Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection
Rice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/12/12/1387 |
| _version_ | 1797545732221173760 |
|---|---|
| author | Mingjun Li Xi Sun Dianping Di Aihong Zhang Ling Qing Tao Zhou Hongqin Miao Zaifeng Fan |
| author_facet | Mingjun Li Xi Sun Dianping Di Aihong Zhang Ling Qing Tao Zhou Hongqin Miao Zaifeng Fan |
| author_sort | Mingjun Li |
| collection | DOAJ |
| description | Rice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried out using the viral P8 protein as a bait. ZmAKINβγ-1 and <i>ZmAKINβγ-2</i> (βγ subunit of <i>Arabidopsis</i> SNF1 kinase homolog in maize) possessing high sequence similarities (encoded by two gene copies) were identified as interaction partners. Their interactions with P8 were confirmed in both <i>Nicotiana benthamiana</i> cells and maize protoplasts by bimolecular fluorescence complementation assay. The accumulation levels of <i>ZmAKINβγ</i> mRNAs were upregulated at the stage of the viral symptoms beginning to appear and then downregulated. ZmAKINβγs are putative regulatory subunits of the SnRK1 complex, a core regulator for energy homeostasis. Knockdown of <i>ZmAKINβγ</i>s in maize regulated the expression levels of the genes involved in sugar synthesis or degradation, and also the contents of both glucose and sucrose. Importantly, downregulation of <i>ZmAKINβγ</i>s expressions facilitated the accumulation of RBSDV in maize. These results implicate a role of <i>ZmAKINβγ</i>s in the regulation of primary carbohydrate metabolism, and in the defense against RBSDV infection. |
| first_indexed | 2024-03-10T14:20:09Z |
| format | Article |
| id | doaj.art-1f7d06baa548481caa64b9f526e3e308 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T14:20:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1f7d06baa548481caa64b9f526e3e3082023-11-20T23:26:46ZengMDPI AGViruses1999-49152020-12-011212138710.3390/v12121387Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral InfectionMingjun Li0Xi Sun1Dianping Di2Aihong Zhang3Ling Qing4Tao Zhou5Hongqin Miao6Zaifeng Fan7State Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaPlant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, ChinaPlant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaPlant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, ChinaState Key Laboratory of Agro-Biotechnology and Key Laboratory of Pest Monitoring and Green Management-MOA, China Agricultural University, Beijing 100193, ChinaRice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried out using the viral P8 protein as a bait. ZmAKINβγ-1 and <i>ZmAKINβγ-2</i> (βγ subunit of <i>Arabidopsis</i> SNF1 kinase homolog in maize) possessing high sequence similarities (encoded by two gene copies) were identified as interaction partners. Their interactions with P8 were confirmed in both <i>Nicotiana benthamiana</i> cells and maize protoplasts by bimolecular fluorescence complementation assay. The accumulation levels of <i>ZmAKINβγ</i> mRNAs were upregulated at the stage of the viral symptoms beginning to appear and then downregulated. ZmAKINβγs are putative regulatory subunits of the SnRK1 complex, a core regulator for energy homeostasis. Knockdown of <i>ZmAKINβγ</i>s in maize regulated the expression levels of the genes involved in sugar synthesis or degradation, and also the contents of both glucose and sucrose. Importantly, downregulation of <i>ZmAKINβγ</i>s expressions facilitated the accumulation of RBSDV in maize. These results implicate a role of <i>ZmAKINβγ</i>s in the regulation of primary carbohydrate metabolism, and in the defense against RBSDV infection.https://www.mdpi.com/1999-4915/12/12/1387rice black streaked dwarf virusP8 proteinZmAKINβγprotein interactionviral accumulationprimary carbohydrate metabolism |
| spellingShingle | Mingjun Li Xi Sun Dianping Di Aihong Zhang Ling Qing Tao Zhou Hongqin Miao Zaifeng Fan Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection Viruses rice black streaked dwarf virus P8 protein ZmAKINβγ protein interaction viral accumulation primary carbohydrate metabolism |
| title | Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection |
| title_full | Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection |
| title_fullStr | Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection |
| title_full_unstemmed | Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection |
| title_short | Maize AKINβγ Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection |
| title_sort | maize akinβγ proteins interact with p8 of rice black streaked dwarf virus and inhibit viral infection |
| topic | rice black streaked dwarf virus P8 protein ZmAKINβγ protein interaction viral accumulation primary carbohydrate metabolism |
| url | https://www.mdpi.com/1999-4915/12/12/1387 |
| work_keys_str_mv | AT mingjunli maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT xisun maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT dianpingdi maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT aihongzhang maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT lingqing maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT taozhou maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT hongqinmiao maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection AT zaifengfan maizeakinbgproteinsinteractwithp8ofriceblackstreakeddwarfvirusandinhibitviralinfection |