National Research Development Institute for Animal Biology and Nutrition, 077015 IBNA Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015 IBNA Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015 IBNA Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015 IBNA Balotesti, RomaniaNational Research Development Institute for Animal Biology and Nutrition, 077015 IBNA Balotesti, RomaniaIn this study, eight food by-products were investigated as biosorbent approaches in removing mycotoxin load towards potential dietary inclusion in animal feed. Among these food-derived by-products, grape seed (GSM) and seabuckthorn (SBM) meals showed the most promising binding capacity for Aflatoxin B1 (AFB1) and Zearalenone (ZEA), measured as percent of adsorbed mycotoxin. Furthermore, we explored the mycotoxin sequestering potential by screening the effect of time, concentration, temperature and pH. Comparative binding efficacy was addressed by carrying out adsorption experiments in vitro. The highest mycotoxin adsorption was attained using 30 mg of by-product for both GSM (85.9% AFB1 and 83.7% ZEA) and SBM (68% AFB1 and 84.5% ZEA). Optimal settings for the experimental factors were predicted employing the response surface design. GSM was estimated to adsorb AFB1 optimally at a concentration of 29 mg/mL, pH 5.95 and 33.6 °C, and ZEA using 28 mg/mL at pH 5.76 and 31.7 °C. Favorable adsorption of AFB1 was estimated at 37.5 mg of SBM (pH 8.1; 35.6 °C), and of ZEA at 30.2 mg of SBM (pH 5.6; 29.3 °C). Overall, GSM revealed a higher binding capacity compared with SBM. In addition, the two by-products showed different specificity for the binary–mycotoxin system, with SBM having higher affinity towards ZEA than AFB1 (K<sub>f</sub> = 0.418 and 1/n = 0.213 vs. K<sub>f</sub> = 0.217 and 1/n = 0.341) and GSM for AFB1 in comparison with ZEA (K<sub>f</sub> = 0.367 and 1/n = 0.248 vs. K<sub>f</sub> = 0.343 and 1/n = 0.264). In conclusion, this study suggests that GSM and SBM represent viable alternatives to commercial biosorbent products.https://www.mdpi.com/2072-6651/13/1/2mycotoxinsfood by-productsgrape seed mealseabuckthorn mealwaste recyclingdecontamination |
| spellingShingle | Laurentiu Mihai Palade Madalina Ioana Dore Daniela Eliza Marin Mircea Catalin Rotar Ionelia Taranu Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone Toxins mycotoxins food by-products grape seed meal seabuckthorn meal waste recycling decontamination |
| title | Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone |
| title_full | Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone |
| title_fullStr | Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone |
| title_full_unstemmed | Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone |
| title_short | Assessment of Food By-Products’ Potential for Simultaneous Binding of Aflatoxin B1 and Zearalenone |
| title_sort | assessment of food by products potential for simultaneous binding of aflatoxin b1 and zearalenone |
| topic | mycotoxins food by-products grape seed meal seabuckthorn meal waste recycling decontamination |
| url | https://www.mdpi.com/2072-6651/13/1/2 |
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