Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress
Melatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related <i>SlCOMT1</i> gene was cloned from tomato (<i>Solanum lycopersicum</i> Mill. cv. <i>Ails...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1514 |
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| author | Dan-Dan Liu Xiao-Shuai Sun Lin Liu Hong-Di Shi Sui-Yun Chen Da-Ke Zhao |
| author_facet | Dan-Dan Liu Xiao-Shuai Sun Lin Liu Hong-Di Shi Sui-Yun Chen Da-Ke Zhao |
| author_sort | Dan-Dan Liu |
| collection | DOAJ |
| description | Melatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related <i>SlCOMT1</i> gene was cloned from tomato (<i>Solanum lycopersicum</i> Mill. cv. <i>Ailsa Craig</i>), which is highly expressed in fruits compared with other organs. The protein was found to locate in the cytoplasm. Melatonin content in <i>SlCOMT1</i> overexpression transgenic tomato plants was significantly higher than that in wild-type plants. Under 800 mM NaCl stress, the transcript level of <i>SlCOMT1</i> in tomato leaf was positively related to the melatonin contents. Furthermore, compared with that in wild-type plants, levels of superoxide and hydrogen peroxide were lower while the content of proline was higher in <i>SlCOMT1</i> transgenic tomatoes. Therefore, <i>SlCOMT1</i> was closely associated with melatonin biosynthesis confers the significant salt tolerance, providing a clue to cope with the growing global problem of salination in agricultural production. |
| first_indexed | 2024-12-11T10:41:01Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T10:41:01Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1f7e50c9fca14dd8961ce1b9994608202022-12-22T01:10:34ZengMDPI AGMolecules1420-30492019-04-01248151410.3390/molecules24081514molecules24081514Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt StressDan-Dan Liu0Xiao-Shuai Sun1Lin Liu2Hong-Di Shi3Sui-Yun Chen4Da-Ke Zhao5School of Agriculture, Yunnan University, Kunming 650091, ChinaSchool of Agriculture, Yunnan University, Kunming 650091, ChinaSchool of Agriculture, Yunnan University, Kunming 650091, ChinaSchool of Agriculture, Yunnan University, Kunming 650091, ChinaBiocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming 650504, ChinaBiocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming 650504, ChinaMelatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related <i>SlCOMT1</i> gene was cloned from tomato (<i>Solanum lycopersicum</i> Mill. cv. <i>Ailsa Craig</i>), which is highly expressed in fruits compared with other organs. The protein was found to locate in the cytoplasm. Melatonin content in <i>SlCOMT1</i> overexpression transgenic tomato plants was significantly higher than that in wild-type plants. Under 800 mM NaCl stress, the transcript level of <i>SlCOMT1</i> in tomato leaf was positively related to the melatonin contents. Furthermore, compared with that in wild-type plants, levels of superoxide and hydrogen peroxide were lower while the content of proline was higher in <i>SlCOMT1</i> transgenic tomatoes. Therefore, <i>SlCOMT1</i> was closely associated with melatonin biosynthesis confers the significant salt tolerance, providing a clue to cope with the growing global problem of salination in agricultural production.https://www.mdpi.com/1420-3049/24/8/1514tomato<i>SlCOMT1</i>melatoningenetical transformationsalt stress |
| spellingShingle | Dan-Dan Liu Xiao-Shuai Sun Lin Liu Hong-Di Shi Sui-Yun Chen Da-Ke Zhao Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress Molecules tomato <i>SlCOMT1</i> melatonin genetical transformation salt stress |
| title | Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress |
| title_full | Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress |
| title_fullStr | Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress |
| title_full_unstemmed | Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress |
| title_short | Overexpression of the Melatonin Synthesis-Related Gene <i>SlCOMT1</i> Improves the Resistance of Tomato to Salt Stress |
| title_sort | overexpression of the melatonin synthesis related gene i slcomt1 i improves the resistance of tomato to salt stress |
| topic | tomato <i>SlCOMT1</i> melatonin genetical transformation salt stress |
| url | https://www.mdpi.com/1420-3049/24/8/1514 |
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