Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers
Carbapenem-resistant Enterobacteriaceae (CRE) has been considered a serious global threat, but carbapenem resistance remains relatively uncommon in E. cloacae, especially in China. The aim of this study was to characterize carbapenem-resistant E. cloacae (CR-ECL) isolates from 2012 to 2016 in Southw...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.00658/full |
| _version_ | 1811193021923852288 |
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| author | Xiaojiong Jia Wei Dai Weijia Ma Jinrong Yan Jianchun He Shuang Li Congya Li Shuangshuang Yang Xiuyu Xu Shan Sun Jing Shi Liping Zhang |
| author_facet | Xiaojiong Jia Wei Dai Weijia Ma Jinrong Yan Jianchun He Shuang Li Congya Li Shuangshuang Yang Xiuyu Xu Shan Sun Jing Shi Liping Zhang |
| author_sort | Xiaojiong Jia |
| collection | DOAJ |
| description | Carbapenem-resistant Enterobacteriaceae (CRE) has been considered a serious global threat, but carbapenem resistance remains relatively uncommon in E. cloacae, especially in China. The aim of this study was to characterize carbapenem-resistant E. cloacae (CR-ECL) isolates from 2012 to 2016 in Southwest China. Our study revealed that 20 (15.2%) of the 132 CR-ECL isolates obtained from patients were identified as NDM-1, with most isolates carrying the IncFIIA plasmids. Notably, we initially observed that the E. cloacae strain co-harbored NDM-1 and IMP-8 carbapenemases simultaneously. Analysis of the genetic environment of these two genes has revealed that the highly conserved regions (blaNDM-1-bleMBL-trpF-tat) are associated with the dissemination of NDM-1, while IS26, intI1, and tniC could be involved in the spread of IMP-8. Molecular epidemiology studies showed the nosocomial outbreak caused by NDM-1-producing E. cloacae ST88. Transferring from another hospital and previous carbapenem exposure were identified as independent risk factors for the acquisition of NDM-1-producing E. cloacae. These findings emphasize the need for intensive surveillance and precautions to monitor the further spread of NDM-1 in China. |
| first_indexed | 2024-04-12T00:01:24Z |
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| id | doaj.art-1f71b4e6b3b1435cbaee9eea6e835266 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T00:01:24Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1f71b4e6b3b1435cbaee9eea6e8352662022-12-22T03:56:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-04-01910.3389/fmicb.2018.00658325155Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-ProducersXiaojiong JiaWei DaiWeijia MaJinrong YanJianchun HeShuang LiCongya LiShuangshuang YangXiuyu XuShan SunJing ShiLiping ZhangCarbapenem-resistant Enterobacteriaceae (CRE) has been considered a serious global threat, but carbapenem resistance remains relatively uncommon in E. cloacae, especially in China. The aim of this study was to characterize carbapenem-resistant E. cloacae (CR-ECL) isolates from 2012 to 2016 in Southwest China. Our study revealed that 20 (15.2%) of the 132 CR-ECL isolates obtained from patients were identified as NDM-1, with most isolates carrying the IncFIIA plasmids. Notably, we initially observed that the E. cloacae strain co-harbored NDM-1 and IMP-8 carbapenemases simultaneously. Analysis of the genetic environment of these two genes has revealed that the highly conserved regions (blaNDM-1-bleMBL-trpF-tat) are associated with the dissemination of NDM-1, while IS26, intI1, and tniC could be involved in the spread of IMP-8. Molecular epidemiology studies showed the nosocomial outbreak caused by NDM-1-producing E. cloacae ST88. Transferring from another hospital and previous carbapenem exposure were identified as independent risk factors for the acquisition of NDM-1-producing E. cloacae. These findings emphasize the need for intensive surveillance and precautions to monitor the further spread of NDM-1 in China.https://www.frontiersin.org/article/10.3389/fmicb.2018.00658/fullcarbapenemsresistanceE. cloacaeoutbreak investigationrisk factor |
| spellingShingle | Xiaojiong Jia Wei Dai Weijia Ma Jinrong Yan Jianchun He Shuang Li Congya Li Shuangshuang Yang Xiuyu Xu Shan Sun Jing Shi Liping Zhang Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers Frontiers in Microbiology carbapenems resistance E. cloacae outbreak investigation risk factor |
| title | Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers |
| title_full | Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers |
| title_fullStr | Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers |
| title_full_unstemmed | Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers |
| title_short | Carbapenem-Resistant E. cloacae in Southwest China: Molecular Analysis of Resistance and Risk Factors for Infections Caused by NDM-1-Producers |
| title_sort | carbapenem resistant e cloacae in southwest china molecular analysis of resistance and risk factors for infections caused by ndm 1 producers |
| topic | carbapenems resistance E. cloacae outbreak investigation risk factor |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.00658/full |
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