The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture
Background & Objective: It has been shown that glial activation has important role in the pathophysiology of Alzheimer’s disease. S100B is an astrocyte specific factor with deleterious effects on the neuronal and non-neuronal cells in the central nervous system. Arundic acid is an agent that inh...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2019-07-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1760-en.pdf |
| _version_ | 1797679609314017280 |
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| author | Mahshid Hosseini Mojtaba Keshavarz Mahmood Vessal |
| author_facet | Mahshid Hosseini Mojtaba Keshavarz Mahmood Vessal |
| author_sort | Mahshid Hosseini |
| collection | DOAJ |
| description | Background & Objective: It has been shown that glial activation has important role in the pathophysiology of Alzheimer’s disease. S100B is an astrocyte specific factor with deleterious effects on the neuronal and non-neuronal cells in the central nervous system. Arundic acid is an agent that inhibits the secretion and production of S100B in astrocytes. Therefore, we aimed to evaluate the contribution of S100B in the cyto-protective effects of Arundic acid against beta-amyloid in 1321N1 astrocyte cell culture.
Materials & Methods: Human astrocyte cells (1321N1) were treated with beta-amyloid (200 μM) and / or Arundic acid (50 μM) for 24 hours. Cell viability was measured using the MTT (3, 4, 5-dimethylthiazole-2, 5-diphenyl tetrazolium bromide) method. The S100B protein level was measured by the ELISA method.
Results: Beta-amyloid treatment reduced cell survival compared to the control-treated groups. In contrast, the addition of Arundic acid to beta-amyloid suppressed the beta-amyloid-induced cell death. Beta-amyloid also increased the S100B protein level. However, Arundic acid prevented the rise of S100B protein level induced by beta-amyloid.
Conclusion: The reduction of S100B protein secretion may be involved in the protective effects of Arundic acid against the beta-amyloid induced Glio-toxicity in the astrocyte culture. |
| first_indexed | 2024-03-11T23:17:16Z |
| format | Article |
| id | doaj.art-1f770e236f854681b1959e0c869a0941 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:16Z |
| publishDate | 2019-07-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-1f770e236f854681b1959e0c869a09412023-09-20T21:26:22ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232019-07-019213991407The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes CultureMahshid Hosseini0Mojtaba Keshavarz1Mahmood Vessal2 Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran Shiraz Neuroscience Research Center, Shiraz University of Medical sciences, Shiraz, Iran Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran Background & Objective: It has been shown that glial activation has important role in the pathophysiology of Alzheimer’s disease. S100B is an astrocyte specific factor with deleterious effects on the neuronal and non-neuronal cells in the central nervous system. Arundic acid is an agent that inhibits the secretion and production of S100B in astrocytes. Therefore, we aimed to evaluate the contribution of S100B in the cyto-protective effects of Arundic acid against beta-amyloid in 1321N1 astrocyte cell culture. Materials & Methods: Human astrocyte cells (1321N1) were treated with beta-amyloid (200 μM) and / or Arundic acid (50 μM) for 24 hours. Cell viability was measured using the MTT (3, 4, 5-dimethylthiazole-2, 5-diphenyl tetrazolium bromide) method. The S100B protein level was measured by the ELISA method. Results: Beta-amyloid treatment reduced cell survival compared to the control-treated groups. In contrast, the addition of Arundic acid to beta-amyloid suppressed the beta-amyloid-induced cell death. Beta-amyloid also increased the S100B protein level. However, Arundic acid prevented the rise of S100B protein level induced by beta-amyloid. Conclusion: The reduction of S100B protein secretion may be involved in the protective effects of Arundic acid against the beta-amyloid induced Glio-toxicity in the astrocyte culture.http://jabs.fums.ac.ir/article-1-1760-en.pdfalzheimer diseaseamyloid beta-peptidess-100 calcium binding protein beta subunitarundic acidastrocytes |
| spellingShingle | Mahshid Hosseini Mojtaba Keshavarz Mahmood Vessal The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture Journal of Advanced Biomedical Sciences alzheimer disease amyloid beta-peptides s-100 calcium binding protein beta subunit arundic acid astrocytes |
| title | The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture |
| title_full | The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture |
| title_fullStr | The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture |
| title_full_unstemmed | The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture |
| title_short | The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture |
| title_sort | contribution of s100b suppression by arundic acid to the inhibition of the glio toxicity induced by beta amyloid in an astrocytes culture |
| topic | alzheimer disease amyloid beta-peptides s-100 calcium binding protein beta subunit arundic acid astrocytes |
| url | http://jabs.fums.ac.ir/article-1-1760-en.pdf |
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