Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels
Evidence suggests that arbuscular mycorrhizal fungi (AMF) may promote the growth of woody plants. However, the effects of AMF on nitrogen (N) metabolism in plants, especially trees, and its regulatory mechanism are rarely reported. Here, the effects of AMF inoculation on the growth and N nutrition s...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1138184/full |
| _version_ | 1797896516975722496 |
|---|---|
| author | Wei Chen Xueli Mou Panpan Meng Juan Chen Xiaan Tang Guihua Meng Kexu Xin Yi Zhang Chunyan Wang |
| author_facet | Wei Chen Xueli Mou Panpan Meng Juan Chen Xiaan Tang Guihua Meng Kexu Xin Yi Zhang Chunyan Wang |
| author_sort | Wei Chen |
| collection | DOAJ |
| description | Evidence suggests that arbuscular mycorrhizal fungi (AMF) may promote the growth of woody plants. However, the effects of AMF on nitrogen (N) metabolism in plants, especially trees, and its regulatory mechanism are rarely reported. Here, the effects of AMF inoculation on the growth and N nutrition status of Catalpa bungei under different N levels were reported. Three N levels (low, medium, high) and two mycorrhizal inoculation treatments (inoculation with Rhizophagus intraradices or not) were used with factorial design. The results showed that medium N could significantly improve the physiological metabolism and growth of C. bungei seedlings. However, when N was excessive, growth was significantly inhibited whether inoculated AMF or not. Compared with non-inoculated treatments, AMF inoculation could promote the absorption of N and P, improve photosynthesis under low to medium N levels, thus promoting the growth of seedlings. AMF changed the biomass allocation in seedlings by reducing the stem mass ratio and root/shoot ratio, and increasing the leaf mass ratio. At medium N levels, compared with non-inoculated treatment, AMF inoculation could significantly promote root growth by changing root hormone levels and improving root architecture and root activity. Under N addition, AMF inoculation could improve the absorption and assimilation of N by regulating the expression of key enzyme genes of N metabolism and nitrate transporter genes (NRT2.4, NRT2.5, NRT2.7) in roots, and enhancing the activities of the key enzyme of N metabolism. This study may provide a reference for the application of AMF in the cultivation and afforestation technology of C. bungei in Northwest China. |
| first_indexed | 2024-04-10T07:43:01Z |
| format | Article |
| id | doaj.art-1f76c6cc443744c8bbf4b47d751c7e56 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T07:43:01Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1f76c6cc443744c8bbf4b47d751c7e562023-02-23T11:15:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11381841138184Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levelsWei ChenXueli MouPanpan MengJuan ChenXiaan TangGuihua MengKexu XinYi ZhangChunyan WangEvidence suggests that arbuscular mycorrhizal fungi (AMF) may promote the growth of woody plants. However, the effects of AMF on nitrogen (N) metabolism in plants, especially trees, and its regulatory mechanism are rarely reported. Here, the effects of AMF inoculation on the growth and N nutrition status of Catalpa bungei under different N levels were reported. Three N levels (low, medium, high) and two mycorrhizal inoculation treatments (inoculation with Rhizophagus intraradices or not) were used with factorial design. The results showed that medium N could significantly improve the physiological metabolism and growth of C. bungei seedlings. However, when N was excessive, growth was significantly inhibited whether inoculated AMF or not. Compared with non-inoculated treatments, AMF inoculation could promote the absorption of N and P, improve photosynthesis under low to medium N levels, thus promoting the growth of seedlings. AMF changed the biomass allocation in seedlings by reducing the stem mass ratio and root/shoot ratio, and increasing the leaf mass ratio. At medium N levels, compared with non-inoculated treatment, AMF inoculation could significantly promote root growth by changing root hormone levels and improving root architecture and root activity. Under N addition, AMF inoculation could improve the absorption and assimilation of N by regulating the expression of key enzyme genes of N metabolism and nitrate transporter genes (NRT2.4, NRT2.5, NRT2.7) in roots, and enhancing the activities of the key enzyme of N metabolism. This study may provide a reference for the application of AMF in the cultivation and afforestation technology of C. bungei in Northwest China.https://www.frontiersin.org/articles/10.3389/fpls.2023.1138184/fullwoody plantRhizophagus intraradicesgrowth characteristicsphysiological performancenitrogen nutritiongene expression |
| spellingShingle | Wei Chen Xueli Mou Panpan Meng Juan Chen Xiaan Tang Guihua Meng Kexu Xin Yi Zhang Chunyan Wang Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels Frontiers in Plant Science woody plant Rhizophagus intraradices growth characteristics physiological performance nitrogen nutrition gene expression |
| title | Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels |
| title_full | Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels |
| title_fullStr | Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels |
| title_full_unstemmed | Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels |
| title_short | Effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of Catalpa bungei C.A.Mey. under different nitrogen levels |
| title_sort | effects of arbuscular mycorrhizal fungus inoculation on the growth and nitrogen metabolism of catalpa bungei c a mey under different nitrogen levels |
| topic | woody plant Rhizophagus intraradices growth characteristics physiological performance nitrogen nutrition gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1138184/full |
| work_keys_str_mv | AT weichen effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT xuelimou effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT panpanmeng effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT juanchen effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT xiaantang effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT guihuameng effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT kexuxin effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT yizhang effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels AT chunyanwang effectsofarbuscularmycorrhizalfungusinoculationonthegrowthandnitrogenmetabolismofcatalpabungeicameyunderdifferentnitrogenlevels |