Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger
This study investigated sweet potato peel waste as the main carbon source and determine the effect of media components and operating conditions on citric acid (CA) production by Aspergillus niger under submerged fermentation. The sweet potato peel waste is of low-cost readily-available and serve as...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227620302921 |
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| author | O.O. Aboyeji J.K. Oloke, PhD A.O. Arinkoola, PhD M.A. Oke, PhD M.M. Ishola, PhD |
| author_facet | O.O. Aboyeji J.K. Oloke, PhD A.O. Arinkoola, PhD M.A. Oke, PhD M.M. Ishola, PhD |
| author_sort | O.O. Aboyeji |
| collection | DOAJ |
| description | This study investigated sweet potato peel waste as the main carbon source and determine the effect of media components and operating conditions on citric acid (CA) production by Aspergillus niger under submerged fermentation. The sweet potato peel waste is of low-cost readily-available and serve as hydrolysate for CA production. Two sets of experiments were performed using experimental design technique. The first set was designed using a low resolution 2-level factorial which was deployed for parameter screening. The other set of experiments was designed using 3-level design algorithm and was deployed for model development and optimization. The results from parameter screening showed that carbon source (CS), nitrogen concentration (NC), fermentation time (FT) and pH were the “heavy hitters” with significant impact on CA production. The developed model is predictive (R2 = 0.948) with optimum CA (3.22 mg/mL) obtained at CS (97.25%), NC (1.25%w/v), FT (7 days), and pH (6.5). This setting was validated experimentally and resulted in CA yield of 4.36 ± 006 mg/ml, representing a 135%-fold increase over the predicted yield. The present investigation shows that sweet potato peels hydrolysate is a viable and sustainable substrate for CA production. |
| first_indexed | 2024-12-16T18:19:11Z |
| format | Article |
| id | doaj.art-1f6d64a4dcbe4ce7ab0f71df856765d6 |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-12-16T18:19:11Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-1f6d64a4dcbe4ce7ab0f71df856765d62022-12-21T22:21:36ZengElsevierScientific African2468-22762020-11-0110e00554Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus nigerO.O. Aboyeji0J.K. Oloke, PhD1A.O. Arinkoola, PhD2M.A. Oke, PhD3M.M. Ishola, PhD4Department of Biology, Kwara State College of Education (Technical), Lafiagi, Nigeria; Department of Pure and Applied Biology, Ladoke Akintola University of Technology (LAUTECH), Ogbomoso, Nigeria; Corresponding author at: Department of Biology, Kwara State College of Education (Technical), Lafiagi, Nigeria.Department of Pure and Applied Biology, Ladoke Akintola University of Technology (LAUTECH), Ogbomoso, NigeriaDepartment of Chemical Engineering, Ladoke Akintola University of Technology (LAUTECH), Ogbomoso, NigeriaDepartment of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, NigeriaDepartment of Environmental Engineering Göteborg Energi, Gothenburg, SwedenThis study investigated sweet potato peel waste as the main carbon source and determine the effect of media components and operating conditions on citric acid (CA) production by Aspergillus niger under submerged fermentation. The sweet potato peel waste is of low-cost readily-available and serve as hydrolysate for CA production. Two sets of experiments were performed using experimental design technique. The first set was designed using a low resolution 2-level factorial which was deployed for parameter screening. The other set of experiments was designed using 3-level design algorithm and was deployed for model development and optimization. The results from parameter screening showed that carbon source (CS), nitrogen concentration (NC), fermentation time (FT) and pH were the “heavy hitters” with significant impact on CA production. The developed model is predictive (R2 = 0.948) with optimum CA (3.22 mg/mL) obtained at CS (97.25%), NC (1.25%w/v), FT (7 days), and pH (6.5). This setting was validated experimentally and resulted in CA yield of 4.36 ± 006 mg/ml, representing a 135%-fold increase over the predicted yield. The present investigation shows that sweet potato peels hydrolysate is a viable and sustainable substrate for CA production.http://www.sciencedirect.com/science/article/pii/S2468227620302921Aspergillus nigerCitric acidOptimizationSubmerged fermentationSweet potato peelHydrolysate |
| spellingShingle | O.O. Aboyeji J.K. Oloke, PhD A.O. Arinkoola, PhD M.A. Oke, PhD M.M. Ishola, PhD Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger Scientific African Aspergillus niger Citric acid Optimization Submerged fermentation Sweet potato peel Hydrolysate |
| title | Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger |
| title_full | Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger |
| title_fullStr | Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger |
| title_full_unstemmed | Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger |
| title_short | Optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by Aspergillus niger |
| title_sort | optimization of media components and fermentation conditions for citric acid production from sweet potato peel starch hydrolysate by aspergillus niger |
| topic | Aspergillus niger Citric acid Optimization Submerged fermentation Sweet potato peel Hydrolysate |
| url | http://www.sciencedirect.com/science/article/pii/S2468227620302921 |
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