TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO
Tomato is a major crop in the Mediterranean basin, where the cultivation in the open field is often vulnerable to drought. In order to adapt and survive to naturally occurring cycles of drought stress and recovery, plants employ a coordinated array of physiological, biochemical and molecular respons...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-03-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00371/full |
| _version_ | 1818141585839226880 |
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| author | Paolo eIovieno Paola ePunzo Gianpiero eGuida Carmela eMistretta Michael James Van Oosten Roberta eNurcato Hamed eBostan Chiara eColantuono Antonello eCosta Paolo eBagnaresi Maria Luisa eChiusano Rossella eAlbrizio Pasquale eGiorio Giorgia eBatelli Stefania eGrillo |
| author_facet | Paolo eIovieno Paola ePunzo Gianpiero eGuida Carmela eMistretta Michael James Van Oosten Roberta eNurcato Hamed eBostan Chiara eColantuono Antonello eCosta Paolo eBagnaresi Maria Luisa eChiusano Rossella eAlbrizio Pasquale eGiorio Giorgia eBatelli Stefania eGrillo |
| author_sort | Paolo eIovieno |
| collection | DOAJ |
| description | Tomato is a major crop in the Mediterranean basin, where the cultivation in the open field is often vulnerable to drought. In order to adapt and survive to naturally occurring cycles of drought stress and recovery, plants employ a coordinated array of physiological, biochemical and molecular responses. Transcriptomic studies on tomato responses to drought and subsequent recovery are few in number. As the search for novel traits to improve the genetic tolerance to drought increases, a better understanding of these responses is required. To address this need we designed a study in which we induced two cycles of prolonged drought stress and a single recovery by rewatering in tomato. In order to dissect the complexity of plant responses to drought, we analyzed the physiological responses (stomatal conductance, CO2 assimilation and chlorophyll fluorescence), abscisic acid (ABA) and proline contents. In addition to the physiological and metabolite assays, we generated transcriptomes for multiple points during the stress and recovery cycles. Cluster analysis of differentially expressed genes between the conditions has revealed potential novel components in stress response. The observed reduction in leaf gas exchanges and efficiency of the photosystem PSII was concomitant with a general down-regulation of genes belonging to the photosynthesis, light harvesting and photosystem I and II category induced by drought stress. Gene ontology (GO) categories such as cell proliferation and cell cycle were also significantly enriched in the down-regulated fraction of genes upon drought stress, which may contribute to explain the observed growth reduction. Several histone variants were also repressed during drought stress, indicating that chromatin associated processes are also affected by drought. As expected, ABA accumulated after prolonged water deficit, driving the observed enrichment of stress related GOs in the up-regulated gene fractions, which included transcripts putatively involved in stomatal movements. This transcriptomic study has yielded promising candidate genes that merit further functional studies to confirm their involvement in drought tolerance and recovery. Together, our results contribute to a better understanding of the coordinated responses taking place under drought stress and recovery in adult plants of tomato. |
| first_indexed | 2024-12-11T11:02:13Z |
| format | Article |
| id | doaj.art-1f809abcecf840b6af547a20b16de396 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T11:02:13Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1f809abcecf840b6af547a20b16de3962022-12-22T01:09:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-03-01710.3389/fpls.2016.00371182377TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATOPaolo eIovieno0Paola ePunzo1Gianpiero eGuida2Carmela eMistretta3Michael James Van Oosten4Roberta eNurcato5Hamed eBostan6Chiara eColantuono7Antonello eCosta8Paolo eBagnaresi9Maria Luisa eChiusano10Rossella eAlbrizio11Pasquale eGiorio12Giorgia eBatelli13Stefania eGrillo14National Research Council of ItalyNational Research Council of ItalyNational Research Council of ItalyNational Research Council of ItalyUniversity of Naples “Federico II”National Research Council of ItalyUniversity of Naples “Federico II”University of Naples “Federico II”National Research Council of ItalyConsiglio per la Ricerca e la Sperimentazione in AgricolturaUniversity of Naples “Federico II”National Research Council of ItalyNational Research Council of ItalyNational Research Council of ItalyNational Research Council of ItalyTomato is a major crop in the Mediterranean basin, where the cultivation in the open field is often vulnerable to drought. In order to adapt and survive to naturally occurring cycles of drought stress and recovery, plants employ a coordinated array of physiological, biochemical and molecular responses. Transcriptomic studies on tomato responses to drought and subsequent recovery are few in number. As the search for novel traits to improve the genetic tolerance to drought increases, a better understanding of these responses is required. To address this need we designed a study in which we induced two cycles of prolonged drought stress and a single recovery by rewatering in tomato. In order to dissect the complexity of plant responses to drought, we analyzed the physiological responses (stomatal conductance, CO2 assimilation and chlorophyll fluorescence), abscisic acid (ABA) and proline contents. In addition to the physiological and metabolite assays, we generated transcriptomes for multiple points during the stress and recovery cycles. Cluster analysis of differentially expressed genes between the conditions has revealed potential novel components in stress response. The observed reduction in leaf gas exchanges and efficiency of the photosystem PSII was concomitant with a general down-regulation of genes belonging to the photosynthesis, light harvesting and photosystem I and II category induced by drought stress. Gene ontology (GO) categories such as cell proliferation and cell cycle were also significantly enriched in the down-regulated fraction of genes upon drought stress, which may contribute to explain the observed growth reduction. Several histone variants were also repressed during drought stress, indicating that chromatin associated processes are also affected by drought. As expected, ABA accumulated after prolonged water deficit, driving the observed enrichment of stress related GOs in the up-regulated gene fractions, which included transcripts putatively involved in stomatal movements. This transcriptomic study has yielded promising candidate genes that merit further functional studies to confirm their involvement in drought tolerance and recovery. Together, our results contribute to a better understanding of the coordinated responses taking place under drought stress and recovery in adult plants of tomato.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00371/fullPhotosynthesisProlineABAStomatal conductanceRNA sequencingwater stress. |
| spellingShingle | Paolo eIovieno Paola ePunzo Gianpiero eGuida Carmela eMistretta Michael James Van Oosten Roberta eNurcato Hamed eBostan Chiara eColantuono Antonello eCosta Paolo eBagnaresi Maria Luisa eChiusano Rossella eAlbrizio Pasquale eGiorio Giorgia eBatelli Stefania eGrillo TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO Frontiers in Plant Science Photosynthesis Proline ABA Stomatal conductance RNA sequencing water stress. |
| title | TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO |
| title_full | TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO |
| title_fullStr | TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO |
| title_full_unstemmed | TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO |
| title_short | TRANSCRIPTOMIC CHANGES DRIVE PHYSIOLOGICAL RESPONSES TO PROGRESSIVE DROUGHT STRESS AND REHYDRATION IN TOMATO |
| title_sort | transcriptomic changes drive physiological responses to progressive drought stress and rehydration in tomato |
| topic | Photosynthesis Proline ABA Stomatal conductance RNA sequencing water stress. |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00371/full |
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