Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition
Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2019-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700097M.pdf |
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| author | Magić Marko Čolović Božana Jokanović Vukoman Vasilijić Saša Marković Milan Vučević Dragana Rudolf Rebeka Čolić Snježana Čolić Miodrag |
| author_facet | Magić Marko Čolović Božana Jokanović Vukoman Vasilijić Saša Marković Milan Vučević Dragana Rudolf Rebeka Čolić Snježana Čolić Miodrag |
| author_sort | Magić Marko |
| collection | DOAJ |
| description | Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightlymoderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. ON 175102 and Grant no. 172026] |
| first_indexed | 2024-04-12T10:25:43Z |
| format | Article |
| id | doaj.art-1f7199e830944b45996baea27ef1526a |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-04-12T10:25:43Z |
| publishDate | 2019-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-1f7199e830944b45996baea27ef1526a2022-12-22T03:36:58ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202019-01-0176549250110.2298/VSP170809097M0042-84501700097MCytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet depositionMagić Marko0Čolović Božana1Jokanović Vukoman2Vasilijić Saša3Marković Milan4Vučević Dragana5Rudolf Rebeka6Čolić Snježana7Čolić Miodrag8University of Belgrade, School of Dentistry, Department of Oral Surgery, Belgrade, SerbiaUniversity of Belgrade, Vinča Institute of Nuclear Sciences, Belgrade, SerbiaUniversity of Belgrade, Vinča Institute of Nuclear Sciences, Belgrade, SerbiaMilitary Medical Academy, Institute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaMilitary Medical Academy, Institute for Medical Research, Belgrade, Serbia Military Medical Academy, Institute for Medical Research, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaUniversity of Maribor, Faculty of Mechanical Engineering, Maribor, SloveniaUniversity of Belgrade, School of Dentistry, Department of Oral Surgery, Belgrade, SerbiaMilitary Medical Academy, Faculty of Medicine, Institute for Medical Research, Belgrade + Institute for the Application of Nuclear Energy, BelgradeBackground/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightlymoderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. ON 175102 and Grant no. 172026]http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700097M.pdfTi6Al4V alloyhydroxyapatite coatingplasma jet depositioncytotoxicityalloy conditioning |
| spellingShingle | Magić Marko Čolović Božana Jokanović Vukoman Vasilijić Saša Marković Milan Vučević Dragana Rudolf Rebeka Čolić Snježana Čolić Miodrag Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition Vojnosanitetski Pregled Ti6Al4V alloy hydroxyapatite coating plasma jet deposition cytotoxicity alloy conditioning |
| title | Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| title_full | Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| title_fullStr | Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| title_full_unstemmed | Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| title_short | Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| title_sort | cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition |
| topic | Ti6Al4V alloy hydroxyapatite coating plasma jet deposition cytotoxicity alloy conditioning |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700097M.pdf |
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