Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene
Background: Actually, no data on the prevalence of plasmid colistin resistance in Tunisia are available among clinical bacteria. Objectives: This study aimed to investigate the current epidemiology of colistin resistance and the spread of the <i>mcr</i> gene in clinical Gram-negative bac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1390 |
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| author | Sana Ferjani Elaa Maamar Asma Ferjani Khaoula Meftah Hager Battikh Besma Mnif Manel Hamdoun Yosra Chebbi Lamia Kanzari Wafa Achour Olfa Bahri Adenene Hammami Meriam Zribi Hanen Smaoui Ilhem Boutiba-Ben Boubaker |
| author_facet | Sana Ferjani Elaa Maamar Asma Ferjani Khaoula Meftah Hager Battikh Besma Mnif Manel Hamdoun Yosra Chebbi Lamia Kanzari Wafa Achour Olfa Bahri Adenene Hammami Meriam Zribi Hanen Smaoui Ilhem Boutiba-Ben Boubaker |
| author_sort | Sana Ferjani |
| collection | DOAJ |
| description | Background: Actually, no data on the prevalence of plasmid colistin resistance in Tunisia are available among clinical bacteria. Objectives: This study aimed to investigate the current epidemiology of colistin resistance and the spread of the <i>mcr</i> gene in clinical Gram-negative bacteria (GNB) isolated from six Tunisian university hospitals. Methods: A total of 836 GNB strains were inoculated on COL-R agar plates with selective screening agar for the isolation of GNB resistant to colistin. For the selected isolates, <i>mcr</i> genes, beta-lactamases associated-resistance genes and molecular characterisation were screened by PCRs and sequencing. Results: Colistin-resistance was detected in 5.02% (42/836) of the isolates and colistin-resistant isolates harboured an ESBL (<i>bla</i><sub>CTX-M-15</sub>) and/or a carbapenemase (<i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>VIM</sub>) encoding gene in 45.2% of the cases. The <i>mcr</i>-1 gene was detected in four <i>E. coli</i> isolates (0.59%) causing urinary tract infections and all these isolates also contained the <i>bla</i><sub>TEM-1</sub> gene. The <i>bla</i><sub>CTX-M-15</sub> gene was detected in three isolates that also carried the IncY and IncFIB replicons. The genetic environment surrounding the <i>mcr</i>-carrying plasmid indicated the presence of <i>pap-2</i> gene upstream <i>mcr</i>-1 resistance marker with unusual missing of ISApl1 insertion sequence. The Conclusions: This study reports the first description of the <i>mcr</i>-1 gene among clinical <i>E. coli</i> isolates in Tunisia and provides an incentive to conduct routine colistin susceptibility testing in GNB clinical isolates. |
| first_indexed | 2024-03-09T20:50:15Z |
| format | Article |
| id | doaj.art-1f6c1c1bce0e4aec8b96a34e80cb12ff |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:50:15Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-1f6c1c1bce0e4aec8b96a34e80cb12ff2023-11-23T22:35:40ZengMDPI AGAntibiotics2079-63822022-10-011110139010.3390/antibiotics11101390Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> GeneSana Ferjani0Elaa Maamar1Asma Ferjani2Khaoula Meftah3Hager Battikh4Besma Mnif5Manel Hamdoun6Yosra Chebbi7Lamia Kanzari8Wafa Achour9Olfa Bahri10Adenene Hammami11Meriam Zribi12Hanen Smaoui13Ilhem Boutiba-Ben Boubaker14Faculty of Medicine of Tunis, University of Tunis El Manar, LR99ES09, Tunis Rue Djebal Lakhdar 1006, TunisiaFaculty of Medicine of Tunis, University of Tunis El Manar, LR99ES09, Tunis Rue Djebal Lakhdar 1006, TunisiaFaculty of Medicine of Tunis, University of Tunis El Manar, LR99ES09, Tunis Rue Djebal Lakhdar 1006, TunisiaLaboratory of Microbiology, Children’s Hospital of Tunis, Boulevard 9 April, Tunis 1006, TunisiaMicrobiology Laboratory, Rabta University Hospital, Rue Jabbari, Tunis 1007, TunisiaLaboratory of Microbiology, Habib Bourguiba University Hospital, Route de l’Ain, Sfax 3000, TunisiaAziza Othmana Hospital, Laboratoire de