Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs
Porcine respiratory disease is a multifactorial disease that can be influenced by a number of different microorganisms, as well as by non-infectious factors such as the management and environment of the animals. It is generally believed that the interaction between different infectious agents plays...
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MDPI AG
2018-07-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/7/382 |
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| author | Anne-Lie Blomström Xingyu Ye Caroline Fossum Per Wallgren Mikael Berg |
| author_facet | Anne-Lie Blomström Xingyu Ye Caroline Fossum Per Wallgren Mikael Berg |
| author_sort | Anne-Lie Blomström |
| collection | DOAJ |
| description | Porcine respiratory disease is a multifactorial disease that can be influenced by a number of different microorganisms, as well as by non-infectious factors such as the management and environment of the animals. It is generally believed that the interaction between different infectious agents plays an important role in regard to respiratory diseases. Therefore, we used high-throughput sequencing combined with viral metagenomics to characterise the viral community of tonsil samples from pigs coming from a conventional herd with lesions in the respiratory tract at slaughter. In parallel, samples from specific pathogen-free pigs were also analysed. This study showed a variable co-infection rate in the different pigs. The differences were not seen at the group level but in individual pigs. Some viruses such as adenoviruses and certain picornaviruses could be found in most pigs, while others such as different parvoviruses and anelloviruses were only identified in a few pigs. In addition, the complete coding region of porcine parvovirus 7 was obtained, as were the complete genomes of two teschoviruses. The results from this study will aid in elucidating which viruses are circulating in both healthy pigs and in pigs associated with respiratory illness. This knowledge is needed for future investigations into the role of viral-viral interactions in relation to disease development. |
| first_indexed | 2024-12-10T15:27:34Z |
| format | Article |
| id | doaj.art-1f87b5374a584cd28c5a632a0a349d34 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T15:27:34Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1f87b5374a584cd28c5a632a0a349d342022-12-22T01:43:29ZengMDPI AGViruses1999-49152018-07-0110738210.3390/v10070382v10070382Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free PigsAnne-Lie Blomström0Xingyu Ye1Caroline Fossum2Per Wallgren3Mikael Berg4Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07 Uppsala, SwedenNational veterinary institute (SVA), 751 89 Uppsala SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07 Uppsala, SwedenPorcine respiratory disease is a multifactorial disease that can be influenced by a number of different microorganisms, as well as by non-infectious factors such as the management and environment of the animals. It is generally believed that the interaction between different infectious agents plays an important role in regard to respiratory diseases. Therefore, we used high-throughput sequencing combined with viral metagenomics to characterise the viral community of tonsil samples from pigs coming from a conventional herd with lesions in the respiratory tract at slaughter. In parallel, samples from specific pathogen-free pigs were also analysed. This study showed a variable co-infection rate in the different pigs. The differences were not seen at the group level but in individual pigs. Some viruses such as adenoviruses and certain picornaviruses could be found in most pigs, while others such as different parvoviruses and anelloviruses were only identified in a few pigs. In addition, the complete coding region of porcine parvovirus 7 was obtained, as were the complete genomes of two teschoviruses. The results from this study will aid in elucidating which viruses are circulating in both healthy pigs and in pigs associated with respiratory illness. This knowledge is needed for future investigations into the role of viral-viral interactions in relation to disease development.http://www.mdpi.com/1999-4915/10/7/382viral metagenomicspigsrespiratory diseasespecific pathogen-free pigs |
| spellingShingle | Anne-Lie Blomström Xingyu Ye Caroline Fossum Per Wallgren Mikael Berg Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs Viruses viral metagenomics pigs respiratory disease specific pathogen-free pigs |
| title | Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs |
| title_full | Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs |
| title_fullStr | Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs |
| title_full_unstemmed | Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs |
| title_short | Characterisation of the Virome of Tonsils from Conventional Pigs and from Specific Pathogen-Free Pigs |
| title_sort | characterisation of the virome of tonsils from conventional pigs and from specific pathogen free pigs |
| topic | viral metagenomics pigs respiratory disease specific pathogen-free pigs |
| url | http://www.mdpi.com/1999-4915/10/7/382 |
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