Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda)
Abstract Herbivorous insects often possess the ability to detoxify chemical defenses from their host plants. The fall armyworm (Spodoptera frugiperda), which feeds principally on maize, detoxifies the maize benzoxazinoid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) by stereoselective re-glu...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14551-w |
| _version_ | 1811334242195472384 |
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| author | Bhawana Israni Katrin Luck Samantha C. W. Römhild Bettina Raguschke Natalie Wielsch Yvonne Hupfer Michael Reichelt Aleš Svatoš Jonathan Gershenzon Daniel Giddings Vassão |
| author_facet | Bhawana Israni Katrin Luck Samantha C. W. Römhild Bettina Raguschke Natalie Wielsch Yvonne Hupfer Michael Reichelt Aleš Svatoš Jonathan Gershenzon Daniel Giddings Vassão |
| author_sort | Bhawana Israni |
| collection | DOAJ |
| description | Abstract Herbivorous insects often possess the ability to detoxify chemical defenses from their host plants. The fall armyworm (Spodoptera frugiperda), which feeds principally on maize, detoxifies the maize benzoxazinoid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) by stereoselective re-glucosylation using a UDP-glucosyltransferase, SfUGT33F28. SfUGT33F28 activity is induced by feeding on a DIMBOA-containing diet, but how this induction is regulated is unknown. In the present work, we describe the alternative splicing of the SfUGT33F28 transcript. Variant transcripts are differentially expressed in response to DIMBOA, and this transcriptional response is mediated by an insect aryl hydrocarbon receptor. These variants have large deletions leading to the production of truncated proteins that have no intrinsic UGT activity with DIMBOA but interact with the full-length enzyme to raise or lower its activity. Therefore, the formation of SfUGT33F28 splice variants induces DIMBOA-conjugating UGT activity when DIMBOA is present in the insect diet and represses activity in the absence of this plant defense compound. |
| first_indexed | 2024-04-13T17:04:53Z |
| format | Article |
| id | doaj.art-1f85b9c5afaf41978ee6f850402ebec1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:04:53Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1f85b9c5afaf41978ee6f850402ebec12022-12-22T02:38:30ZengNature PortfolioScientific Reports2045-23222022-06-0112111710.1038/s41598-022-14551-wAlternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda)Bhawana Israni0Katrin Luck1Samantha C. W. Römhild2Bettina Raguschke3Natalie Wielsch4Yvonne Hupfer5Michael Reichelt6Aleš Svatoš7Jonathan Gershenzon8Daniel Giddings Vassão9Max Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyMax Planck Institute for Chemical EcologyAbstract Herbivorous insects often possess the ability to detoxify chemical defenses from their host plants. The fall armyworm (Spodoptera frugiperda), which feeds principally on maize, detoxifies the maize benzoxazinoid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) by stereoselective re-glucosylation using a UDP-glucosyltransferase, SfUGT33F28. SfUGT33F28 activity is induced by feeding on a DIMBOA-containing diet, but how this induction is regulated is unknown. In the present work, we describe the alternative splicing of the SfUGT33F28 transcript. Variant transcripts are differentially expressed in response to DIMBOA, and this transcriptional response is mediated by an insect aryl hydrocarbon receptor. These variants have large deletions leading to the production of truncated proteins that have no intrinsic UGT activity with DIMBOA but interact with the full-length enzyme to raise or lower its activity. Therefore, the formation of SfUGT33F28 splice variants induces DIMBOA-conjugating UGT activity when DIMBOA is present in the insect diet and represses activity in the absence of this plant defense compound.https://doi.org/10.1038/s41598-022-14551-w |
| spellingShingle | Bhawana Israni Katrin Luck Samantha C. W. Römhild Bettina Raguschke Natalie Wielsch Yvonne Hupfer Michael Reichelt Aleš Svatoš Jonathan Gershenzon Daniel Giddings Vassão Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) Scientific Reports |
| title | Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) |
| title_full | Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) |
| title_fullStr | Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) |
| title_full_unstemmed | Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) |
| title_short | Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda) |
| title_sort | alternative transcript splicing regulates udp glucosyltransferase catalyzed detoxification of dimboa in the fall armyworm spodoptera frugiperda |
| url | https://doi.org/10.1038/s41598-022-14551-w |
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