Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons
Apolipoprotein E (apoE) has been confirmed as a risk factor for late-onset Alzheimer's disease (AD) and is associated with neurofibrillary tangles and senile plaques, the microscopic pathological characteristics of AD. There has been no direct evidence that human central nervous system neurons...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1998-10-01
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| Series: | Neurobiology of Disease |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S096999619890198X |
| _version_ | 1818734837114077184 |
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| author | Kieran R. Williams Ann M. Saunders Allen D. Roses Patricia J. Armati |
| author_facet | Kieran R. Williams Ann M. Saunders Allen D. Roses Patricia J. Armati |
| author_sort | Kieran R. Williams |
| collection | DOAJ |
| description | Apolipoprotein E (apoE) has been confirmed as a risk factor for late-onset Alzheimer's disease (AD) and is associated with neurofibrillary tangles and senile plaques, the microscopic pathological characteristics of AD. There has been no direct evidence that human central nervous system neurons can take up and internalize exogenous apoE, which may be important in order for apoE to be involved in the development of the disease. This paper demonstrates by immunohistochemistry and confocal microscopy that cultured human brain neurons can take up and internalize exogenous recombinant human apoE3. We confirm that neurons express the low-density lipoprotein receptor-related protein (LRP) but do not express the low-density lipoprotein receptor. We also demonstrate that the LRP mediates the neuronal uptake of apoE. |
| first_indexed | 2024-12-18T00:11:42Z |
| format | Article |
| id | doaj.art-1f7e09a23ea44a2da71a8936ba4be5c1 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-18T00:11:42Z |
| publishDate | 1998-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-1f7e09a23ea44a2da71a8936ba4be5c12022-12-21T21:27:40ZengElsevierNeurobiology of Disease1095-953X1998-10-0154271279Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System NeuronsKieran R. Williams0Ann M. Saunders1Allen D. Roses2Patricia J. Armati3Neuroscience Unit, School of Biological Sciences, Building AO8, The University of Sydney, Sydney, New South Wales, 2006, Australia; Department of Medicine (Neurology), Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, North Carolina, 27710Neuroscience Unit, School of Biological Sciences, Building AO8, The University of Sydney, Sydney, New South Wales, 2006, Australia; Department of Medicine (Neurology), Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, North Carolina, 27710Neuroscience Unit, School of Biological Sciences, Building AO8, The University of Sydney, Sydney, New South Wales, 2006, Australia; Department of Medicine (Neurology), Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, North Carolina, 27710Neuroscience Unit, School of Biological Sciences, Building AO8, The University of Sydney, Sydney, New South Wales, 2006, Australia; Department of Medicine (Neurology), Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, North Carolina, 27710Apolipoprotein E (apoE) has been confirmed as a risk factor for late-onset Alzheimer's disease (AD) and is associated with neurofibrillary tangles and senile plaques, the microscopic pathological characteristics of AD. There has been no direct evidence that human central nervous system neurons can take up and internalize exogenous apoE, which may be important in order for apoE to be involved in the development of the disease. This paper demonstrates by immunohistochemistry and confocal microscopy that cultured human brain neurons can take up and internalize exogenous recombinant human apoE3. We confirm that neurons express the low-density lipoprotein receptor-related protein (LRP) but do not express the low-density lipoprotein receptor. We also demonstrate that the LRP mediates the neuronal uptake of apoE.http://www.sciencedirect.com/science/article/pii/S096999619890198X |
| spellingShingle | Kieran R. Williams Ann M. Saunders Allen D. Roses Patricia J. Armati Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons Neurobiology of Disease |
| title | Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons |
| title_full | Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons |
| title_fullStr | Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons |
| title_full_unstemmed | Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons |
| title_short | Uptake and Internalization of Exogenous Apolipoprotein E3 by Cultured Human Central Nervous System Neurons |
| title_sort | uptake and internalization of exogenous apolipoprotein e3 by cultured human central nervous system neurons |
| url | http://www.sciencedirect.com/science/article/pii/S096999619890198X |
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