Microbiologie-Biochimie, Bab Menara Tunis 1008, TunisiaNational Bone Marrow Transplant Center, Laboratory Ward, Tunis Rue Djebal Lakhdar 1006, TunisiaFaculty of Medicine of Tunis, University of Tunis El Manar, LR99ES09, Tunis Rue Djebal Lakhdar 1006, TunisiaNational Bone Marrow Transplant Center, Laboratory Ward, Tunis Rue Djebal Lakhdar 1006, TunisiaAziza Othmana Hospital, Laboratoire de Microbiologie-Biochimie, Bab Menara Tunis 1008, TunisiaLaboratory of Microbiology, Habib Bourguiba University Hospital, Route de l’Ain, Sfax 3000, TunisiaMicrobiology Laboratory, Rabta University Hospital, Rue Jabbari, Tunis 1007, TunisiaLaboratory of Microbiology, Children’s Hospital of Tunis, Boulevard 9 April, Tunis 1006, TunisiaFaculty of Medicine of Tunis, University of Tunis El Manar, LR99ES09, Tunis Rue Djebal Lakhdar 1006, TunisiaBackground: Actually, no data on the prevalence of plasmid colistin resistance in Tunisia are available among clinical bacteria. Objectives: This study aimed to investigate the current epidemiology of colistin resistance and the spread of the <i>mcr</i> gene in clinical Gram-negative bacteria (GNB) isolated from six Tunisian university hospitals. Methods: A total of 836 GNB strains were inoculated on COL-R agar plates with selective screening agar for the isolation of GNB resistant to colistin. For the selected isolates, <i>mcr</i> genes, beta-lactamases associated-resistance genes and molecular characterisation were screened by PCRs and sequencing. Results: Colistin-resistance was detected in 5.02% (42/836) of the isolates and colistin-resistant isolates harboured an ESBL (<i>bla</i><sub>CTX-M-15</sub>) and/or a carbapenemase (<i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>VIM</sub>) encoding gene in 45.2% of the cases. The <i>mcr</i>-1 gene was detected in four <i>E. coli</i> isolates (0.59%) causing urinary tract infections and all these isolates also contained the <i>bla</i><sub>TEM-1</sub> gene. The <i>bla</i><sub>CTX-M-15</sub> gene was detected in three isolates that also carried the IncY and IncFIB replicons. The genetic environment surrounding the <i>mcr</i>-carrying plasmid indicated the presence of <i>pap-2</i> gene upstream <i>mcr</i>-1 resistance marker with unusual missing of ISApl1 insertion sequence. The Conclusions: This study reports the first description of the <i>mcr</i>-1 gene among clinical <i>E. coli</i> isolates in Tunisia and provides an incentive to conduct routine colistin susceptibility testing in GNB clinical isolates.https://www.mdpi.com/2079-6382/11/10/1390colistin resistanceGram negative bacteria<i>mcr-1</i> genemulti-drug resistant bacteria |
| spellingShingle | Sana Ferjani Elaa Maamar Asma Ferjani Khaoula Meftah Hager Battikh Besma Mnif Manel Hamdoun Yosra Chebbi Lamia Kanzari Wafa Achour Olfa Bahri Adenene Hammami Meriam Zribi Hanen Smaoui Ilhem Boutiba-Ben Boubaker Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene Antibiotics colistin resistance Gram negative bacteria <i>mcr-1</i> gene multi-drug resistant bacteria |
| title | Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene |
| title_full | Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene |
| title_fullStr | Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene |
| title_full_unstemmed | Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene |
| title_short | Tunisian Multicenter Study on the Prevalence of Colistin Resistance in Clinical Isolates of Gram Negative Bacilli: Emergence of <i>Escherichia coli</i> Harbouring the <i>mcr-1</i> Gene |
| title_sort | tunisian multicenter study on the prevalence of colistin resistance in clinical isolates of gram negative bacilli emergence of i escherichia coli i harbouring the i mcr 1 i gene |
| topic | colistin resistance Gram negative bacteria <i>mcr-1</i> gene multi-drug resistant bacteria |
| url | https://www.mdpi.com/2079-6382/11/10/1390 |
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