Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/347 |
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| author | Maria Cristina Queiroga Marta Laranjo Nara Andrade Mariana Marques Ana Rodrigues Costa Célia Maria Antunes |
| author_facet | Maria Cristina Queiroga Marta Laranjo Nara Andrade Mariana Marques Ana Rodrigues Costa Célia Maria Antunes |
| author_sort | Maria Cristina Queiroga |
| collection | DOAJ |
| description | Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its antimicrobial and antibiofilm activities. To assess propolis for topical medical use, toxicological studies were also performed. A Portuguese 70% propolis ethanolic extract was chemically evaluated and studied for antimicrobial activity on staphylococcal field isolates (n = 137) and antibiofilm action (n = 45). Cell toxicological assessment was performed using keratinocytes and fibroblasts. Pinobanksin, chrysin, acacetin, apigenin, pinocembrin, and kaempferol-dimethyl-ether were detected. All 137 isolates were susceptible to 6.68 mg/mL or lower propolis concentration (80% isolates were susceptible to <1 mg/mL). The mean percentage of biofilm inhibition was 71%, and biofilm disruption was 88.5%. Propolis (<1 mg/mL) was well-tolerated by fibroblasts and moderately tolerated by keratinocytes. The combined antimicrobial and antibiofilm effect of propolis, together with its low toxicity to connective tissue and epithelial cells, suggests a good applicability for topical antibacterial treatment. Therefore, propolis seems to be a good alternative to antimicrobials for the treatment of infections with <i>Staphylococcus</i> spp. that deserves to be evaluated in vivo. |
| first_indexed | 2024-03-11T09:14:45Z |
| format | Article |
| id | doaj.art-1f6ed2caada24569a9248e6289d1c887 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:14:45Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-1f6ed2caada24569a9248e6289d1c8872023-11-16T18:43:44ZengMDPI AGAntibiotics2079-63822023-02-0112234710.3390/antibiotics12020347Antimicrobial, Antibiofilm and Toxicological Assessment of PropolisMaria Cristina Queiroga0Marta Laranjo1Nara Andrade2Mariana Marques3Ana Rodrigues Costa4Célia Maria Antunes5MED–Mediterranean Institute for Agriculture, Environment and Development & CHANGE–Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED–Mediterranean Institute for Agriculture, Environment and Development & CHANGE–Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalUniversidade Paulista, Campus Petrolina-PE, Av. Barão do Rio Branco, 700-862 - Centro, Petrolina - PE, CEP: 56304-260, BrazilInstituto de Ciências da Terra, ICT, Universidade de Évora, 7006-554 Évora, PortugalInstituto de Ciências da Terra, ICT, Universidade de Évora, 7006-554 Évora, PortugalInstituto de Ciências da Terra, ICT, Universidade de Évora, 7006-554 Évora, PortugalAntimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its antimicrobial and antibiofilm activities. To assess propolis for topical medical use, toxicological studies were also performed. A Portuguese 70% propolis ethanolic extract was chemically evaluated and studied for antimicrobial activity on staphylococcal field isolates (n = 137) and antibiofilm action (n = 45). Cell toxicological assessment was performed using keratinocytes and fibroblasts. Pinobanksin, chrysin, acacetin, apigenin, pinocembrin, and kaempferol-dimethyl-ether were detected. All 137 isolates were susceptible to 6.68 mg/mL or lower propolis concentration (80% isolates were susceptible to <1 mg/mL). The mean percentage of biofilm inhibition was 71%, and biofilm disruption was 88.5%. Propolis (<1 mg/mL) was well-tolerated by fibroblasts and moderately tolerated by keratinocytes. The combined antimicrobial and antibiofilm effect of propolis, together with its low toxicity to connective tissue and epithelial cells, suggests a good applicability for topical antibacterial treatment. Therefore, propolis seems to be a good alternative to antimicrobials for the treatment of infections with <i>Staphylococcus</i> spp. that deserves to be evaluated in vivo.https://www.mdpi.com/2079-6382/12/2/347propolisantimicrobialantibiofilmtoxicological<i>Staphylococcus</i>fibroblast |
| spellingShingle | Maria Cristina Queiroga Marta Laranjo Nara Andrade Mariana Marques Ana Rodrigues Costa Célia Maria Antunes Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis Antibiotics propolis antimicrobial antibiofilm toxicological <i>Staphylococcus</i> fibroblast |
| title | Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis |
| title_full | Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis |
| title_fullStr | Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis |
| title_full_unstemmed | Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis |
| title_short | Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis |
| title_sort | antimicrobial antibiofilm and toxicological assessment of propolis |
| topic | propolis antimicrobial antibiofilm toxicological <i>Staphylococcus</i> fibroblast |
| url | https://www.mdpi.com/2079-6382/12/2/347 |
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