Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens
This study aimed to determine the effect of the addition of oat hull (0–3%) and high amounts of cereal grains to the diet of broiler chickens in terms of the development of the upper gastrointestinal tract, individual microbial counts in the crop and ileum, and the effect of dietary fibre fractions...
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MDPI AG
2022-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2721 |
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| author | Patrycja Wróblewska Tomasz Hikawczuk Kamil Sierżant Andrzej Wiliczkiewicz Anna Szuba-Trznadel |
| author_facet | Patrycja Wróblewska Tomasz Hikawczuk Kamil Sierżant Andrzej Wiliczkiewicz Anna Szuba-Trznadel |
| author_sort | Patrycja Wróblewska |
| collection | DOAJ |
| description | This study aimed to determine the effect of the addition of oat hull (0–3%) and high amounts of cereal grains to the diet of broiler chickens in terms of the development of the upper gastrointestinal tract, individual microbial counts in the crop and ileum, and the effect of dietary fibre fractions on microbial changes from the beak to the ileum. In the 28 d trial, 162 one-day-old Hubbard Flex male chickens with an average body weight of 44.5 g were randomly allocated to 27 metabolic cages. The experiment consisted of a randomised, one factorial ANCOVA design composed of a covariate with two ANOVA factorial designs containing nine treatments (3 × 3): three sources of cereal grains (maize, wheat, and barley, with a minimum amount of 500 g · kg<sup>−1</sup>, each with nine replications) and three levels of oat hull (0, 1, and 3%, each with nine replications). At the end of the study, 81 chickens (9 in each treatment) were slaughtered to determine the weight of the individual organs and characterise the intestinal microbiota. The application of 3% oat hull to the cereal diets increased the weight of the proventriculus and the gizzard (6.3 and 27.3 g, respectively) in comparison to diets without the addition of this structural component (6.0 and 23.7 g). Higher crop counts of total aerobic microbes (6.29 log CFU · g<sup>−1</sup>) and <i>Lactobacillus</i> spp. (4.05 log CFU · g<sup>−1</sup>) were observed in diets containing wheat grain compared with maize (4.62 and 3.55 log CFU · g<sup>−1</sup>, respectively). The main reason for the microorganism’s growth (<i>p</i> < 0.05) was the amount of soluble dietary fibre and hemicelluloses present in the diet: total aerobic microbial count (respectively r = 0.918 and r = 0.816) and <i>Lactobacillus</i> spp. (respectively r = 0.416 and r = 0.442). Barley diets decreased (<i>p</i> < 0.05) <i>E. coli</i> counts in the ileum (2.69 log CFU · g<sup>−1</sup>) vs. maize and wheat diets (3.41 and 3.45 log CFU · g<sup>−1</sup>, respectively), mainly due to the increase in the amount of insoluble dietary fibre in the diet (r = −0.462). Reduced total yeast and mould counts in the ileum were also observed (<i>p</i> < 0.05) in connection with the presence of soluble dietary fibre and hemicelluloses in diets (r = −0.397 and −0.398, respectively). |
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| spelling | doaj.art-1f710532805e4c358436e50ae18b1a2c2023-11-23T19:38:41ZengMDPI AGAnimals2076-26152022-10-011219272110.3390/ani12192721Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler ChickensPatrycja Wróblewska0Tomasz Hikawczuk1Kamil Sierżant2Andrzej Wiliczkiewicz3Anna Szuba-Trznadel4Department of Animal Nutrition and Feed Science, Wroclaw University of Environmental and Life Sciences, 38 c Chełmońskiego Street, 51-630 Wrocław, PolandStatistical Analysis Centre, Wroclaw Medical University, 2-6 Marcinkowskiego Street, 50-368 Wrocław, PolandDepartment of Animal Nutrition and Feed Science, Wroclaw University of Environmental and Life Sciences, 38 c Chełmońskiego Street, 51-630 Wrocław, PolandDepartment of Animal Nutrition and Feed Science, Wroclaw University of Environmental and Life Sciences, 38 c Chełmońskiego Street, 51-630 Wrocław, PolandDepartment of Animal Nutrition and Feed Science, Wroclaw University of Environmental and Life Sciences, 38 c Chełmońskiego Street, 51-630 Wrocław, PolandThis study aimed to determine the effect of the addition of oat hull (0–3%) and high amounts of cereal grains to the diet of broiler chickens in terms of the development of the upper gastrointestinal tract, individual microbial counts in the crop and ileum, and the effect of dietary fibre fractions on microbial changes from the beak to the ileum. In the 28 d trial, 162 one-day-old Hubbard Flex male chickens with an average body weight of 44.5 g were randomly allocated to 27 metabolic cages. The experiment consisted of a randomised, one factorial ANCOVA design composed of a covariate with two ANOVA factorial designs containing nine treatments (3 × 3): three sources of cereal grains (maize, wheat, and barley, with a minimum amount of 500 g · kg<sup>−1</sup>, each with nine replications) and three levels of oat hull (0, 1, and 3%, each with nine replications). At the end of the study, 81 chickens (9 in each treatment) were slaughtered to determine the weight of the individual organs and characterise the intestinal microbiota. The application of 3% oat hull to the cereal diets increased the weight of the proventriculus and the gizzard (6.3 and 27.3 g, respectively) in comparison to diets without the addition of this structural component (6.0 and 23.7 g). Higher crop counts of total aerobic microbes (6.29 log CFU · g<sup>−1</sup>) and <i>Lactobacillus</i> spp. (4.05 log CFU · g<sup>−1</sup>) were observed in diets containing wheat grain compared with maize (4.62 and 3.55 log CFU · g<sup>−1</sup>, respectively). The main reason for the microorganism’s growth (<i>p</i> < 0.05) was the amount of soluble dietary fibre and hemicelluloses present in the diet: total aerobic microbial count (respectively r = 0.918 and r = 0.816) and <i>Lactobacillus</i> spp. (respectively r = 0.416 and r = 0.442). Barley diets decreased (<i>p</i> < 0.05) <i>E. coli</i> counts in the ileum (2.69 log CFU · g<sup>−1</sup>) vs. maize and wheat diets (3.41 and 3.45 log CFU · g<sup>−1</sup>, respectively), mainly due to the increase in the amount of insoluble dietary fibre in the diet (r = −0.462). Reduced total yeast and mould counts in the ileum were also observed (<i>p</i> < 0.05) in connection with the presence of soluble dietary fibre and hemicelluloses in diets (r = −0.397 and −0.398, respectively).https://www.mdpi.com/2076-2615/12/19/2721dietary fibreoat hullbroiler chickensmicrobial statuscrop |
| spellingShingle | Patrycja Wróblewska Tomasz Hikawczuk Kamil Sierżant Andrzej Wiliczkiewicz Anna Szuba-Trznadel Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens Animals dietary fibre oat hull broiler chickens microbial status crop |
| title | Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens |
| title_full | Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens |
| title_fullStr | Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens |
| title_full_unstemmed | Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens |
| title_short | Effect of Oat Hull as a Source of Insoluble Dietary Fibre on Changes in the Microbial Status of Gastrointestinal Tract in Broiler Chickens |
| title_sort | effect of oat hull as a source of insoluble dietary fibre on changes in the microbial status of gastrointestinal tract in broiler chickens |
| topic | dietary fibre oat hull broiler chickens microbial status crop |
| url | https://www.mdpi.com/2076-2615/12/19/2721 |
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