Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis
Geo-authentic herbs refer to medicinal materials produced in a specific region with superior quality. <i>Stephania tetrandra</i> S. Moore (<i>S. tetrandra</i>) is cultivated in many provinces of China, including Anhui, Zhejiang, Fujian, Jiangxi, Hunan, Guangxi, Guangdong, Hai...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/21/7224 |
| _version_ | 1797467087250128896 |
|---|---|
| author | Xiunan Cao Xinxin Miao Minglei Ge Mengmeng Zhang Zhenguo Lv Wei Wang Yanxu Chang Huizi Ouyang Jun He |
| author_facet | Xiunan Cao Xinxin Miao Minglei Ge Mengmeng Zhang Zhenguo Lv Wei Wang Yanxu Chang Huizi Ouyang Jun He |
| author_sort | Xiunan Cao |
| collection | DOAJ |
| description | Geo-authentic herbs refer to medicinal materials produced in a specific region with superior quality. <i>Stephania tetrandra</i> S. Moore (<i>S. tetrandra</i>) is cultivated in many provinces of China, including Anhui, Zhejiang, Fujian, Jiangxi, Hunan, Guangxi, Guangdong, Hainan, and Taiwan, among which Jiangxi is the geo-authentic origin. To explore habitat-related chemical markers of herbal medicine, an integrated chromatographic technique including gas chromatography-mass spectrometry (GC-MS), ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) combined with chemometric analysis was established. The established methods manifested that they were clearly divided into two groups according to non-authentic origins and geo-authentic origins, suggesting that the metabolites were closely related to their producing areas. A total of 70 volatile compounds and 50 non-volatile compounds were identified in <i>S. tetrandra</i>. Meanwhile, tetrandrine, fangchinoline, isocorydine, magnocurarine, magnoflorine, boldine, and higenamine as chemical markers were accurately quantified and suggested importance in grouping non-authentic origins and geo-authentic origins samples. The discriminatory analysis also indicated well prediction performance with an accuracy of 80%. The results showed that the multiple chromatographic and chemometric analysis technique could be used as an effective approach for discovering the chemical markers of herbal medicine to fulfill the evaluation of overall chemical consistency among samples from different producing areas. |
| first_indexed | 2024-03-09T18:48:39Z |
| format | Article |
| id | doaj.art-1f87e8928f0e459a8fd859e59c3e07fa |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:48:39Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1f87e8928f0e459a8fd859e59c3e07fa2023-11-24T06:00:39ZengMDPI AGMolecules1420-30492022-10-012721722410.3390/molecules27217224Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric AnalysisXiunan Cao0Xinxin Miao1Minglei Ge2Mengmeng Zhang3Zhenguo Lv4Wei Wang5Yanxu Chang6Huizi Ouyang7Jun He8State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaState Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, ChinaGeo-authentic herbs refer to medicinal materials produced in a specific region with superior quality. <i>Stephania tetrandra</i> S. Moore (<i>S. tetrandra</i>) is cultivated in many provinces of China, including Anhui, Zhejiang, Fujian, Jiangxi, Hunan, Guangxi, Guangdong, Hainan, and Taiwan, among which Jiangxi is the geo-authentic origin. To explore habitat-related chemical markers of herbal medicine, an integrated chromatographic technique including gas chromatography-mass spectrometry (GC-MS), ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) combined with chemometric analysis was established. The established methods manifested that they were clearly divided into two groups according to non-authentic origins and geo-authentic origins, suggesting that the metabolites were closely related to their producing areas. A total of 70 volatile compounds and 50 non-volatile compounds were identified in <i>S. tetrandra</i>. Meanwhile, tetrandrine, fangchinoline, isocorydine, magnocurarine, magnoflorine, boldine, and higenamine as chemical markers were accurately quantified and suggested importance in grouping non-authentic origins and geo-authentic origins samples. The discriminatory analysis also indicated well prediction performance with an accuracy of 80%. The results showed that the multiple chromatographic and chemometric analysis technique could be used as an effective approach for discovering the chemical markers of herbal medicine to fulfill the evaluation of overall chemical consistency among samples from different producing areas.https://www.mdpi.com/1420-3049/27/21/7224<i>Stephania tetrandra</i>habitat-related chemical markersUHPLC-Q-TOF-MS/MSGC-MSchemometric analysis |
| spellingShingle | Xiunan Cao Xinxin Miao Minglei Ge Mengmeng Zhang Zhenguo Lv Wei Wang Yanxu Chang Huizi Ouyang Jun He Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis Molecules <i>Stephania tetrandra</i> habitat-related chemical markers UHPLC-Q-TOF-MS/MS GC-MS chemometric analysis |
| title | Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis |
| title_full | Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis |
| title_fullStr | Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis |
| title_full_unstemmed | Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis |
| title_short | Exploration of Habitat-Related Chemical Markers for <i>Stephania tetrandra</i> Applying Multiple Chromatographic and Chemometric Analysis |
| title_sort | exploration of habitat related chemical markers for i stephania tetrandra i applying multiple chromatographic and chemometric analysis |
| topic | <i>Stephania tetrandra</i> habitat-related chemical markers UHPLC-Q-TOF-MS/MS GC-MS chemometric analysis |
| url | https://www.mdpi.com/1420-3049/27/21/7224 |
| work_keys_str_mv | AT xiunancao explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT xinxinmiao explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT mingleige explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT mengmengzhang explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT zhenguolv explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT weiwang explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT yanxuchang explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT huiziouyang explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis AT junhe explorationofhabitatrelatedchemicalmarkersforistephaniatetrandraiapplyingmultiplechromatographicandchemometricanalysis |