EZmito: a simple and fast tool for multiple mitogenome analyses

Complete mitochondrial genome data are frequently applied to address phylogenetic/phylogeographic issues at different taxonomic levels in ecology and evolution. While sample preparation/sequencing is becoming more and more straightforward thanks to dropping costs for next-generation sequencing (NGS)...

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Main Authors: Claudio Cucini, Chiara Leo, Nicola Iannotti, Sara Boschi, Claudia Brunetti, Joan Pons, Pietro Paolo Fanciulli, Francesco Frati, Antonio Carapelli, Francesco Nardi
Format: Article
Language:English
Published: Taylor & Francis Group 2021-03-01
Series:Mitochondrial DNA. Part B. Resources
Subjects:
Online Access:http://dx.doi.org/10.1080/23802359.2021.1899865
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author Claudio Cucini
Chiara Leo
Nicola Iannotti
Sara Boschi
Claudia Brunetti
Joan Pons
Pietro Paolo Fanciulli
Francesco Frati
Antonio Carapelli
Francesco Nardi
author_facet Claudio Cucini
Chiara Leo
Nicola Iannotti
Sara Boschi
Claudia Brunetti
Joan Pons
Pietro Paolo Fanciulli
Francesco Frati
Antonio Carapelli
Francesco Nardi
author_sort Claudio Cucini
collection DOAJ
description Complete mitochondrial genome data are frequently applied to address phylogenetic/phylogeographic issues at different taxonomic levels in ecology and evolution. While sample preparation/sequencing is becoming more and more straightforward thanks to dropping costs for next-generation sequencing (NGS), data preparation and visualization remains a manually intensive step that may lead to errors if improperly conducted. We have elaborated, and here introduce, EZmito, a simple and intuitive, freely accessible Web Server aimed at automating some of these tasks. EZmito is divided into three main tools: EZpipe that assembles DNA matrices for phylo-mitogenomic analyses; EZskew that calculates genome, strand, and codon nucleotide compositional skews and EZcodon which computes Relative Synonymous Codon Usage statistics as well as amino acid usage frequency over multiple mitogenomes. Output is produced in tabular format as well as publication-quality graphics.
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spelling doaj.art-1f6bcfe9d33045298d8a82f65efe43832023-11-03T14:16:07ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592021-03-01631101110910.1080/23802359.2021.18998651899865EZmito: a simple and fast tool for multiple mitogenome analysesClaudio Cucini0Chiara Leo1Nicola Iannotti2Sara Boschi3Claudia Brunetti4Joan Pons5Pietro Paolo Fanciulli6Francesco Frati7Antonio Carapelli8Francesco Nardi9Department of Life Sciences, University of SienaDepartment of Life Sciences, Imperial College LondonDepartment of Life Sciences, University of SienaDepartment of Life Sciences, University of SienaDepartment of Life Sciences, University of SienaDepartment de Biodiversitat Animal i Microbiana, Institut Mediterrani d’Estudis AvancatsDepartment of Life Sciences, University of SienaDepartment of Life Sciences, University of SienaDepartment of Life Sciences, University of SienaDepartment of Life Sciences, University of SienaComplete mitochondrial genome data are frequently applied to address phylogenetic/phylogeographic issues at different taxonomic levels in ecology and evolution. While sample preparation/sequencing is becoming more and more straightforward thanks to dropping costs for next-generation sequencing (NGS), data preparation and visualization remains a manually intensive step that may lead to errors if improperly conducted. We have elaborated, and here introduce, EZmito, a simple and intuitive, freely accessible Web Server aimed at automating some of these tasks. EZmito is divided into three main tools: EZpipe that assembles DNA matrices for phylo-mitogenomic analyses; EZskew that calculates genome, strand, and codon nucleotide compositional skews and EZcodon which computes Relative Synonymous Codon Usage statistics as well as amino acid usage frequency over multiple mitogenomes. Output is produced in tabular format as well as publication-quality graphics.http://dx.doi.org/10.1080/23802359.2021.1899865web serverphylogenymitogenomenucleotide biasrscu
spellingShingle Claudio Cucini
Chiara Leo
Nicola Iannotti
Sara Boschi
Claudia Brunetti
Joan Pons
Pietro Paolo Fanciulli
Francesco Frati
Antonio Carapelli
Francesco Nardi
EZmito: a simple and fast tool for multiple mitogenome analyses
Mitochondrial DNA. Part B. Resources
web server
phylogeny
mitogenome
nucleotide bias
rscu
title EZmito: a simple and fast tool for multiple mitogenome analyses
title_full EZmito: a simple and fast tool for multiple mitogenome analyses
title_fullStr EZmito: a simple and fast tool for multiple mitogenome analyses
title_full_unstemmed EZmito: a simple and fast tool for multiple mitogenome analyses
title_short EZmito: a simple and fast tool for multiple mitogenome analyses
title_sort ezmito a simple and fast tool for multiple mitogenome analyses
topic web server
phylogeny
mitogenome
nucleotide bias
rscu
url http://dx.doi.org/10.1080/23802359.2021.1899865
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