Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation
Lead (Pb) toxicity is a growing serious environmental pollution that threatens human health and crop productivity. Poplar, as an important economic and ecological forest species, has the characteristics of fasting growth and accumulating heavy metals, which is a powerful model plant for phytoremedia...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321005224 |
| _version_ | 1818572915789004800 |
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| author | Cong-Cong Shen Mo-Xian Chen Tian Xiao Cheng Zhang Jun Shang Kai-Lu Zhang Fu-Yuan Zhu |
| author_facet | Cong-Cong Shen Mo-Xian Chen Tian Xiao Cheng Zhang Jun Shang Kai-Lu Zhang Fu-Yuan Zhu |
| author_sort | Cong-Cong Shen |
| collection | DOAJ |
| description | Lead (Pb) toxicity is a growing serious environmental pollution that threatens human health and crop productivity. Poplar, as an important economic and ecological forest species, has the characteristics of fasting growth and accumulating heavy metals, which is a powerful model plant for phytoremediation. Here, a novel label-free quantitative proteomic platform of SWATH-MS was applied to detect proteome changes in poplar seedling roots following Pb treatment. In total 4388 unique proteins were identified and quantified, among which 542 proteins showed significant abundance changes upon Pb(II) exposure. Functional categorizations revealed that differentially expressed proteins (DEPs) primarily distributed in specialized biological processes. Particularly, lignin and flavonoid biosynthesis pathway were strongly activated upon Pb exposure, implicating their potential roles for Pb detoxification in poplar. Furthermore, hemicellulose and pectin related cell wall proteins exhibited increased abundances, where may function as a sequestration reservoir to reduce Pb toxicity in cytoplasm. Simultaneously, up-regulation of glutathione metabolism may serve as a protective role for Pb-induced oxidative damages in poplar. Further correlation investigation revealed an extra layer of post-transcriptional regulation during Pb response in poplar. Overall, our work represents multiply potential regulators in mediating Pb tolerance in poplar, providing molecular targets and strategies for phytoremediation. |
| first_indexed | 2024-12-15T00:04:02Z |
| format | Article |
| id | doaj.art-1f82aa11f0854f22a303a5ce6234709e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-15T00:04:02Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-1f82aa11f0854f22a303a5ce6234709e2022-12-21T22:42:48ZengElsevierEcotoxicology and Environmental Safety0147-65132021-09-01220112410Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigationCong-Cong Shen0Mo-Xian Chen1Tian Xiao2Cheng Zhang3Jun Shang4Kai-Lu Zhang5Fu-Yuan Zhu6Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaDepartment of Cell Biology and Genetics, School of Medicine, Shenzhen University, Shenzhen, Guangdong, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, China; International Cultivar Registration Center for Osmanthus, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, ChinaSpecAlly Life Technology Co., Ltd and Wuhan Institute of Biotechnology, Wuhan, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, China; International Cultivar Registration Center for Osmanthus, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China; Correspondence to: College of Biology and the Environment, Nanjing Forestry University, Nanjing, Jiangsu Province, 210037, China.Lead (Pb) toxicity is a growing serious environmental pollution that threatens human health and crop productivity. Poplar, as an important economic and ecological forest species, has the characteristics of fasting growth and accumulating heavy metals, which is a powerful model plant for phytoremediation. Here, a novel label-free quantitative proteomic platform of SWATH-MS was applied to detect proteome changes in poplar seedling roots following Pb treatment. In total 4388 unique proteins were identified and quantified, among which 542 proteins showed significant abundance changes upon Pb(II) exposure. Functional categorizations revealed that differentially expressed proteins (DEPs) primarily distributed in specialized biological processes. Particularly, lignin and flavonoid biosynthesis pathway were strongly activated upon Pb exposure, implicating their potential roles for Pb detoxification in poplar. Furthermore, hemicellulose and pectin related cell wall proteins exhibited increased abundances, where may function as a sequestration reservoir to reduce Pb toxicity in cytoplasm. Simultaneously, up-regulation of glutathione metabolism may serve as a protective role for Pb-induced oxidative damages in poplar. Further correlation investigation revealed an extra layer of post-transcriptional regulation during Pb response in poplar. Overall, our work represents multiply potential regulators in mediating Pb tolerance in poplar, providing molecular targets and strategies for phytoremediation.http://www.sciencedirect.com/science/article/pii/S0147651321005224SWATH-MSPopulus trichocarpaProteomicsLead responsePhytoremediation |
| spellingShingle | Cong-Cong Shen Mo-Xian Chen Tian Xiao Cheng Zhang Jun Shang Kai-Lu Zhang Fu-Yuan Zhu Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation Ecotoxicology and Environmental Safety SWATH-MS Populus trichocarpa Proteomics Lead response Phytoremediation |
| title | Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation |
| title_full | Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation |
| title_fullStr | Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation |
| title_full_unstemmed | Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation |
| title_short | Global proteome response to Pb(II) toxicity in poplar using SWATH-MS-based quantitative proteomics investigation |
| title_sort | global proteome response to pb ii toxicity in poplar using swath ms based quantitative proteomics investigation |
| topic | SWATH-MS Populus trichocarpa Proteomics Lead response Phytoremediation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321005224 |
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